A Fluorescent Probe for Imaging and Treating S-Nitrosation Stress in OGD/R Cells
Protein S-nitrosation, a redox post-translational modification elicited by nitric oxide (NO), is essential for modulating diverse protein functions and signaling pathways. Dysregulation of S-nitrosation is implicated in various pathological processes, including oxygen-glucose deprivation/reperfusion...
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2025-03-01
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| Series: | Antioxidants |
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| author | Hui Ye Chen Zhang Lerong Li Cunrui Li Jiayue Yu Duorui Ji Zhuangzhuang Liang Jianbing Wu Zhangjian Huang |
| author_facet | Hui Ye Chen Zhang Lerong Li Cunrui Li Jiayue Yu Duorui Ji Zhuangzhuang Liang Jianbing Wu Zhangjian Huang |
| author_sort | Hui Ye |
| collection | DOAJ |
| description | Protein S-nitrosation, a redox post-translational modification elicited by nitric oxide (NO), is essential for modulating diverse protein functions and signaling pathways. Dysregulation of S-nitrosation is implicated in various pathological processes, including oxygen-glucose deprivation/reperfusion (OGD/R) injury, a widely used model for ischemia-reperfusion diseases. The dynamic changes in S-nitrosothiols (SNOs) during ischemia-reperfusion highlight the need for theranostic strategies to monitor and modulate SNO levels based on pathological progression. However, to date, no theranostic strategies have been reported for addressing dysregulated SNO in disease models, particularly in OGD/R conditions. Here, we report the development of a selective probe <b>P-EHC</b>, which could specifically react with SNOs to release <b>EHC</b>, not only exhibiting turn-on fluorescence with high quantum yield and good water solubility but also demonstrating macrophage migration inhibitory factor (MIF) inhibitory activity. In an OGD/R model of SH-SY5Y cells, we observed elevated SNO levels by using live-cell confocal imaging. Treatment of <b>P-EHC</b> significantly reduced intracellular reactive oxygen species (ROS), lowered total NO<sub>x</sub> species, and improved cell viability in the OGD/R model. In summary, the simplicity and versatility of <b>P-EHC</b> suggest its broad applicability for monitoring SNO in various biological models and therapeutic contexts, particularly in ischemia-reperfusion diseases. |
| format | Article |
| id | doaj-art-b8223edc71f7419996461a1f0bd5c8bb |
| institution | OA Journals |
| issn | 2076-3921 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
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| series | Antioxidants |
| spelling | doaj-art-b8223edc71f7419996461a1f0bd5c8bb2025-08-20T02:11:21ZengMDPI AGAntioxidants2076-39212025-03-0114331110.3390/antiox14030311A Fluorescent Probe for Imaging and Treating S-Nitrosation Stress in OGD/R CellsHui Ye0Chen Zhang1Lerong Li2Cunrui Li3Jiayue Yu4Duorui Ji5Zhuangzhuang Liang6Jianbing Wu7Zhangjian Huang8State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 211198, ChinaState Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 211198, ChinaState Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 211198, ChinaState Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 211198, ChinaState Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 211198, ChinaState Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 211198, ChinaState Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 211198, ChinaState Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 211198, ChinaState Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 211198, ChinaProtein S-nitrosation, a redox post-translational modification elicited by nitric oxide (NO), is essential for modulating diverse protein functions and signaling pathways. Dysregulation of S-nitrosation is implicated in various pathological processes, including oxygen-glucose deprivation/reperfusion (OGD/R) injury, a widely used model for ischemia-reperfusion diseases. The dynamic changes in S-nitrosothiols (SNOs) during ischemia-reperfusion highlight the need for theranostic strategies to monitor and modulate SNO levels based on pathological progression. However, to date, no theranostic strategies have been reported for addressing dysregulated SNO in disease models, particularly in OGD/R conditions. Here, we report the development of a selective probe <b>P-EHC</b>, which could specifically react with SNOs to release <b>EHC</b>, not only exhibiting turn-on fluorescence with high quantum yield and good water solubility but also demonstrating macrophage migration inhibitory factor (MIF) inhibitory activity. In an OGD/R model of SH-SY5Y cells, we observed elevated SNO levels by using live-cell confocal imaging. Treatment of <b>P-EHC</b> significantly reduced intracellular reactive oxygen species (ROS), lowered total NO<sub>x</sub> species, and improved cell viability in the OGD/R model. In summary, the simplicity and versatility of <b>P-EHC</b> suggest its broad applicability for monitoring SNO in various biological models and therapeutic contexts, particularly in ischemia-reperfusion diseases.https://www.mdpi.com/2076-3921/14/3/311S-nitrosationnitric oxideS-nitrosylationtheranosticOGD/Rimaging |
| spellingShingle | Hui Ye Chen Zhang Lerong Li Cunrui Li Jiayue Yu Duorui Ji Zhuangzhuang Liang Jianbing Wu Zhangjian Huang A Fluorescent Probe for Imaging and Treating S-Nitrosation Stress in OGD/R Cells Antioxidants S-nitrosation nitric oxide S-nitrosylation theranostic OGD/R imaging |
| title | A Fluorescent Probe for Imaging and Treating S-Nitrosation Stress in OGD/R Cells |
| title_full | A Fluorescent Probe for Imaging and Treating S-Nitrosation Stress in OGD/R Cells |
| title_fullStr | A Fluorescent Probe for Imaging and Treating S-Nitrosation Stress in OGD/R Cells |
| title_full_unstemmed | A Fluorescent Probe for Imaging and Treating S-Nitrosation Stress in OGD/R Cells |
| title_short | A Fluorescent Probe for Imaging and Treating S-Nitrosation Stress in OGD/R Cells |
| title_sort | fluorescent probe for imaging and treating s nitrosation stress in ogd r cells |
| topic | S-nitrosation nitric oxide S-nitrosylation theranostic OGD/R imaging |
| url | https://www.mdpi.com/2076-3921/14/3/311 |
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