QTL analysis for Malt Quality in DH Lines of Barley (Steptoe × Morex) grown in Iran

In order to study the quantitative variability of malting quality-related traits and to determine the genomic locations which control these traits, an experiment was conducted using one hundred fifty double haploid (DH) lines of barley and their two parents ‛Steptoe’ & ‛Morex’. Protein content (...

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Main Authors: Mohammad Bahman Sadeghi, Wakil Ahmad Sarhadi, Seyed Ali Peighambari, Mohammad Reza Naghavi, Ahmad Kalbasi Ashtari
Format: Article
Language:English
Published: Hasan Eleroğlu 2013-12-01
Series:Turkish Journal of Agriculture: Food Science and Technology
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Online Access:http://www.agrifoodscience.com/index.php/TURJAF/article/view/36
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author Mohammad Bahman Sadeghi
Wakil Ahmad Sarhadi
Seyed Ali Peighambari
Mohammad Reza Naghavi
Ahmad Kalbasi Ashtari
author_facet Mohammad Bahman Sadeghi
Wakil Ahmad Sarhadi
Seyed Ali Peighambari
Mohammad Reza Naghavi
Ahmad Kalbasi Ashtari
author_sort Mohammad Bahman Sadeghi
collection DOAJ
description In order to study the quantitative variability of malting quality-related traits and to determine the genomic locations which control these traits, an experiment was conducted using one hundred fifty double haploid (DH) lines of barley and their two parents ‛Steptoe’ & ‛Morex’. Protein content (%) was measured using Kjeldahl method, diastatic power calculated with Lintner formula, Malt extract was measured for each by special weight achieved and based on Malt Berix Charts. Transgressive segregation in both directions was observed for all traits. Genetic map is fairly saturated and comprising 327 RFLP markers with a total length of 1226.3cM with an average marker spacing of 3.75cM. Seventeen QTLs by LOD≤2(LRS≤9.21) controlling different studied traits were identified for all studied traits. Total phenotypic variance explained by these QTLs varies from 23.2 to 45.05%. Highest LOD scores were obtained for QTL’s controlling diastatic power (Qdip3Ha) on chromosome 3H, and lowest LOD scores were obtained for QTL’s controlling seed yield per plant (Qsyp1Hb) on chromosome 1H. Therefore gain through marker-assisted selection (MAS) in this population would be limited and some of the “Steptoe× Morex” population was developed with the intention of isolating and advancing barley lines for release to the malting quality.
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spelling doaj-art-b89365870cdf4a25b375a9db52ab74312025-08-20T02:19:07ZengHasan EleroğluTurkish Journal of Agriculture: Food Science and Technology2148-127X2013-12-0112566110.24925/turjaf.v1i2.56-61.3621QTL analysis for Malt Quality in DH Lines of Barley (Steptoe × Morex) grown in IranMohammad Bahman SadeghiWakil Ahmad SarhadiSeyed Ali PeighambariMohammad Reza NaghaviAhmad Kalbasi AshtariIn order to study the quantitative variability of malting quality-related traits and to determine the genomic locations which control these traits, an experiment was conducted using one hundred fifty double haploid (DH) lines of barley and their two parents ‛Steptoe’ & ‛Morex’. Protein content (%) was measured using Kjeldahl method, diastatic power calculated with Lintner formula, Malt extract was measured for each by special weight achieved and based on Malt Berix Charts. Transgressive segregation in both directions was observed for all traits. Genetic map is fairly saturated and comprising 327 RFLP markers with a total length of 1226.3cM with an average marker spacing of 3.75cM. Seventeen QTLs by LOD≤2(LRS≤9.21) controlling different studied traits were identified for all studied traits. Total phenotypic variance explained by these QTLs varies from 23.2 to 45.05%. Highest LOD scores were obtained for QTL’s controlling diastatic power (Qdip3Ha) on chromosome 3H, and lowest LOD scores were obtained for QTL’s controlling seed yield per plant (Qsyp1Hb) on chromosome 1H. Therefore gain through marker-assisted selection (MAS) in this population would be limited and some of the “Steptoe× Morex” population was developed with the intention of isolating and advancing barley lines for release to the malting quality.http://www.agrifoodscience.com/index.php/TURJAF/article/view/36Malting quality, QTL, doubled haploid, linkage map, Protein content.
spellingShingle Mohammad Bahman Sadeghi
Wakil Ahmad Sarhadi
Seyed Ali Peighambari
Mohammad Reza Naghavi
Ahmad Kalbasi Ashtari
QTL analysis for Malt Quality in DH Lines of Barley (Steptoe × Morex) grown in Iran
Turkish Journal of Agriculture: Food Science and Technology
Malting quality, QTL, doubled haploid, linkage map, Protein content.
title QTL analysis for Malt Quality in DH Lines of Barley (Steptoe × Morex) grown in Iran
title_full QTL analysis for Malt Quality in DH Lines of Barley (Steptoe × Morex) grown in Iran
title_fullStr QTL analysis for Malt Quality in DH Lines of Barley (Steptoe × Morex) grown in Iran
title_full_unstemmed QTL analysis for Malt Quality in DH Lines of Barley (Steptoe × Morex) grown in Iran
title_short QTL analysis for Malt Quality in DH Lines of Barley (Steptoe × Morex) grown in Iran
title_sort qtl analysis for malt quality in dh lines of barley steptoe morex grown in iran
topic Malting quality, QTL, doubled haploid, linkage map, Protein content.
url http://www.agrifoodscience.com/index.php/TURJAF/article/view/36
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AT seyedalipeighambari qtlanalysisformaltqualityindhlinesofbarleysteptoemorexgrowniniran
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