Effects of Osseointegration by Bone Morphogenetic Protein-2 on Titanium Implants In Vitro and In Vivo
This study designed a biomimetic implant for reducing healing time and achieving early osseointegration to create an active surface. Bone morphogenetic protein-2 (BMP-2) is a strong regulator protein in osteogenic pathways. Due to hardly maintaining BMP-2 biological function and specificity, BMP-2 e...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Bioinorganic Chemistry and Applications |
| Online Access: | http://dx.doi.org/10.1155/2016/3837679 |
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| _version_ | 1832550770511183872 |
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| author | Fu-Yuan Teng Wen-Cheng Chen Yin-Lai Wang Chun-Cheng Hung Chun-Chieh Tseng |
| author_facet | Fu-Yuan Teng Wen-Cheng Chen Yin-Lai Wang Chun-Cheng Hung Chun-Chieh Tseng |
| author_sort | Fu-Yuan Teng |
| collection | DOAJ |
| description | This study designed a biomimetic implant for reducing healing time and achieving early osseointegration to create an active surface. Bone morphogenetic protein-2 (BMP-2) is a strong regulator protein in osteogenic pathways. Due to hardly maintaining BMP-2 biological function and specificity, BMP-2 efficient delivery on implant surfaces is the main challenge for the clinic application. In this study, a novel method for synthesizing functionalized silane film for superior modification with BMP-2 on titanium surfaces is proposed. Three groups were compared with and without BMP-2 on modified titanium surfaces in vitro and in vivo: mechanical grinding; electrochemical modification through potentiostatic anodization (ECH); and sandblasting, alkali heating, and etching (SMART). Cell tests indicated that the ECH and SMART groups with BMP-2 markedly promoted D1 cell activity and differentiation compared with the groups without BMP-2. Moreover, the SMART group with a BMP-2 surface markedly promoted early alkaline phosphatase expression in the D1 cells compared with the other surface groups. Compared with these groups in vivo, SMART silaning with BMP-2 showed superior bone quality and created contact areas between implant and surrounding bones. The SMART group with BMP-2 could promote cell mineralization in vitro and osseointegration in vivo, indicating potential clinical use. |
| format | Article |
| id | doaj-art-b8093d4dac354f8990c5075955d7be0e |
| institution | Kabale University |
| issn | 1565-3633 1687-479X |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioinorganic Chemistry and Applications |
| spelling | doaj-art-b8093d4dac354f8990c5075955d7be0e2025-02-03T06:05:57ZengWileyBioinorganic Chemistry and Applications1565-36331687-479X2016-01-01201610.1155/2016/38376793837679Effects of Osseointegration by Bone Morphogenetic Protein-2 on Titanium Implants In Vitro and In VivoFu-Yuan Teng0Wen-Cheng Chen1Yin-Lai Wang2Chun-Cheng Hung3Chun-Chieh Tseng4Department of Dentistry, Kaohsiung Armed Forces General Hospital, Kaohsiung, TaiwanAdvanced Medical Devices and Composites Laboratory, Department of Fiber and Composite Materials, College of Engineering, Feng Chia University, Taichung, TaiwanDepartment of Dentistry, Kaohsiung Armed Forces General Hospital, Kaohsiung, TaiwanSchool of Dentistry, College of Dental Medicine, Kaohsiung Medical University, Department of Prosthodontics, Kaohsiung Medical University Hospital, No. 100 Tzyou 1st Road, Kaohsiung 80708, TaiwanMetal Industries Research & Development Centre, 3F, No. 88 Luke 5th Road, Luzhu District, Kaohsiung 82151, TaiwanThis study designed a biomimetic implant for reducing healing time and achieving early osseointegration to create an active surface. Bone morphogenetic protein-2 (BMP-2) is a strong regulator protein in osteogenic pathways. Due to hardly maintaining BMP-2 biological function and specificity, BMP-2 efficient delivery on implant surfaces is the main challenge for the clinic application. In this study, a novel method for synthesizing functionalized silane film for superior modification with BMP-2 on titanium surfaces is proposed. Three groups were compared with and without BMP-2 on modified titanium surfaces in vitro and in vivo: mechanical grinding; electrochemical modification through potentiostatic anodization (ECH); and sandblasting, alkali heating, and etching (SMART). Cell tests indicated that the ECH and SMART groups with BMP-2 markedly promoted D1 cell activity and differentiation compared with the groups without BMP-2. Moreover, the SMART group with a BMP-2 surface markedly promoted early alkaline phosphatase expression in the D1 cells compared with the other surface groups. Compared with these groups in vivo, SMART silaning with BMP-2 showed superior bone quality and created contact areas between implant and surrounding bones. The SMART group with BMP-2 could promote cell mineralization in vitro and osseointegration in vivo, indicating potential clinical use.http://dx.doi.org/10.1155/2016/3837679 |
| spellingShingle | Fu-Yuan Teng Wen-Cheng Chen Yin-Lai Wang Chun-Cheng Hung Chun-Chieh Tseng Effects of Osseointegration by Bone Morphogenetic Protein-2 on Titanium Implants In Vitro and In Vivo Bioinorganic Chemistry and Applications |
| title | Effects of Osseointegration by Bone Morphogenetic Protein-2 on Titanium Implants In Vitro and In Vivo |
| title_full | Effects of Osseointegration by Bone Morphogenetic Protein-2 on Titanium Implants In Vitro and In Vivo |
| title_fullStr | Effects of Osseointegration by Bone Morphogenetic Protein-2 on Titanium Implants In Vitro and In Vivo |
| title_full_unstemmed | Effects of Osseointegration by Bone Morphogenetic Protein-2 on Titanium Implants In Vitro and In Vivo |
| title_short | Effects of Osseointegration by Bone Morphogenetic Protein-2 on Titanium Implants In Vitro and In Vivo |
| title_sort | effects of osseointegration by bone morphogenetic protein 2 on titanium implants in vitro and in vivo |
| url | http://dx.doi.org/10.1155/2016/3837679 |
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