Construction and optimization of a versatile bioFET platform for detecting nucleic acids and protein using the biotin–neutravidin affinity system
A biosensor based on a field-effect transistor (bioFET) is a promising system for point-of-care testing due to its portability and high sensitivity. A bioFET uses a linker platform to connect capture molecules to the FET, which detects electrical changes when targets bind to the capture molecules. T...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | Sensing and Bio-Sensing Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214180425000443 |
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| author | Jiunn-Tyng Yeh Lian-Chin Wang Chi-Pei Weng Chang-Fu Kuo |
| author_facet | Jiunn-Tyng Yeh Lian-Chin Wang Chi-Pei Weng Chang-Fu Kuo |
| author_sort | Jiunn-Tyng Yeh |
| collection | DOAJ |
| description | A biosensor based on a field-effect transistor (bioFET) is a promising system for point-of-care testing due to its portability and high sensitivity. A bioFET uses a linker platform to connect capture molecules to the FET, which detects electrical changes when targets bind to the capture molecules. This study presents the development and optimization of a versatile, highly sensitive bioFET platform utilizing a biotin–neutravidin affinity system capable of detecting both DNA and proteins. The bioFET was constructed using an extended-gate field-effect transistor (EGFET) with an aluminum gate functionalized with (3-aminopropyl)triethoxysilane (APTES)–biotin–neutravidin. We optimized the concentrations and molar ratio of biotin and neutravidin using fluorescence imaging and biosensing benchmarks. We found that 0.1 μg/mL biotin paired with 30 μg/mL neutravidin, approximately 1:1 in molarity, achieved optimal sensitivity. The optimized bioFET system demonstrated good sensitivity, with lower detection limits of 3.5 copies for Escherichia coli genomic DNA and 0.3 fg/mL for p-Tau217, an Alzheimer's disease biomarker. In summary, this study establishes the ideal conditions for constructing a bioFET system using an APTES–biotin–neutravidin linker capable of detecting DNA and proteins, with potential for convenient and sensitive point-of-care diagnostics. |
| format | Article |
| id | doaj-art-b80153f735d348bdb8e5b02bcd5e1d6d |
| institution | DOAJ |
| issn | 2214-1804 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Sensing and Bio-Sensing Research |
| spelling | doaj-art-b80153f735d348bdb8e5b02bcd5e1d6d2025-08-20T03:20:03ZengElsevierSensing and Bio-Sensing Research2214-18042025-06-014810077810.1016/j.sbsr.2025.100778Construction and optimization of a versatile bioFET platform for detecting nucleic acids and protein using the biotin–neutravidin affinity systemJiunn-Tyng Yeh0Lian-Chin Wang1Chi-Pei Weng2Chang-Fu Kuo3Novascope Diagnostics INC, Taipei, Taiwan; Center for Artificial Intelligence in Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan, TaiwanNovascope Diagnostics INC, Taipei, TaiwanNovascope Diagnostics INC, Taipei, TaiwanNovascope Diagnostics INC, Taipei, Taiwan; Center for Artificial Intelligence in Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan; Division of Rheumatology, Allergy and Immunology, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan; Department of Internal Medicine, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Division of Rheumatology, Orthopaedics and Dermatology, School of Medicine, University of Nottingham, Nottingham, UK; Corresponding author at: Research Building 3rd Floor AI Core Lab, No. 15 Wenhua 1st Rd., Guishan Dist., Taoyuan 333, Taiwan.A biosensor based on a field-effect transistor (bioFET) is a promising system for point-of-care testing due to its portability and high sensitivity. A bioFET uses a linker platform to connect capture molecules to the FET, which detects electrical changes when targets bind to the capture molecules. This study presents the development and optimization of a versatile, highly sensitive bioFET platform utilizing a biotin–neutravidin affinity system capable of detecting both DNA and proteins. The bioFET was constructed using an extended-gate field-effect transistor (EGFET) with an aluminum gate functionalized with (3-aminopropyl)triethoxysilane (APTES)–biotin–neutravidin. We optimized the concentrations and molar ratio of biotin and neutravidin using fluorescence imaging and biosensing benchmarks. We found that 0.1 μg/mL biotin paired with 30 μg/mL neutravidin, approximately 1:1 in molarity, achieved optimal sensitivity. The optimized bioFET system demonstrated good sensitivity, with lower detection limits of 3.5 copies for Escherichia coli genomic DNA and 0.3 fg/mL for p-Tau217, an Alzheimer's disease biomarker. In summary, this study establishes the ideal conditions for constructing a bioFET system using an APTES–biotin–neutravidin linker capable of detecting DNA and proteins, with potential for convenient and sensitive point-of-care diagnostics.http://www.sciencedirect.com/science/article/pii/S2214180425000443EGFETbioFETBiotinNeutravidinBiosensor |
| spellingShingle | Jiunn-Tyng Yeh Lian-Chin Wang Chi-Pei Weng Chang-Fu Kuo Construction and optimization of a versatile bioFET platform for detecting nucleic acids and protein using the biotin–neutravidin affinity system Sensing and Bio-Sensing Research EGFET bioFET Biotin Neutravidin Biosensor |
| title | Construction and optimization of a versatile bioFET platform for detecting nucleic acids and protein using the biotin–neutravidin affinity system |
| title_full | Construction and optimization of a versatile bioFET platform for detecting nucleic acids and protein using the biotin–neutravidin affinity system |
| title_fullStr | Construction and optimization of a versatile bioFET platform for detecting nucleic acids and protein using the biotin–neutravidin affinity system |
| title_full_unstemmed | Construction and optimization of a versatile bioFET platform for detecting nucleic acids and protein using the biotin–neutravidin affinity system |
| title_short | Construction and optimization of a versatile bioFET platform for detecting nucleic acids and protein using the biotin–neutravidin affinity system |
| title_sort | construction and optimization of a versatile biofet platform for detecting nucleic acids and protein using the biotin neutravidin affinity system |
| topic | EGFET bioFET Biotin Neutravidin Biosensor |
| url | http://www.sciencedirect.com/science/article/pii/S2214180425000443 |
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