Rapid and high-throughput analysis of 5-hydroxymethylfurfural in honey by MALDI-MS with in-situ tetra (4-aminophenyl)-porphyrin derivatization
Abstract 5-Hydroxymethylfurfural (HMF), a product of the Maillard reaction and caramelization in honey, serves as a crucial marker of food quality and thermal processing, underscoring the importance of regulatory surveillance for consumer safety. This study introduces a method for rapid, high throug...
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| Format: | Article |
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Nature Portfolio
2025-05-01
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| Series: | npj Science of Food |
| Online Access: | https://doi.org/10.1038/s41538-025-00431-w |
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| author | Zhi Sun Fangfang Wang Peipei Zhou Di Chen Lihua Zuo |
| author_facet | Zhi Sun Fangfang Wang Peipei Zhou Di Chen Lihua Zuo |
| author_sort | Zhi Sun |
| collection | DOAJ |
| description | Abstract 5-Hydroxymethylfurfural (HMF), a product of the Maillard reaction and caramelization in honey, serves as a crucial marker of food quality and thermal processing, underscoring the importance of regulatory surveillance for consumer safety. This study introduces a method for rapid, high throughput and eco-friendly detection of HMF, using derivatization in matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). 5,10,15,20-Tetrakis-(4-aminophenyl)-porphyrin was employed for in-situ derivatization of aldehydes after extraction by ethyl acetate, enhancing detection sensitivity by shifting low-molecular-weight aldehydes to higher regions. The calibration curves for HMF determination exhibited outstanding linearity. The detection limit reached 0.347 mg/kg with good accuracy and precision. Variations in the content of HMF in honey during heating were also assessed, revealing the increased formation of HMF under high temperature and prolonged heating. The method’s proficiency was evidenced through the detection of various aldehydes. This method represents an advance in rapid analysis for food safety, aligning with green chemistry principles. |
| format | Article |
| id | doaj-art-b81ccd73e2d1468db756fa660b6ff343 |
| institution | Kabale University |
| issn | 2396-8370 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Science of Food |
| spelling | doaj-art-b81ccd73e2d1468db756fa660b6ff3432025-08-20T03:53:14ZengNature Portfolionpj Science of Food2396-83702025-05-019111010.1038/s41538-025-00431-wRapid and high-throughput analysis of 5-hydroxymethylfurfural in honey by MALDI-MS with in-situ tetra (4-aminophenyl)-porphyrin derivatizationZhi Sun0Fangfang Wang1Peipei Zhou2Di Chen3Lihua Zuo4Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou UniversityDepartment of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou UniversityDepartment of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou UniversityZhengzhou Research Base, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, School of Pharmaceutical Sciences, Zhengzhou UniversityDepartment of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou UniversityAbstract 5-Hydroxymethylfurfural (HMF), a product of the Maillard reaction and caramelization in honey, serves as a crucial marker of food quality and thermal processing, underscoring the importance of regulatory surveillance for consumer safety. This study introduces a method for rapid, high throughput and eco-friendly detection of HMF, using derivatization in matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). 5,10,15,20-Tetrakis-(4-aminophenyl)-porphyrin was employed for in-situ derivatization of aldehydes after extraction by ethyl acetate, enhancing detection sensitivity by shifting low-molecular-weight aldehydes to higher regions. The calibration curves for HMF determination exhibited outstanding linearity. The detection limit reached 0.347 mg/kg with good accuracy and precision. Variations in the content of HMF in honey during heating were also assessed, revealing the increased formation of HMF under high temperature and prolonged heating. The method’s proficiency was evidenced through the detection of various aldehydes. This method represents an advance in rapid analysis for food safety, aligning with green chemistry principles.https://doi.org/10.1038/s41538-025-00431-w |
| spellingShingle | Zhi Sun Fangfang Wang Peipei Zhou Di Chen Lihua Zuo Rapid and high-throughput analysis of 5-hydroxymethylfurfural in honey by MALDI-MS with in-situ tetra (4-aminophenyl)-porphyrin derivatization npj Science of Food |
| title | Rapid and high-throughput analysis of 5-hydroxymethylfurfural in honey by MALDI-MS with in-situ tetra (4-aminophenyl)-porphyrin derivatization |
| title_full | Rapid and high-throughput analysis of 5-hydroxymethylfurfural in honey by MALDI-MS with in-situ tetra (4-aminophenyl)-porphyrin derivatization |
| title_fullStr | Rapid and high-throughput analysis of 5-hydroxymethylfurfural in honey by MALDI-MS with in-situ tetra (4-aminophenyl)-porphyrin derivatization |
| title_full_unstemmed | Rapid and high-throughput analysis of 5-hydroxymethylfurfural in honey by MALDI-MS with in-situ tetra (4-aminophenyl)-porphyrin derivatization |
| title_short | Rapid and high-throughput analysis of 5-hydroxymethylfurfural in honey by MALDI-MS with in-situ tetra (4-aminophenyl)-porphyrin derivatization |
| title_sort | rapid and high throughput analysis of 5 hydroxymethylfurfural in honey by maldi ms with in situ tetra 4 aminophenyl porphyrin derivatization |
| url | https://doi.org/10.1038/s41538-025-00431-w |
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