Getting under the skin of the menopausal hot flush: a protocol to examine skin function and structure in symptomatic postmenopausal women

IntroductionThe major pathophysiological symptom of the menopause affecting daily life is hot flushes, which are also associated with elevated cardiovascular disease risk. A hot flush is a sudden and intense heat sensation causing skin flushing and profuse sweating. Menopause-induced oestrogen defic...

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Main Authors: Kirsty A. Roberts, Abigail Doyle, Helen Jones, David A. Low
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-08-01
Series:Frontiers in Global Women's Health
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Online Access:https://www.frontiersin.org/articles/10.3389/fgwh.2025.1514960/full
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author Kirsty A. Roberts
Kirsty A. Roberts
Abigail Doyle
Helen Jones
Helen Jones
David A. Low
David A. Low
author_facet Kirsty A. Roberts
Kirsty A. Roberts
Abigail Doyle
Helen Jones
Helen Jones
David A. Low
David A. Low
author_sort Kirsty A. Roberts
collection DOAJ
description IntroductionThe major pathophysiological symptom of the menopause affecting daily life is hot flushes, which are also associated with elevated cardiovascular disease risk. A hot flush is a sudden and intense heat sensation causing skin flushing and profuse sweating. Menopause-induced oestrogen deficiency could increase the sensitivity of skin blood vessels and sweat glands in postmenopausal women, which could result in more frequent and larger increases in skin blood flow in postmenopausal women consistent with hot flushes. Furthermore, oestrogen withdrawal could also alter the structure of the skin blood vessels and/or sweat glands which may also contribute to hot flushes. This trial aims to examine the function and structure of skin blood vessels and sweat glands in premenopausal and postmenopausal women.Methods and analysisThis is a single-centre multi-cohort observational study. Participants will attend the laboratory at Liverpool John Moores University (LJMU) on two separate occasions, ∼7 days apart. Visit 1 will consist of anthropometry, a blood sample and assessment of post-ganglionic skin blood vessel and sweat gland responsiveness via cutaneous microdialysis. At visit 2, participants will return for a skin punch biopsy. A between groups statistical analysis of the pre- and postmenopausal cohorts will be conducted in a blinded manner.Ethics and disseminationThe trial was approved by the North West - Greater Manchester South Research Ethics Committee (22/NW/0300) in the UK. The study adheres to The Declaration of Helsinki and is being conducted in accordance with the UK Policy Framework for Health and Social Care Research.DiscussionIdentifying functional and/or structural changes in skin blood vessels or sweat glands in women with hot flushes would increase our understanding of their cause(s) and side effects, and help to design effective treatments, including interventions that can manipulate the activity of the skin blood vessels and/or sweat glands via pharmacological or non-pharmacological methods. Trial registration numbersNCT06222073.
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spelling doaj-art-b7ff9c98628c4414b69af3858011032a2025-08-20T03:18:27ZengFrontiers Media S.A.Frontiers in Global Women's Health2673-50592025-08-01610.3389/fgwh.2025.15149601514960Getting under the skin of the menopausal hot flush: a protocol to examine skin function and structure in symptomatic postmenopausal womenKirsty A. Roberts0Kirsty A. Roberts1Abigail Doyle2Helen Jones3Helen Jones4David A. Low5David A. Low6Research Institute for Sport and Exercise Science, Liverpool John Moores University, Liverpool, United KingdomLiverpool Centre for Cardiovascular Science at University of Liverpool, Liverpool John Moores University and Liverpool Heart & Chest Hospital, Liverpool, United KingdomResearch Institute for Sport and Exercise Science, Liverpool John Moores University, Liverpool, United KingdomResearch Institute for Sport and Exercise Science, Liverpool John Moores University, Liverpool, United KingdomLiverpool Centre for Cardiovascular Science at University of Liverpool, Liverpool John Moores University and Liverpool Heart & Chest Hospital, Liverpool, United KingdomResearch Institute for Sport and Exercise Science, Liverpool John Moores University, Liverpool, United KingdomLiverpool Centre for Cardiovascular Science at University of Liverpool, Liverpool John Moores University and Liverpool Heart & Chest Hospital, Liverpool, United KingdomIntroductionThe major pathophysiological symptom of the menopause affecting daily life is hot flushes, which are also associated with elevated cardiovascular disease risk. A hot flush is a sudden and intense heat sensation causing skin flushing and profuse sweating. Menopause-induced oestrogen deficiency could increase the sensitivity of skin blood vessels and sweat glands in postmenopausal women, which could result in more frequent and larger increases in skin blood flow in postmenopausal women consistent with hot flushes. Furthermore, oestrogen withdrawal could also alter the structure of the skin blood vessels and/or sweat glands which may also contribute to hot flushes. This trial aims to examine the function and structure of skin blood vessels and sweat glands in premenopausal and postmenopausal women.Methods and analysisThis is a single-centre multi-cohort observational study. Participants will attend the laboratory at Liverpool John Moores University (LJMU) on two separate occasions, ∼7 days apart. Visit 1 will consist of anthropometry, a blood sample and assessment of post-ganglionic skin blood vessel and sweat gland responsiveness via cutaneous microdialysis. At visit 2, participants will return for a skin punch biopsy. A between groups statistical analysis of the pre- and postmenopausal cohorts will be conducted in a blinded manner.Ethics and disseminationThe trial was approved by the North West - Greater Manchester South Research Ethics Committee (22/NW/0300) in the UK. The study adheres to The Declaration of Helsinki and is being conducted in accordance with the UK Policy Framework for Health and Social Care Research.DiscussionIdentifying functional and/or structural changes in skin blood vessels or sweat glands in women with hot flushes would increase our understanding of their cause(s) and side effects, and help to design effective treatments, including interventions that can manipulate the activity of the skin blood vessels and/or sweat glands via pharmacological or non-pharmacological methods. Trial registration numbersNCT06222073.https://www.frontiersin.org/articles/10.3389/fgwh.2025.1514960/fullskinmenopausehot flushblood flowsweating
spellingShingle Kirsty A. Roberts
Kirsty A. Roberts
Abigail Doyle
Helen Jones
Helen Jones
David A. Low
David A. Low
Getting under the skin of the menopausal hot flush: a protocol to examine skin function and structure in symptomatic postmenopausal women
Frontiers in Global Women's Health
skin
menopause
hot flush
blood flow
sweating
title Getting under the skin of the menopausal hot flush: a protocol to examine skin function and structure in symptomatic postmenopausal women
title_full Getting under the skin of the menopausal hot flush: a protocol to examine skin function and structure in symptomatic postmenopausal women
title_fullStr Getting under the skin of the menopausal hot flush: a protocol to examine skin function and structure in symptomatic postmenopausal women
title_full_unstemmed Getting under the skin of the menopausal hot flush: a protocol to examine skin function and structure in symptomatic postmenopausal women
title_short Getting under the skin of the menopausal hot flush: a protocol to examine skin function and structure in symptomatic postmenopausal women
title_sort getting under the skin of the menopausal hot flush a protocol to examine skin function and structure in symptomatic postmenopausal women
topic skin
menopause
hot flush
blood flow
sweating
url https://www.frontiersin.org/articles/10.3389/fgwh.2025.1514960/full
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