SCFAs induce mouse neutrophil chemotaxis through the GPR43 receptor.
Short chain fatty acids (SCFAs) have recently attracted attention as potential mediators of the effects of gut microbiota on intestinal inflammation. Some of these effects have been suggested to occur through the direct actions of SCFAs on the GPR43 receptor in neutrophils, though the precise role o...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0021205&type=printable |
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| author | Marco A R Vinolo G John Ferguson Suhasini Kulkarni George Damoulakis Karen Anderson Mohammad Bohlooly-Y Len Stephens Phillip T Hawkins Rui Curi |
| author_facet | Marco A R Vinolo G John Ferguson Suhasini Kulkarni George Damoulakis Karen Anderson Mohammad Bohlooly-Y Len Stephens Phillip T Hawkins Rui Curi |
| author_sort | Marco A R Vinolo |
| collection | DOAJ |
| description | Short chain fatty acids (SCFAs) have recently attracted attention as potential mediators of the effects of gut microbiota on intestinal inflammation. Some of these effects have been suggested to occur through the direct actions of SCFAs on the GPR43 receptor in neutrophils, though the precise role of this receptor in neutrophil activation is still unclear. We show that mouse bone marrow derived neutrophils (BMNs) can chemotax effectively through polycarbonate filters towards a source of acetate, propionate or butyrate. Moreover, we show that BMNs move with good speed and directionality towards a source of propionate in an EZ-Taxiscan chamber coated with fibrinogen. These effects of SCFAs were mimicked by low concentrations of the synthetic GPR43 agonist phenylacetamide-1 and were abolished in GPR43(-/-) BMNs. SCFAs and phenylacetamide-1 also elicited GPR43-dependent activation of PKB, p38 and ERK and these responses were sensitive to pertussis toxin, indicating a role for Gi proteins. Phenylacetamide-1 also elicited rapid and transient activation of Rac1/2 GTPases and phosphorylation of ribosomal protein S6. Genetic and pharmacological intervention identified important roles for PI3Kγ, Rac2, p38 and ERK, but not mTOR, in GPR43-dependent chemotaxis. These results identify GPR43 as a bona fide chemotactic receptor for neutrophils in vitro and start to define important elements in its signal transduction pathways. |
| format | Article |
| id | doaj-art-b81bd6ce141845e09edfc122af8ac149 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-b81bd6ce141845e09edfc122af8ac1492025-08-20T03:09:49ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0166e2120510.1371/journal.pone.0021205SCFAs induce mouse neutrophil chemotaxis through the GPR43 receptor.Marco A R VinoloG John FergusonSuhasini KulkarniGeorge DamoulakisKaren AndersonMohammad Bohlooly-YLen StephensPhillip T HawkinsRui CuriShort chain fatty acids (SCFAs) have recently attracted attention as potential mediators of the effects of gut microbiota on intestinal inflammation. Some of these effects have been suggested to occur through the direct actions of SCFAs on the GPR43 receptor in neutrophils, though the precise role of this receptor in neutrophil activation is still unclear. We show that mouse bone marrow derived neutrophils (BMNs) can chemotax effectively through polycarbonate filters towards a source of acetate, propionate or butyrate. Moreover, we show that BMNs move with good speed and directionality towards a source of propionate in an EZ-Taxiscan chamber coated with fibrinogen. These effects of SCFAs were mimicked by low concentrations of the synthetic GPR43 agonist phenylacetamide-1 and were abolished in GPR43(-/-) BMNs. SCFAs and phenylacetamide-1 also elicited GPR43-dependent activation of PKB, p38 and ERK and these responses were sensitive to pertussis toxin, indicating a role for Gi proteins. Phenylacetamide-1 also elicited rapid and transient activation of Rac1/2 GTPases and phosphorylation of ribosomal protein S6. Genetic and pharmacological intervention identified important roles for PI3Kγ, Rac2, p38 and ERK, but not mTOR, in GPR43-dependent chemotaxis. These results identify GPR43 as a bona fide chemotactic receptor for neutrophils in vitro and start to define important elements in its signal transduction pathways.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0021205&type=printable |
| spellingShingle | Marco A R Vinolo G John Ferguson Suhasini Kulkarni George Damoulakis Karen Anderson Mohammad Bohlooly-Y Len Stephens Phillip T Hawkins Rui Curi SCFAs induce mouse neutrophil chemotaxis through the GPR43 receptor. PLoS ONE |
| title | SCFAs induce mouse neutrophil chemotaxis through the GPR43 receptor. |
| title_full | SCFAs induce mouse neutrophil chemotaxis through the GPR43 receptor. |
| title_fullStr | SCFAs induce mouse neutrophil chemotaxis through the GPR43 receptor. |
| title_full_unstemmed | SCFAs induce mouse neutrophil chemotaxis through the GPR43 receptor. |
| title_short | SCFAs induce mouse neutrophil chemotaxis through the GPR43 receptor. |
| title_sort | scfas induce mouse neutrophil chemotaxis through the gpr43 receptor |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0021205&type=printable |
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