Efficacy of Combined Use of PELNAC and Platelet-rich Plasma in Promoting Wound Healing
Background:. Artificial dermis is useful for treating full-thickness skin defects but requires a long time to form dermis-like granulation tissue. This study investigated whether platelet-rich plasma (PRP) combined with PELNAC, a collagen-based artificial dermis, promotes wound healing and examined...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer
2025-08-01
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| Series: | Plastic and Reconstructive Surgery, Global Open |
| Online Access: | http://journals.lww.com/prsgo/fulltext/10.1097/GOX.0000000000007062 |
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| author | Hiroshi Fujimaki, MD, PhD Hajime Matsumine, MD, PhD Yuki Hasegawa, MD, PhD Yosuke Niimi, MD, PhD Hiroyuki Sakurai, MD, PhD |
| author_facet | Hiroshi Fujimaki, MD, PhD Hajime Matsumine, MD, PhD Yuki Hasegawa, MD, PhD Yosuke Niimi, MD, PhD Hiroyuki Sakurai, MD, PhD |
| author_sort | Hiroshi Fujimaki, MD, PhD |
| collection | DOAJ |
| description | Background:. Artificial dermis is useful for treating full-thickness skin defects but requires a long time to form dermis-like granulation tissue. This study investigated whether platelet-rich plasma (PRP) combined with PELNAC, a collagen-based artificial dermis, promotes wound healing and examined the underlying mechanism.
Methods:. Two 1-cm² full-thickness skin defects were created in 8-week-old male nude rats (n = 8). One defect received saline-impregnated PELNAC (control group); the other received PRP-impregnated PELNAC (combination group). Cytokine levels in PRP were measured. Wound area measurements and histological and immunohistochemical evaluations (transforming growth factor-β1 [TGF-β1], vascular endothelial growth factor [VEGF], α-smooth muscle actin, and von Willebrand factor) were performed on day 14.
Results:. Compared with the control group, the combination group showed a significantly reduced wound area (32.4 ± 14.89 versus 68.3 ± 14.9 mm², P < 0.001) and increased dermis-like granulation tissue thickness (947 ± 219 versus 448 ± 165 μm, P < 0.001). Expression of TGF-β1 (8.9% ± 1.7% versus 4.2% ± 1.2%, P < 0.0001) and VEGF (4.4% ± 1.2% versus 1.9% ± 0.5%, P < 0.0001) was significantly higher in the combination group, whereas α-smooth muscle actin–positive cells and von Willebrand factor–positive blood vessels showed no significant differences.
Conclusions:. The combined use of PELNAC and PRP accelerated dermis-like granulation tissue formation through increased expression of VEGF and transforming growth factor-β1, significantly improving wound healing. This combination therapy could be easily applied in clinical practice and may contribute to shortening healing times for deep dermal defects. |
| format | Article |
| id | doaj-art-b83b03a8a8f140d2af43068ab3d462b9 |
| institution | Kabale University |
| issn | 2169-7574 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Wolters Kluwer |
| record_format | Article |
| series | Plastic and Reconstructive Surgery, Global Open |
| spelling | doaj-art-b83b03a8a8f140d2af43068ab3d462b92025-08-26T03:24:29ZengWolters KluwerPlastic and Reconstructive Surgery, Global Open2169-75742025-08-01138e706210.1097/GOX.0000000000007062202508000-00036Efficacy of Combined Use of PELNAC and Platelet-rich Plasma in Promoting Wound HealingHiroshi Fujimaki, MD, PhD0Hajime Matsumine, MD, PhD1Yuki Hasegawa, MD, PhD2Yosuke Niimi, MD, PhD3Hiroyuki Sakurai, MD, PhD4From the * Department of Plastic and Reconstructive Surgery, Tokyo Women’s Medical University, Tokyo, JapanFrom the * Department of Plastic and Reconstructive Surgery, Tokyo Women’s Medical University, Tokyo, JapanFrom the * Department of Plastic and Reconstructive Surgery, Tokyo Women’s Medical University, Tokyo, JapanFrom the * Department of Plastic and Reconstructive Surgery, Tokyo Women’s Medical University, Tokyo, JapanFrom the * Department of Plastic and Reconstructive Surgery, Tokyo Women’s Medical University, Tokyo, JapanBackground:. Artificial dermis is useful for treating full-thickness skin defects but requires a long time to form dermis-like granulation tissue. This study investigated whether platelet-rich plasma (PRP) combined with PELNAC, a collagen-based artificial dermis, promotes wound healing and examined the underlying mechanism. Methods:. Two 1-cm² full-thickness skin defects were created in 8-week-old male nude rats (n = 8). One defect received saline-impregnated PELNAC (control group); the other received PRP-impregnated PELNAC (combination group). Cytokine levels in PRP were measured. Wound area measurements and histological and immunohistochemical evaluations (transforming growth factor-β1 [TGF-β1], vascular endothelial growth factor [VEGF], α-smooth muscle actin, and von Willebrand factor) were performed on day 14. Results:. Compared with the control group, the combination group showed a significantly reduced wound area (32.4 ± 14.89 versus 68.3 ± 14.9 mm², P < 0.001) and increased dermis-like granulation tissue thickness (947 ± 219 versus 448 ± 165 μm, P < 0.001). Expression of TGF-β1 (8.9% ± 1.7% versus 4.2% ± 1.2%, P < 0.0001) and VEGF (4.4% ± 1.2% versus 1.9% ± 0.5%, P < 0.0001) was significantly higher in the combination group, whereas α-smooth muscle actin–positive cells and von Willebrand factor–positive blood vessels showed no significant differences. Conclusions:. The combined use of PELNAC and PRP accelerated dermis-like granulation tissue formation through increased expression of VEGF and transforming growth factor-β1, significantly improving wound healing. This combination therapy could be easily applied in clinical practice and may contribute to shortening healing times for deep dermal defects.http://journals.lww.com/prsgo/fulltext/10.1097/GOX.0000000000007062 |
| spellingShingle | Hiroshi Fujimaki, MD, PhD Hajime Matsumine, MD, PhD Yuki Hasegawa, MD, PhD Yosuke Niimi, MD, PhD Hiroyuki Sakurai, MD, PhD Efficacy of Combined Use of PELNAC and Platelet-rich Plasma in Promoting Wound Healing Plastic and Reconstructive Surgery, Global Open |
| title | Efficacy of Combined Use of PELNAC and Platelet-rich Plasma in Promoting Wound Healing |
| title_full | Efficacy of Combined Use of PELNAC and Platelet-rich Plasma in Promoting Wound Healing |
| title_fullStr | Efficacy of Combined Use of PELNAC and Platelet-rich Plasma in Promoting Wound Healing |
| title_full_unstemmed | Efficacy of Combined Use of PELNAC and Platelet-rich Plasma in Promoting Wound Healing |
| title_short | Efficacy of Combined Use of PELNAC and Platelet-rich Plasma in Promoting Wound Healing |
| title_sort | efficacy of combined use of pelnac and platelet rich plasma in promoting wound healing |
| url | http://journals.lww.com/prsgo/fulltext/10.1097/GOX.0000000000007062 |
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