Liquid crystal template-assisted electrodeposition of nanoporous nickel microspheres: A sensitive electrochemical sensing platform for electrocatalytic oxidation and quantitative determination of l-carnitine
l-carnitine (LC) is a vital constituent of mammalian tissues, has a significant role in the function of different biological systems, and its determination is of great interest. Electrochemical sensing using nickelaceous nanostructured electrocatalysts offers potential interests and advantages. In t...
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Elsevier
2025-02-01
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| Series: | Sensing and Bio-Sensing Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214180425000091 |
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| author | R. Dehdari Vais M. Negahdary S.R. Rasouli Nasrabadi A. Rahi S.A. Dastgheib H. Heli |
| author_facet | R. Dehdari Vais M. Negahdary S.R. Rasouli Nasrabadi A. Rahi S.A. Dastgheib H. Heli |
| author_sort | R. Dehdari Vais |
| collection | DOAJ |
| description | l-carnitine (LC) is a vital constituent of mammalian tissues, has a significant role in the function of different biological systems, and its determination is of great interest. Electrochemical sensing using nickelaceous nanostructured electrocatalysts offers potential interests and advantages. In the present study, nanoporous nickel microspheres (NNiMs) were electrodeposited and then utilized as an electrode modifier for the electrocatalytic oxidation and determination of LC. NNiMs were electrodeposited on a nickel substrate from a liquid crystal medium containing nickel (II) chloride, and Triton X-100 under a potentiostatic condition. The surface morphological characterization of the obtained nanoporous microspheres was followed by field emission scanning electron microscopy. NNiMs were transformed into the corresponding oxides by applying consecutive potential cycles in an alkaline medium, and the electron transfer coefficient and apparent charge transfer rate constant of the redox species present on the modified electrode surface were calculated as 0.59 and 0.54 s−1, respectively. Cyclic voltammetry, chronoamperometry, and steady-state polarization measurements were used for assessing the electrocatalytic oxidation mechanism and kinetics of LC on the NNiMs surface. Based on these measurements, an LC diffusion coefficient of 4.1 × 10−6 cm2 s−1, a catalytic rate constant of 4.0 × 104 cm3 mol−1 s−1, and an electron transfer coefficient of 0.41 were achieved. The proposed sensor was then employed as a sensitive amperometric sensor for determination of LC with a linear dynamic range of 25 to 217 μmol L−1, a calibration sensitivity of 67.85 mA L mol−1, and a detection limit of 3.2 μmol L−1. The designed sensing platform depicted a stable and reproducible response and insignificant interference from the common species found in the biological fluids and pharmaceutical formulations. Taking all these features together, the developed determination method can be satisfactorily exploited as a simple and quick tool for direct analysis of LC in pharmaceutical oral solutions and human serum samples. |
| format | Article |
| id | doaj-art-b81f34f00ece4b8888f42a212a147df2 |
| institution | Kabale University |
| issn | 2214-1804 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Sensing and Bio-Sensing Research |
| spelling | doaj-art-b81f34f00ece4b8888f42a212a147df22025-01-27T04:21:57ZengElsevierSensing and Bio-Sensing Research2214-18042025-02-0147100743Liquid crystal template-assisted electrodeposition of nanoporous nickel microspheres: A sensitive electrochemical sensing platform for electrocatalytic oxidation and quantitative determination of l-carnitineR. Dehdari Vais0M. Negahdary1S.R. Rasouli Nasrabadi2A. Rahi3S.A. Dastgheib4H. Heli5Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Biomedical Engineering, Texas A&M University, 600 Discovery Drive, College Station, TX 77840-3006, USA; Center for Remote Health Technologies & Systems, Texas A&M Engineering Experiment Station, 600 Discovery Drive, College Station, TX 77840-3006, USADepartment of Chemistry, K.N. Toosi University of Technology, Tehran, IranPathology and Stem cell Research Center, Kerman University of Medical Sciences, Kerman, IranNanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Medical Genetics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranNanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Corresponding author.l-carnitine (LC) is a vital constituent of mammalian tissues, has a significant role in the function of different biological systems, and its determination is of great interest. Electrochemical sensing using nickelaceous nanostructured electrocatalysts offers potential interests and advantages. In the present study, nanoporous nickel microspheres (NNiMs) were electrodeposited and then utilized as an electrode modifier for the electrocatalytic oxidation and determination of LC. NNiMs were electrodeposited on a nickel substrate from a liquid crystal medium containing nickel (II) chloride, and Triton X-100 under a potentiostatic condition. The surface morphological characterization of the obtained nanoporous microspheres was followed by field emission scanning electron microscopy. NNiMs were transformed into the corresponding oxides by applying consecutive potential cycles in an alkaline medium, and the electron transfer coefficient and apparent charge transfer rate constant of the redox species present on the modified electrode surface were calculated as 0.59 and 0.54 s−1, respectively. Cyclic voltammetry, chronoamperometry, and steady-state polarization measurements were used for assessing the electrocatalytic oxidation mechanism and kinetics of LC on the NNiMs surface. Based on these measurements, an LC diffusion coefficient of 4.1 × 10−6 cm2 s−1, a catalytic rate constant of 4.0 × 104 cm3 mol−1 s−1, and an electron transfer coefficient of 0.41 were achieved. The proposed sensor was then employed as a sensitive amperometric sensor for determination of LC with a linear dynamic range of 25 to 217 μmol L−1, a calibration sensitivity of 67.85 mA L mol−1, and a detection limit of 3.2 μmol L−1. The designed sensing platform depicted a stable and reproducible response and insignificant interference from the common species found in the biological fluids and pharmaceutical formulations. Taking all these features together, the developed determination method can be satisfactorily exploited as a simple and quick tool for direct analysis of LC in pharmaceutical oral solutions and human serum samples.http://www.sciencedirect.com/science/article/pii/S2214180425000091CarnitineElectroanalysisElectrocatalysisModified electrodeNanopore |
| spellingShingle | R. Dehdari Vais M. Negahdary S.R. Rasouli Nasrabadi A. Rahi S.A. Dastgheib H. Heli Liquid crystal template-assisted electrodeposition of nanoporous nickel microspheres: A sensitive electrochemical sensing platform for electrocatalytic oxidation and quantitative determination of l-carnitine Sensing and Bio-Sensing Research Carnitine Electroanalysis Electrocatalysis Modified electrode Nanopore |
| title | Liquid crystal template-assisted electrodeposition of nanoporous nickel microspheres: A sensitive electrochemical sensing platform for electrocatalytic oxidation and quantitative determination of l-carnitine |
| title_full | Liquid crystal template-assisted electrodeposition of nanoporous nickel microspheres: A sensitive electrochemical sensing platform for electrocatalytic oxidation and quantitative determination of l-carnitine |
| title_fullStr | Liquid crystal template-assisted electrodeposition of nanoporous nickel microspheres: A sensitive electrochemical sensing platform for electrocatalytic oxidation and quantitative determination of l-carnitine |
| title_full_unstemmed | Liquid crystal template-assisted electrodeposition of nanoporous nickel microspheres: A sensitive electrochemical sensing platform for electrocatalytic oxidation and quantitative determination of l-carnitine |
| title_short | Liquid crystal template-assisted electrodeposition of nanoporous nickel microspheres: A sensitive electrochemical sensing platform for electrocatalytic oxidation and quantitative determination of l-carnitine |
| title_sort | liquid crystal template assisted electrodeposition of nanoporous nickel microspheres a sensitive electrochemical sensing platform for electrocatalytic oxidation and quantitative determination of l carnitine |
| topic | Carnitine Electroanalysis Electrocatalysis Modified electrode Nanopore |
| url | http://www.sciencedirect.com/science/article/pii/S2214180425000091 |
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