Three-dimensional quantification of cellular traction forces and mechanosensing of thin substrata by fourier traction force microscopy.
We introduce a novel three-dimensional (3D) traction force microscopy (TFM) method motivated by the recent discovery that cells adhering on plane surfaces exert both in-plane and out-of-plane traction stresses. We measure the 3D deformation of the substratum on a thin layer near its surface, and inp...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0069850 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850128386144337920 |
|---|---|
| author | Juan C del Álamo Ruedi Meili Begoña Álvarez-González Baldomero Alonso-Latorre Effie Bastounis Richard Firtel Juan C Lasheras |
| author_facet | Juan C del Álamo Ruedi Meili Begoña Álvarez-González Baldomero Alonso-Latorre Effie Bastounis Richard Firtel Juan C Lasheras |
| author_sort | Juan C del Álamo |
| collection | DOAJ |
| description | We introduce a novel three-dimensional (3D) traction force microscopy (TFM) method motivated by the recent discovery that cells adhering on plane surfaces exert both in-plane and out-of-plane traction stresses. We measure the 3D deformation of the substratum on a thin layer near its surface, and input this information into an exact analytical solution of the elastic equilibrium equation. These operations are performed in the Fourier domain with high computational efficiency, allowing to obtain the 3D traction stresses from raw microscopy images virtually in real time. We also characterize the error of previous two-dimensional (2D) TFM methods that neglect the out-of-plane component of the traction stresses. This analysis reveals that, under certain combinations of experimental parameters (cell size, substratums' thickness and Poisson's ratio), the accuracy of 2D TFM methods is minimally affected by neglecting the out-of-plane component of the traction stresses. Finally, we consider the cell's mechanosensing of substratum thickness by 3D traction stresses, finding that, when cells adhere on thin substrata, their out-of-plane traction stresses can reach four times deeper into the substratum than their in-plane traction stresses. It is also found that the substratum stiffness sensed by applying out-of-plane traction stresses may be up to 10 times larger than the stiffness sensed by applying in-plane traction stresses. |
| format | Article |
| id | doaj-art-b88e9891dcea49e397f665003b71c15b |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-b88e9891dcea49e397f665003b71c15b2025-08-20T02:33:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0189e6985010.1371/journal.pone.0069850Three-dimensional quantification of cellular traction forces and mechanosensing of thin substrata by fourier traction force microscopy.Juan C del ÁlamoRuedi MeiliBegoña Álvarez-GonzálezBaldomero Alonso-LatorreEffie BastounisRichard FirtelJuan C LasherasWe introduce a novel three-dimensional (3D) traction force microscopy (TFM) method motivated by the recent discovery that cells adhering on plane surfaces exert both in-plane and out-of-plane traction stresses. We measure the 3D deformation of the substratum on a thin layer near its surface, and input this information into an exact analytical solution of the elastic equilibrium equation. These operations are performed in the Fourier domain with high computational efficiency, allowing to obtain the 3D traction stresses from raw microscopy images virtually in real time. We also characterize the error of previous two-dimensional (2D) TFM methods that neglect the out-of-plane component of the traction stresses. This analysis reveals that, under certain combinations of experimental parameters (cell size, substratums' thickness and Poisson's ratio), the accuracy of 2D TFM methods is minimally affected by neglecting the out-of-plane component of the traction stresses. Finally, we consider the cell's mechanosensing of substratum thickness by 3D traction stresses, finding that, when cells adhere on thin substrata, their out-of-plane traction stresses can reach four times deeper into the substratum than their in-plane traction stresses. It is also found that the substratum stiffness sensed by applying out-of-plane traction stresses may be up to 10 times larger than the stiffness sensed by applying in-plane traction stresses.https://doi.org/10.1371/journal.pone.0069850 |
| spellingShingle | Juan C del Álamo Ruedi Meili Begoña Álvarez-González Baldomero Alonso-Latorre Effie Bastounis Richard Firtel Juan C Lasheras Three-dimensional quantification of cellular traction forces and mechanosensing of thin substrata by fourier traction force microscopy. PLoS ONE |
| title | Three-dimensional quantification of cellular traction forces and mechanosensing of thin substrata by fourier traction force microscopy. |
| title_full | Three-dimensional quantification of cellular traction forces and mechanosensing of thin substrata by fourier traction force microscopy. |
| title_fullStr | Three-dimensional quantification of cellular traction forces and mechanosensing of thin substrata by fourier traction force microscopy. |
| title_full_unstemmed | Three-dimensional quantification of cellular traction forces and mechanosensing of thin substrata by fourier traction force microscopy. |
| title_short | Three-dimensional quantification of cellular traction forces and mechanosensing of thin substrata by fourier traction force microscopy. |
| title_sort | three dimensional quantification of cellular traction forces and mechanosensing of thin substrata by fourier traction force microscopy |
| url | https://doi.org/10.1371/journal.pone.0069850 |
| work_keys_str_mv | AT juancdelalamo threedimensionalquantificationofcellulartractionforcesandmechanosensingofthinsubstratabyfouriertractionforcemicroscopy AT ruedimeili threedimensionalquantificationofcellulartractionforcesandmechanosensingofthinsubstratabyfouriertractionforcemicroscopy AT begonaalvarezgonzalez threedimensionalquantificationofcellulartractionforcesandmechanosensingofthinsubstratabyfouriertractionforcemicroscopy AT baldomeroalonsolatorre threedimensionalquantificationofcellulartractionforcesandmechanosensingofthinsubstratabyfouriertractionforcemicroscopy AT effiebastounis threedimensionalquantificationofcellulartractionforcesandmechanosensingofthinsubstratabyfouriertractionforcemicroscopy AT richardfirtel threedimensionalquantificationofcellulartractionforcesandmechanosensingofthinsubstratabyfouriertractionforcemicroscopy AT juanclasheras threedimensionalquantificationofcellulartractionforcesandmechanosensingofthinsubstratabyfouriertractionforcemicroscopy |