Certain Aspects of Mast Cell Carboxypeptidase A3 Involvement in the Pathogenesis of COVID-19
The objective: to determine the involvement of lung mast cell carboxypeptidase A3 (SPA3) in the pathogenesis of COVID-19. Subjects and Methods. Samples of autopsy specimens from the lungs of patients who died of severe COVID-19 and patients who died of external causes were examined. On day 2 after t...
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| Format: | Article |
| Language: | Russian |
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New Terra Publishing House
2024-03-01
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| Series: | Туберкулез и болезни лёгких |
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| Online Access: | https://www.tibl-journal.com/jour/article/view/1790 |
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| author | A. V. Budnevskiy S. N. Avdeev E. S. Ovsyannikov N. G. Alekseeva V. V. Shishkina I. A. Savushkina I. M. Perveeva V. R. Drobysheva |
| author_facet | A. V. Budnevskiy S. N. Avdeev E. S. Ovsyannikov N. G. Alekseeva V. V. Shishkina I. A. Savushkina I. M. Perveeva V. R. Drobysheva |
| author_sort | A. V. Budnevskiy |
| collection | DOAJ |
| description | The objective: to determine the involvement of lung mast cell carboxypeptidase A3 (SPA3) in the pathogenesis of COVID-19. Subjects and Methods. Samples of autopsy specimens from the lungs of patients who died of severe COVID-19 and patients who died of external causes were examined. On day 2 after the patient's death, the autopsy was performed and autopsy specimens were collected. Histological samples were prepared to analyze the degranulation activity of CPA3-positive mast cells (MCs). Correlations between protease profile and clinical and laboratory parameters were analyzed.Results. In the patients died of COVID-19, the total numbers of CPA3-positive MCs, CPA3-positive MCs with signs of degranulation, and coadjacent CPA3-positive MCs (two CPA3-positive MCs sharing a common cytoplasm) were statistically significantly higher versus the patients from Control Group. Positive correlations were established between the total number of CPA3-positive MCs and CPA3-positive MCs with signs of degranulation with blood hemoglobin level (r = 0.491; p = 0.008; r = 0.521; p = 0.004), between the total number of CPA3-positive MCs degranulating CPA3-positive MCs and adjacent CPA3-positive MCs with total blood protein (r = 0.431, p = 0.020; r = 0.449, p = 0.015; r = 0.456, p = 0.013), and between CPA3-positive MCs with signs of degranulation and total number of CPA3-positive MCs with aPTT level (r = 0.428; p = 0.021; r = 0.374; p = 0.045). Negative correlations were established between the total number of CPA3-positive MCs and the level of INR (r = 0.812; p = 0.050), separately lying CPA3-positive MCs with the number of monocytes (r = 0.384; p = 0.044), jointly adjacent CPA3-positive Mcs with blood eosinophils (r = 0.420, p = 0.023). |
| format | Article |
| id | doaj-art-b83ef860f06f484096af664ff16a9a3e |
| institution | OA Journals |
| issn | 2075-1230 2542-1506 |
| language | Russian |
| publishDate | 2024-03-01 |
| publisher | New Terra Publishing House |
| record_format | Article |
| series | Туберкулез и болезни лёгких |
| spelling | doaj-art-b83ef860f06f484096af664ff16a9a3e2025-08-20T02:16:30ZrusNew Terra Publishing HouseТуберкулез и болезни лёгких2075-12302542-15062024-03-011021263310.58838/2075-1230-2024-102-1-26-331785Certain Aspects of Mast Cell Carboxypeptidase A3 Involvement in the Pathogenesis of COVID-19A. V. Budnevskiy0S. N. Avdeev1E. S. Ovsyannikov2N. G. Alekseeva3V. V. Shishkina4I. A. Savushkina5I. M. Perveeva6V. R. Drobysheva7Voronezh State Medical Academy Named after N.N. Burdenko, Russian Ministry of HealthI.M. Sechenov First Moscow State Medical University (Sechenov University), Russian Ministry of HealthVoronezh State Medical Academy Named after N.N. Burdenko, Russian Ministry of HealthVoronezh State Medical Academy Named after N.N. Burdenko, Russian Ministry of HealthVoronezh State Medical Academy Named after N.N. Burdenko, Russian Ministry of HealthVoronezh State Medical Academy Named after N.N. Burdenko, Russian Ministry of HealthVoronezh Regional Clinical Hospital no.1Voronezh State Medical Academy Named after N.N. Burdenko, Russian Ministry of HealthThe objective: to determine the involvement of lung mast cell carboxypeptidase A3 (SPA3) in the pathogenesis of COVID-19. Subjects and Methods. Samples of autopsy specimens from the lungs of patients who died of severe COVID-19 and patients who died of external causes were examined. On day 2 after the patient's death, the autopsy was performed and autopsy specimens were collected. Histological samples were prepared to analyze the degranulation activity of CPA3-positive mast cells (MCs). Correlations between protease profile and clinical and laboratory parameters were analyzed.Results. In the patients died of COVID-19, the total numbers of CPA3-positive MCs, CPA3-positive MCs with signs of degranulation, and coadjacent CPA3-positive MCs (two CPA3-positive MCs sharing a common cytoplasm) were statistically significantly higher versus the patients from Control Group. Positive correlations were established between the total number of CPA3-positive MCs and CPA3-positive MCs with signs of degranulation with blood hemoglobin level (r = 0.491; p = 0.008; r = 0.521; p = 0.004), between the total number of CPA3-positive MCs degranulating CPA3-positive MCs and adjacent CPA3-positive MCs with total blood protein (r = 0.431, p = 0.020; r = 0.449, p = 0.015; r = 0.456, p = 0.013), and between CPA3-positive MCs with signs of degranulation and total number of CPA3-positive MCs with aPTT level (r = 0.428; p = 0.021; r = 0.374; p = 0.045). Negative correlations were established between the total number of CPA3-positive MCs and the level of INR (r = 0.812; p = 0.050), separately lying CPA3-positive MCs with the number of monocytes (r = 0.384; p = 0.044), jointly adjacent CPA3-positive Mcs with blood eosinophils (r = 0.420, p = 0.023).https://www.tibl-journal.com/jour/article/view/1790mast cellscovid-19lung damagecarboxypeptidase a3 |
| spellingShingle | A. V. Budnevskiy S. N. Avdeev E. S. Ovsyannikov N. G. Alekseeva V. V. Shishkina I. A. Savushkina I. M. Perveeva V. R. Drobysheva Certain Aspects of Mast Cell Carboxypeptidase A3 Involvement in the Pathogenesis of COVID-19 Туберкулез и болезни лёгких mast cells covid-19 lung damage carboxypeptidase a3 |
| title | Certain Aspects of Mast Cell Carboxypeptidase A3 Involvement in the Pathogenesis of COVID-19 |
| title_full | Certain Aspects of Mast Cell Carboxypeptidase A3 Involvement in the Pathogenesis of COVID-19 |
| title_fullStr | Certain Aspects of Mast Cell Carboxypeptidase A3 Involvement in the Pathogenesis of COVID-19 |
| title_full_unstemmed | Certain Aspects of Mast Cell Carboxypeptidase A3 Involvement in the Pathogenesis of COVID-19 |
| title_short | Certain Aspects of Mast Cell Carboxypeptidase A3 Involvement in the Pathogenesis of COVID-19 |
| title_sort | certain aspects of mast cell carboxypeptidase a3 involvement in the pathogenesis of covid 19 |
| topic | mast cells covid-19 lung damage carboxypeptidase a3 |
| url | https://www.tibl-journal.com/jour/article/view/1790 |
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