Exploiting of green synthesized silver nanoparticles using Capparis spinosa L. Fruit for spectrophotometric determination of diphenhydramine HCl in pure forms and commercial products

A novel, sensitive, economical, and environmentally friendly method is described for the quantitative determination of diphenhydramine hydrochloride (DPH). The method is based on the preparation of silver nanoparticles (AgNPs) from the extract of Capparis spinosa L. (CPL) fruits to determine DPH as...

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Main Authors: Fadam M. Abdoon, Hasan M. Hasan, Sarhan A. Salman, Suham T. Ameen, Mequanint Birhan
Format: Article
Language:English
Published: Taylor & Francis Group 2023-12-01
Series:Journal of Experimental Nanoscience
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Online Access:https://www.tandfonline.com/doi/10.1080/17458080.2022.2161525
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author Fadam M. Abdoon
Hasan M. Hasan
Sarhan A. Salman
Suham T. Ameen
Mequanint Birhan
author_facet Fadam M. Abdoon
Hasan M. Hasan
Sarhan A. Salman
Suham T. Ameen
Mequanint Birhan
author_sort Fadam M. Abdoon
collection DOAJ
description A novel, sensitive, economical, and environmentally friendly method is described for the quantitative determination of diphenhydramine hydrochloride (DPH). The method is based on the preparation of silver nanoparticles (AgNPs) from the extract of Capparis spinosa L. (CPL) fruits to determine DPH as one of the antihistamine drugs. The colour intensity of the solution increases with increasing DPH concentrations due to the interaction of AgNPs with DPH. FTIR, X-ray, EDX, SEM, and UV-Vis techniques characterised the prepared nanoparticles. The current method presented a linear relationship over the concentration range of 2–45 µg/mL at a wavelength of 415 nm. The detection limit (LOD) and quantitation limit(LOQ) are studied, and the values ​​are 0.36 and 1.19 µg/mL, respectively. The recovery percentage (accuracy function) is more than 99.5%, and the relative standard deviation (RSD%, as a precision function) was 0.73%. The molar absorptivity and Sandel’s sensitivity index are 5.14 × 103 L.mol−1.cm−1 and 0.057 µg.cm−2, respectively. The data were compared to those from other previous approaches using t-test and F-test with a 95% confidence level and found to be 2.107 and 3.18, respectively. According to the ICH guidelines (2005), the proposed method has been validated and provides promising results for determination of DPH in pure form and pharmaceutical preparations.
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spelling doaj-art-b8309a40aaac45f39c20e10d45ce140e2025-08-20T02:49:30ZengTaylor & Francis GroupJournal of Experimental Nanoscience1745-80801745-80992023-12-0118110.1080/17458080.2022.2161525Exploiting of green synthesized silver nanoparticles using Capparis spinosa L. Fruit for spectrophotometric determination of diphenhydramine HCl in pure forms and commercial productsFadam M. Abdoon0Hasan M. Hasan1Sarhan A. Salman2Suham T. Ameen3Mequanint Birhan4Department of Chemistry, College of Science, Tikrit University, Tikrit, IraqCollege of Pharmacy, Tikrit University, Tikrit, IraqDepartment of Chemistry, College of Science, Tikrit University, Tikrit, IraqCollege of Health and Medical Technology, Uruk University, Baghdad, IraqDepartment of Mechanical Engineering, Mizan-Tepi University, Tepi bushira, EthiopiaA novel, sensitive, economical, and environmentally friendly method is described for the quantitative determination of diphenhydramine hydrochloride (DPH). The method is based on the preparation of silver nanoparticles (AgNPs) from the extract of Capparis spinosa L. (CPL) fruits to determine DPH as one of the antihistamine drugs. The colour intensity of the solution increases with increasing DPH concentrations due to the interaction of AgNPs with DPH. FTIR, X-ray, EDX, SEM, and UV-Vis techniques characterised the prepared nanoparticles. The current method presented a linear relationship over the concentration range of 2–45 µg/mL at a wavelength of 415 nm. The detection limit (LOD) and quantitation limit(LOQ) are studied, and the values ​​are 0.36 and 1.19 µg/mL, respectively. The recovery percentage (accuracy function) is more than 99.5%, and the relative standard deviation (RSD%, as a precision function) was 0.73%. The molar absorptivity and Sandel’s sensitivity index are 5.14 × 103 L.mol−1.cm−1 and 0.057 µg.cm−2, respectively. The data were compared to those from other previous approaches using t-test and F-test with a 95% confidence level and found to be 2.107 and 3.18, respectively. According to the ICH guidelines (2005), the proposed method has been validated and provides promising results for determination of DPH in pure form and pharmaceutical preparations.https://www.tandfonline.com/doi/10.1080/17458080.2022.2161525Diphenhydramine HClGreen synthesisedAgNPsCapparis Spinosa L. FruitSpectroscopy
spellingShingle Fadam M. Abdoon
Hasan M. Hasan
Sarhan A. Salman
Suham T. Ameen
Mequanint Birhan
Exploiting of green synthesized silver nanoparticles using Capparis spinosa L. Fruit for spectrophotometric determination of diphenhydramine HCl in pure forms and commercial products
Journal of Experimental Nanoscience
Diphenhydramine HCl
Green synthesised
AgNPs
Capparis Spinosa L. Fruit
Spectroscopy
title Exploiting of green synthesized silver nanoparticles using Capparis spinosa L. Fruit for spectrophotometric determination of diphenhydramine HCl in pure forms and commercial products
title_full Exploiting of green synthesized silver nanoparticles using Capparis spinosa L. Fruit for spectrophotometric determination of diphenhydramine HCl in pure forms and commercial products
title_fullStr Exploiting of green synthesized silver nanoparticles using Capparis spinosa L. Fruit for spectrophotometric determination of diphenhydramine HCl in pure forms and commercial products
title_full_unstemmed Exploiting of green synthesized silver nanoparticles using Capparis spinosa L. Fruit for spectrophotometric determination of diphenhydramine HCl in pure forms and commercial products
title_short Exploiting of green synthesized silver nanoparticles using Capparis spinosa L. Fruit for spectrophotometric determination of diphenhydramine HCl in pure forms and commercial products
title_sort exploiting of green synthesized silver nanoparticles using capparis spinosa l fruit for spectrophotometric determination of diphenhydramine hcl in pure forms and commercial products
topic Diphenhydramine HCl
Green synthesised
AgNPs
Capparis Spinosa L. Fruit
Spectroscopy
url https://www.tandfonline.com/doi/10.1080/17458080.2022.2161525
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