Association of miR-149 gene rs2292832 polymorphism with necrotizing enterocolitis in preterm infants
Background. Necrotizing enterocolitis (NEC) is a prevalent and challenging intestinal disease in premature infants, lacking a specific pathogen consistently associated with its occurrence. Effectively preventing and treating NEC to reduce mortality rates remains a significant contemporary challenge...
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Hacettepe University Institute of Child Health
2025-05-01
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| Series: | The Turkish Journal of Pediatrics |
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| Online Access: | https://turkjpediatr.org/article/view/5653 |
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| author | Hong Qiu Xiaojun Wang Yanhong Li Renping Mao Qin Lv |
| author_facet | Hong Qiu Xiaojun Wang Yanhong Li Renping Mao Qin Lv |
| author_sort | Hong Qiu |
| collection | DOAJ |
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Background. Necrotizing enterocolitis (NEC) is a prevalent and challenging intestinal disease in premature infants, lacking a specific pathogen consistently associated with its occurrence. Effectively preventing and treating NEC to reduce mortality rates remains a significant contemporary challenge. The present study aimed to explore the correlation between microRNA-149 gene polymorphism and NEC in premature infants in a Chinese Han population.
Methods. The expression levels of serum miR-149 were determined using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Polymorphism detection of the miR-149 gene rs2292832 polymorphism was performed by polymerase chain reaction. Multivariate logistic regression analysis was employed to investigate the association between the rs2292832 polymorphism and risk factors for NEC in preterm infants.
Results. General clinical data were compared between 102 preterm infants diagnosed with NEC and 263 preterm infants without NEC. Significant differences were observed in gestational age and birth weight. However, no significant differences were found in antenatal steroid use, sex, or feeding patterns between the two groups. The expression level of serum miR-149 was significantly reduced in premature infants with NEC, and there were differences in the allele frequency of the miR-149 rs2292832 polymorphism between the NEC group and control group. Specifically, the T allele and TT genotype of rs2292832 were associated with an increased susceptibility to NEC. Furthermore, both gestational age and the rs2292832 polymorphism showed a significant association with NEC risk, with the rs2292832 polymorphism of miR-149 being identified as the most prominent risk factor for NEC development in preterm infants.
Conclusions. The rs2292832 gene polymorphism of miR-149 may potentially exert an influence on susceptibility to NEC.
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| format | Article |
| id | doaj-art-b865c8a7c45c4ecc90398a699be8a3b4 |
| institution | OA Journals |
| issn | 0041-4301 2791-6421 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Hacettepe University Institute of Child Health |
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| series | The Turkish Journal of Pediatrics |
| spelling | doaj-art-b865c8a7c45c4ecc90398a699be8a3b42025-08-20T01:59:22ZengHacettepe University Institute of Child HealthThe Turkish Journal of Pediatrics0041-43012791-64212025-05-0167210.24953/turkjpediatr.2025.5653Association of miR-149 gene rs2292832 polymorphism with necrotizing enterocolitis in preterm infantsHong Qiu0https://orcid.org/0009-0004-4415-9926Xiaojun Wang1https://orcid.org/0009-0009-7380-6332Yanhong Li2https://orcid.org/0009-0004-5855-1128Renping Mao3https://orcid.org/0009-0001-8132-4597Qin Lv4https://orcid.org/0009-0008-1146-9396Neonatal Intensive Care Unit, Women and Children’s Hospital of Ningbo University, Ningbo, ChinaNeonatal Intensive Care Unit, Women and Children’s Hospital of Ningbo University, Ningbo, ChinaNeonatal Intensive Care Unit, Women and Children’s Hospital of Ningbo University, Ningbo, ChinaNeonatal Intensive Care Unit, Women and Children’s Hospital of Ningbo University, Ningbo, ChinaNeonatal Intensive Care Unit, Women and Children’s Hospital of Ningbo University, Ningbo, China Background. Necrotizing enterocolitis (NEC) is a prevalent and challenging intestinal disease in premature infants, lacking a specific pathogen consistently associated with its occurrence. Effectively preventing and treating NEC to reduce mortality rates remains a significant contemporary challenge. The present study aimed to explore the correlation between microRNA-149 gene polymorphism and NEC in premature infants in a Chinese Han population. Methods. The expression levels of serum miR-149 were determined using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Polymorphism detection of the miR-149 gene rs2292832 polymorphism was performed by polymerase chain reaction. Multivariate logistic regression analysis was employed to investigate the association between the rs2292832 polymorphism and risk factors for NEC in preterm infants. Results. General clinical data were compared between 102 preterm infants diagnosed with NEC and 263 preterm infants without NEC. Significant differences were observed in gestational age and birth weight. However, no significant differences were found in antenatal steroid use, sex, or feeding patterns between the two groups. The expression level of serum miR-149 was significantly reduced in premature infants with NEC, and there were differences in the allele frequency of the miR-149 rs2292832 polymorphism between the NEC group and control group. Specifically, the T allele and TT genotype of rs2292832 were associated with an increased susceptibility to NEC. Furthermore, both gestational age and the rs2292832 polymorphism showed a significant association with NEC risk, with the rs2292832 polymorphism of miR-149 being identified as the most prominent risk factor for NEC development in preterm infants. Conclusions. The rs2292832 gene polymorphism of miR-149 may potentially exert an influence on susceptibility to NEC. https://turkjpediatr.org/article/view/5653Necrotizing enterocolitis (NEC)Gene polymorphismMiR-149rs2292832 |
| spellingShingle | Hong Qiu Xiaojun Wang Yanhong Li Renping Mao Qin Lv Association of miR-149 gene rs2292832 polymorphism with necrotizing enterocolitis in preterm infants The Turkish Journal of Pediatrics Necrotizing enterocolitis (NEC) Gene polymorphism MiR-149 rs2292832 |
| title | Association of miR-149 gene rs2292832 polymorphism with necrotizing enterocolitis in preterm infants |
| title_full | Association of miR-149 gene rs2292832 polymorphism with necrotizing enterocolitis in preterm infants |
| title_fullStr | Association of miR-149 gene rs2292832 polymorphism with necrotizing enterocolitis in preterm infants |
| title_full_unstemmed | Association of miR-149 gene rs2292832 polymorphism with necrotizing enterocolitis in preterm infants |
| title_short | Association of miR-149 gene rs2292832 polymorphism with necrotizing enterocolitis in preterm infants |
| title_sort | association of mir 149 gene rs2292832 polymorphism with necrotizing enterocolitis in preterm infants |
| topic | Necrotizing enterocolitis (NEC) Gene polymorphism MiR-149 rs2292832 |
| url | https://turkjpediatr.org/article/view/5653 |
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