<i>In Vitro</i> Antibacterial Activities and Calf Thymus DNA–Bovine Serum Albumin Interactions of Tridentate NNO Hydrazone Schiff Base–Metal Complexes
Their demonstrable bioactive characteristics, coupled with their wide structural diversity and coordination versatility, render Schiff bases and their coordination complexes biologically active compounds demonstrating outstanding properties. This research describes the synthesis and characterization...
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MDPI AG
2025-06-01
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| Series: | Inorganics |
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| Online Access: | https://www.mdpi.com/2304-6740/13/7/213 |
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| author | Maida Katherine Triviño-Rojas Santiago José Jiménez-Lopez Richard D’Vries Alberto Aragón-Muriel Dorian Polo-Cerón |
| author_facet | Maida Katherine Triviño-Rojas Santiago José Jiménez-Lopez Richard D’Vries Alberto Aragón-Muriel Dorian Polo-Cerón |
| author_sort | Maida Katherine Triviño-Rojas |
| collection | DOAJ |
| description | Their demonstrable bioactive characteristics, coupled with their wide structural diversity and coordination versatility, render Schiff bases and their coordination complexes biologically active compounds demonstrating outstanding properties. This research describes the synthesis and characterization of new Cu(II) and Ni(II) complexes with an NNO-donor hydrazone ligand (HL). The crystal structure of the HL ligand was determined through single-crystal X-ray diffraction studies. The in vitro antibacterial activities of the HL ligand and its metal(II) complexes against Gram-positive and Gram-negative bacteria demonstrated that the metal(II) complexes displayed greater antimicrobial activities compared to the free Schiff base ligand. Furthermore, the interaction of the ligand and the complexes with calf thymus DNA (CT-DNA) was explored through electronic absorption and viscosity measurements, suggesting intercalation as the most likely mode of binding. The compounds promoted oxidative DNA cleavage, as demonstrated by the strand breaks of the pmChery plasmid under oxidative stress conditions. Finally, fluorescence spectroscopy also revealed the strong binding affinity of these compounds for bovine serum albumin (BSA). |
| format | Article |
| id | doaj-art-b82d9b189f6d4d49b4e8c20fb1b9f1a0 |
| institution | DOAJ |
| issn | 2304-6740 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Inorganics |
| spelling | doaj-art-b82d9b189f6d4d49b4e8c20fb1b9f1a02025-08-20T02:45:56ZengMDPI AGInorganics2304-67402025-06-0113721310.3390/inorganics13070213<i>In Vitro</i> Antibacterial Activities and Calf Thymus DNA–Bovine Serum Albumin Interactions of Tridentate NNO Hydrazone Schiff Base–Metal ComplexesMaida Katherine Triviño-Rojas0Santiago José Jiménez-Lopez1Richard D’Vries2Alberto Aragón-Muriel3Dorian Polo-Cerón4Laboratorio de Investigación en Catálisis y Procesos (LICAP), Facultad de Ciencias Naturales y Exactas, Departamento de Química, Universidad del Valle, Cali 760001, ColombiaLaboratorio de Investigación en Catálisis y Procesos (LICAP), Facultad de Ciencias Naturales y Exactas, Departamento de Química, Universidad del Valle, Cali 760001, ColombiaGrupo de Investigación en Química de Productos Naturales, Departamento de Química, Facultad de Ciencias Naturales, Exactas y de la Educación, Universidad del Cauca, Popayán 190003, ColombiaGrupo de Investigaciones Bioquímicas (GIB), Departamento de Química, Universidad del Magdalena, Santa Marta 470004, ColombiaLaboratorio de Investigación en Catálisis y Procesos (LICAP), Facultad de Ciencias Naturales y Exactas, Departamento de Química, Universidad del Valle, Cali 760001, ColombiaTheir demonstrable bioactive characteristics, coupled with their wide structural diversity and coordination versatility, render Schiff bases and their coordination complexes biologically active compounds demonstrating outstanding properties. This research describes the synthesis and characterization of new Cu(II) and Ni(II) complexes with an NNO-donor hydrazone ligand (HL). The crystal structure of the HL ligand was determined through single-crystal X-ray diffraction studies. The in vitro antibacterial activities of the HL ligand and its metal(II) complexes against Gram-positive and Gram-negative bacteria demonstrated that the metal(II) complexes displayed greater antimicrobial activities compared to the free Schiff base ligand. Furthermore, the interaction of the ligand and the complexes with calf thymus DNA (CT-DNA) was explored through electronic absorption and viscosity measurements, suggesting intercalation as the most likely mode of binding. The compounds promoted oxidative DNA cleavage, as demonstrated by the strand breaks of the pmChery plasmid under oxidative stress conditions. Finally, fluorescence spectroscopy also revealed the strong binding affinity of these compounds for bovine serum albumin (BSA).https://www.mdpi.com/2304-6740/13/7/213hydrazoneantibacterial activitySchiff basesNNO chelatemetal complexesDNA interaction |
| spellingShingle | Maida Katherine Triviño-Rojas Santiago José Jiménez-Lopez Richard D’Vries Alberto Aragón-Muriel Dorian Polo-Cerón <i>In Vitro</i> Antibacterial Activities and Calf Thymus DNA–Bovine Serum Albumin Interactions of Tridentate NNO Hydrazone Schiff Base–Metal Complexes Inorganics hydrazone antibacterial activity Schiff bases NNO chelate metal complexes DNA interaction |
| title | <i>In Vitro</i> Antibacterial Activities and Calf Thymus DNA–Bovine Serum Albumin Interactions of Tridentate NNO Hydrazone Schiff Base–Metal Complexes |
| title_full | <i>In Vitro</i> Antibacterial Activities and Calf Thymus DNA–Bovine Serum Albumin Interactions of Tridentate NNO Hydrazone Schiff Base–Metal Complexes |
| title_fullStr | <i>In Vitro</i> Antibacterial Activities and Calf Thymus DNA–Bovine Serum Albumin Interactions of Tridentate NNO Hydrazone Schiff Base–Metal Complexes |
| title_full_unstemmed | <i>In Vitro</i> Antibacterial Activities and Calf Thymus DNA–Bovine Serum Albumin Interactions of Tridentate NNO Hydrazone Schiff Base–Metal Complexes |
| title_short | <i>In Vitro</i> Antibacterial Activities and Calf Thymus DNA–Bovine Serum Albumin Interactions of Tridentate NNO Hydrazone Schiff Base–Metal Complexes |
| title_sort | i in vitro i antibacterial activities and calf thymus dna bovine serum albumin interactions of tridentate nno hydrazone schiff base metal complexes |
| topic | hydrazone antibacterial activity Schiff bases NNO chelate metal complexes DNA interaction |
| url | https://www.mdpi.com/2304-6740/13/7/213 |
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