Continuous Cultivation of <i>Yarrowia lipolytica</i>: Potential, Challenges, and Case Studies
Currently, the yeast <i>Yarrowia lipolytica</i> is regarded as one of the most promising producers of protein, lipids, polyols, organic acids, and other metabolites. The objective of enhancing the efficiency of the target product biosynthesis can be achieved through the improvement of th...
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MDPI AG
2024-12-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/10/12/619 |
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| author | Svetlana V. Kamzolova |
| author_facet | Svetlana V. Kamzolova |
| author_sort | Svetlana V. Kamzolova |
| collection | DOAJ |
| description | Currently, the yeast <i>Yarrowia lipolytica</i> is regarded as one of the most promising producers of protein, lipids, polyols, organic acids, and other metabolites. The objective of enhancing the efficiency of the target product biosynthesis can be achieved through the improvement of the strains-producers and the optimization of the cultivation conditions. The present review assesses the potential of continuous cultivation methods (chemostat, turbidostat, pH-auxostat, changestats, etc.) in order to gain insight into the impact of strains and cultivation conditions on the productivity of the developed bioprocesses. The utilization of continuous cultivation methods enables the implementation of processes under controlled and reproducible conditions, thus stabilizing the parameters of the cultivation and the physiological state of the producer, and obtaining homogeneous samples. The review focuses on nitrogen-limited chemostat cultures, which represent the most commonly employed strategy for investigating the physiological and biochemical characteristics of the yeast <i>Y. lipolytica</i> and for developing the processes for the production of lipids, erythritol, citric acid, and recombinant proteins. To date, such an analysis of the literature has not been conducted in the context of the yeast <i>Y. lipolytica</i>. |
| format | Article |
| id | doaj-art-b861c8ffcedb41a29dbf8da7430d7062 |
| institution | DOAJ |
| issn | 2311-5637 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj-art-b861c8ffcedb41a29dbf8da7430d70622025-08-20T02:55:32ZengMDPI AGFermentation2311-56372024-12-01101261910.3390/fermentation10120619Continuous Cultivation of <i>Yarrowia lipolytica</i>: Potential, Challenges, and Case StudiesSvetlana V. Kamzolova0G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Center for Biological Research of the Russian Academy of Sciences, Prospect Nauki 5, Pushchino 142290, RussiaCurrently, the yeast <i>Yarrowia lipolytica</i> is regarded as one of the most promising producers of protein, lipids, polyols, organic acids, and other metabolites. The objective of enhancing the efficiency of the target product biosynthesis can be achieved through the improvement of the strains-producers and the optimization of the cultivation conditions. The present review assesses the potential of continuous cultivation methods (chemostat, turbidostat, pH-auxostat, changestats, etc.) in order to gain insight into the impact of strains and cultivation conditions on the productivity of the developed bioprocesses. The utilization of continuous cultivation methods enables the implementation of processes under controlled and reproducible conditions, thus stabilizing the parameters of the cultivation and the physiological state of the producer, and obtaining homogeneous samples. The review focuses on nitrogen-limited chemostat cultures, which represent the most commonly employed strategy for investigating the physiological and biochemical characteristics of the yeast <i>Y. lipolytica</i> and for developing the processes for the production of lipids, erythritol, citric acid, and recombinant proteins. To date, such an analysis of the literature has not been conducted in the context of the yeast <i>Y. lipolytica</i>.https://www.mdpi.com/2311-5637/10/12/619<i>Yarrowia lipolytica</i> yeastmicrobial synthesiscontinuous cultivationsteady statecitric acidsingle cell oil |
| spellingShingle | Svetlana V. Kamzolova Continuous Cultivation of <i>Yarrowia lipolytica</i>: Potential, Challenges, and Case Studies Fermentation <i>Yarrowia lipolytica</i> yeast microbial synthesis continuous cultivation steady state citric acid single cell oil |
| title | Continuous Cultivation of <i>Yarrowia lipolytica</i>: Potential, Challenges, and Case Studies |
| title_full | Continuous Cultivation of <i>Yarrowia lipolytica</i>: Potential, Challenges, and Case Studies |
| title_fullStr | Continuous Cultivation of <i>Yarrowia lipolytica</i>: Potential, Challenges, and Case Studies |
| title_full_unstemmed | Continuous Cultivation of <i>Yarrowia lipolytica</i>: Potential, Challenges, and Case Studies |
| title_short | Continuous Cultivation of <i>Yarrowia lipolytica</i>: Potential, Challenges, and Case Studies |
| title_sort | continuous cultivation of i yarrowia lipolytica i potential challenges and case studies |
| topic | <i>Yarrowia lipolytica</i> yeast microbial synthesis continuous cultivation steady state citric acid single cell oil |
| url | https://www.mdpi.com/2311-5637/10/12/619 |
| work_keys_str_mv | AT svetlanavkamzolova continuouscultivationofiyarrowialipolyticaipotentialchallengesandcasestudies |