Immunochromatographic antibody screening for diagnosis of Trypanosoma cruzi in South Texas meso‐mammals
ABSTRACT American trypanosomiasis (Chagas disease) is caused by the flagellated protozoan Trypanosoma cruzi (T. cruzi), a zoonotic parasite found in meso‐mammal species in South Texas, USA. Chagas disease is of growing concern in vertebrate species, and not well‐understood. Protocols for detection o...
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Wiley
2019-12-01
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| Series: | Wildlife Society Bulletin |
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| Online Access: | https://doi.org/10.1002/wsb.1030 |
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| author | Mathew M. Kramm III Andrea E. Montalvo Israel D. Parker Roel R. Lopez Rodeon Gorchakov Melissa S. Nolan |
| author_facet | Mathew M. Kramm III Andrea E. Montalvo Israel D. Parker Roel R. Lopez Rodeon Gorchakov Melissa S. Nolan |
| author_sort | Mathew M. Kramm III |
| collection | DOAJ |
| description | ABSTRACT American trypanosomiasis (Chagas disease) is caused by the flagellated protozoan Trypanosoma cruzi (T. cruzi), a zoonotic parasite found in meso‐mammal species in South Texas, USA. Chagas disease is of growing concern in vertebrate species, and not well‐understood. Protocols for detection of mammalian T. cruzi infection are available, but have poor specificity and sensitivity and require a secondary disease confirmation assay. The utility of combining an antibody‐based and a DNA‐based assay for simultaneous T. cruzi infection detection has been largely underexplored. Our objectives were to evaluate commercially available single‐use Chembio Diagnostics, Inc. DPP® (Dual Path Platform) immunochromatographic rapid detection assay device for the detection of antibodies to T. cruzi in meso‐mammal species as compared with standard polymerase chain reaction (PCR) testing for T. cruzi DNA. In May and June 2016, we tested the device on whole blood serum from 50 meso‐mammals including raccoons (Procyon lotor), Virginia opossums (Didelphis virginiana), fox squirrels (Sciurus niger), rock squirrels (Otospermophilus variegatus), a striped skunk (Mephitis mephitis), a nine‐banded armadillo (Dasypus novemcinctus), and a ringtail (Bassariscus astutus) in central Texas. Our results showed the Chembio Diagnostics, Inc. DPP® immunochromatographic rapid detect assay system identified infection in 74% of meso‐mammals that tested positive by PCR. This testing device shows promise as an effective first‐line T. cruzi antibody detection device to mass screen for disease infection in meso‐mammals. © 2019 The Wildlife Society. |
| format | Article |
| id | doaj-art-b88c2e809b31438eb6ef699bf5db4135 |
| institution | OA Journals |
| issn | 2328-5540 |
| language | English |
| publishDate | 2019-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Wildlife Society Bulletin |
| spelling | doaj-art-b88c2e809b31438eb6ef699bf5db41352025-08-20T02:36:39ZengWileyWildlife Society Bulletin2328-55402019-12-0143467868210.1002/wsb.1030Immunochromatographic antibody screening for diagnosis of Trypanosoma cruzi in South Texas meso‐mammalsMathew M. Kramm III0Andrea E. Montalvo1Israel D. Parker2Roel R. Lopez3Rodeon Gorchakov4Melissa S. Nolan5Texas A&M University, Department of Wildlife & Fisheries Sciences 534 John Kimbrough College Station TX 77843 USATexas A&M Natural Resources Institute 1919 Oakwell Farms Parkway San Antonio TX 78218 USATexas A&M Natural Resources Institute 1919 Oakwell Farms Parkway San Antonio TX 78218 USATexas A&M Natural Resources Institute 1919 Oakwell Farms Parkway San Antonio TX 78218 USABaylor College of Medicine and Texas Children's Hospital National School of Tropical Medicine 1102 Bates Houston TX 77030 USABaylor College of Medicine and Texas Children's Hospital National School of Tropical Medicine 1102 Bates Houston TX 77030 USAABSTRACT American trypanosomiasis (Chagas disease) is caused by the flagellated protozoan Trypanosoma cruzi (T. cruzi), a zoonotic parasite found in meso‐mammal species in South Texas, USA. Chagas disease is of growing concern in vertebrate species, and not well‐understood. Protocols for detection of mammalian T. cruzi infection are available, but have poor specificity and sensitivity and require a secondary disease confirmation assay. The utility of combining an antibody‐based and a DNA‐based assay for simultaneous T. cruzi infection detection has been largely underexplored. Our objectives were to evaluate commercially available single‐use Chembio Diagnostics, Inc. DPP® (Dual Path Platform) immunochromatographic rapid detection assay device for the detection of antibodies to T. cruzi in meso‐mammal species as compared with standard polymerase chain reaction (PCR) testing for T. cruzi DNA. In May and June 2016, we tested the device on whole blood serum from 50 meso‐mammals including raccoons (Procyon lotor), Virginia opossums (Didelphis virginiana), fox squirrels (Sciurus niger), rock squirrels (Otospermophilus variegatus), a striped skunk (Mephitis mephitis), a nine‐banded armadillo (Dasypus novemcinctus), and a ringtail (Bassariscus astutus) in central Texas. Our results showed the Chembio Diagnostics, Inc. DPP® immunochromatographic rapid detect assay system identified infection in 74% of meso‐mammals that tested positive by PCR. This testing device shows promise as an effective first‐line T. cruzi antibody detection device to mass screen for disease infection in meso‐mammals. © 2019 The Wildlife Society.https://doi.org/10.1002/wsb.1030Chagas diseasemeso‐mammalsTexastriatomine insectsTrypanosoma cruzi |
| spellingShingle | Mathew M. Kramm III Andrea E. Montalvo Israel D. Parker Roel R. Lopez Rodeon Gorchakov Melissa S. Nolan Immunochromatographic antibody screening for diagnosis of Trypanosoma cruzi in South Texas meso‐mammals Wildlife Society Bulletin Chagas disease meso‐mammals Texas triatomine insects Trypanosoma cruzi |
| title | Immunochromatographic antibody screening for diagnosis of Trypanosoma cruzi in South Texas meso‐mammals |
| title_full | Immunochromatographic antibody screening for diagnosis of Trypanosoma cruzi in South Texas meso‐mammals |
| title_fullStr | Immunochromatographic antibody screening for diagnosis of Trypanosoma cruzi in South Texas meso‐mammals |
| title_full_unstemmed | Immunochromatographic antibody screening for diagnosis of Trypanosoma cruzi in South Texas meso‐mammals |
| title_short | Immunochromatographic antibody screening for diagnosis of Trypanosoma cruzi in South Texas meso‐mammals |
| title_sort | immunochromatographic antibody screening for diagnosis of trypanosoma cruzi in south texas meso mammals |
| topic | Chagas disease meso‐mammals Texas triatomine insects Trypanosoma cruzi |
| url | https://doi.org/10.1002/wsb.1030 |
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