Exploring the functional diversity and metabolic activities of the human gut microbiome in Thai adults in response to a prebiotic diet
ABSTRACT Exploring dietary methods to alter microbial communities and metabolic functions is becoming an increasingly fascinating strategy for improving health. Copra meal hydrolysate (CMH) is alternatively used as a gut health supplement. However, the functional diversity and metabolic activities i...
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| Language: | English |
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American Society for Microbiology
2025-02-01
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| Series: | Microbiology Spectrum |
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| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.01599-24 |
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| author | Amornthep Kingkaw Preecha Patumcharoenpol Narissara Suratannon Massalin Nakphaichit Sittiruk Roytrakul Wanwipa Vongsangnak |
| author_facet | Amornthep Kingkaw Preecha Patumcharoenpol Narissara Suratannon Massalin Nakphaichit Sittiruk Roytrakul Wanwipa Vongsangnak |
| author_sort | Amornthep Kingkaw |
| collection | DOAJ |
| description | ABSTRACT Exploring dietary methods to alter microbial communities and metabolic functions is becoming an increasingly fascinating strategy for improving health. Copra meal hydrolysate (CMH) is alternatively used as a gut health supplement. However, the functional diversity and metabolic activities in gut microbiome in relation to CMH treatment remain largely unknown. Therefore, this study aimed to identify key predominant groups of bacterial species toward diversified metabolic functions, activities, and routes using metaproteomics. As a result, the integrative analysis of metaproteomic data revealed that seven key families across 11 dominant gut bacterial species were concerted. Consistently, across 76,206 proteins assigned to the metabolism of the 255,964 annotated proteins, short-chain fatty acid (SCFA) biosynthesis, lipopolysaccharide (LPS) biosynthesis, and bile acid (BA) metabolism were positively associated with CMH. Further identification of cooperative metabolic routes promisingly highlighted the importance of glycolysis/gluconeogenesis, tricarboxylic acid (TCA) cycle, inositol phosphate metabolism, steroid hormone biosynthesis, O-antigen repeat unit biosynthesis, and chloroalkane and chloroalkene degradation. This work presents an initial study of metaproteomics associated with prebiotic diet in a Thai population-based cohort in a developing Southeast Asian country.IMPORTANCEStudies primarily focused on the impact of CMH on gastrointestinal symptoms and gut microbial compositions. However, as the field moves toward understanding the relationship between microbiome and diet in relation to gut health, it is critical to evaluate how changes in metabolic activities relate to cooperative metabolic routes in the gut microbiome for promoting human health. Through the use of metaproteomics, our findings highlighted the key predominant groups of bacterial species, potential proteins, and their metabolic routes involved in gut metabolism. This study provides comprehensive insights into the fundamental relationship between microbiome and dietary supplements and suggests that metaproteomics is a powerful method for monitoring metabolic functions, activities, and routes in the gut microbiome. |
| format | Article |
| id | doaj-art-b7fc391c948c46b696f00883303d7771 |
| institution | Kabale University |
| issn | 2165-0497 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj-art-b7fc391c948c46b696f00883303d77712025-02-04T14:03:40ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972025-02-0113210.1128/spectrum.01599-24Exploring the functional diversity and metabolic activities of the human gut microbiome in Thai adults in response to a prebiotic dietAmornthep Kingkaw0Preecha Patumcharoenpol1Narissara Suratannon2Massalin Nakphaichit3Sittiruk Roytrakul4Wanwipa Vongsangnak5Interdisciplinary Graduate Program in Bioscience, Faculty of Science, Kasetsart University, Bangkok, ThailandInterdisciplinary Graduate Program in Bioscience, Faculty of Science, Kasetsart University, Bangkok, ThailandPediatric Allergy & Clinical Immunology Research Unit, Division of Allergy and Immunology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, the Thai Red Cross Society, Bangkok, ThailandDepartment of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok, ThailandFunctional Proteomics Technology Laboratory, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, ThailandDepartment of Zoology, Faculty of Sciences, Kasetsart University, Bangkok, ThailandABSTRACT Exploring dietary methods to alter microbial communities and metabolic functions is becoming an increasingly fascinating strategy for improving health. Copra meal hydrolysate (CMH) is alternatively used as a gut health supplement. However, the functional diversity and metabolic activities in gut microbiome in relation to CMH treatment remain largely unknown. Therefore, this study aimed to identify key predominant groups of bacterial species toward diversified metabolic functions, activities, and routes using metaproteomics. As a result, the integrative analysis of metaproteomic data revealed that seven key families across 11 dominant gut bacterial species were concerted. Consistently, across 76,206 proteins assigned to the metabolism of the 255,964 annotated proteins, short-chain fatty acid (SCFA) biosynthesis, lipopolysaccharide (LPS) biosynthesis, and bile acid (BA) metabolism were positively associated with CMH. Further identification of cooperative metabolic routes promisingly highlighted the importance of glycolysis/gluconeogenesis, tricarboxylic acid (TCA) cycle, inositol phosphate metabolism, steroid hormone biosynthesis, O-antigen repeat unit biosynthesis, and chloroalkane and chloroalkene degradation. This work presents an initial study of metaproteomics associated with prebiotic diet in a Thai population-based cohort in a developing Southeast Asian country.IMPORTANCEStudies primarily focused on the impact of CMH on gastrointestinal symptoms and gut microbial compositions. However, as the field moves toward understanding the relationship between microbiome and diet in relation to gut health, it is critical to evaluate how changes in metabolic activities relate to cooperative metabolic routes in the gut microbiome for promoting human health. Through the use of metaproteomics, our findings highlighted the key predominant groups of bacterial species, potential proteins, and their metabolic routes involved in gut metabolism. This study provides comprehensive insights into the fundamental relationship between microbiome and dietary supplements and suggests that metaproteomics is a powerful method for monitoring metabolic functions, activities, and routes in the gut microbiome.https://journals.asm.org/doi/10.1128/spectrum.01599-24copra meal hydrolysatehuman gut microbiomemetaproteomicsgut metabolismfunctional and metabolic activity |
| spellingShingle | Amornthep Kingkaw Preecha Patumcharoenpol Narissara Suratannon Massalin Nakphaichit Sittiruk Roytrakul Wanwipa Vongsangnak Exploring the functional diversity and metabolic activities of the human gut microbiome in Thai adults in response to a prebiotic diet Microbiology Spectrum copra meal hydrolysate human gut microbiome metaproteomics gut metabolism functional and metabolic activity |
| title | Exploring the functional diversity and metabolic activities of the human gut microbiome in Thai adults in response to a prebiotic diet |
| title_full | Exploring the functional diversity and metabolic activities of the human gut microbiome in Thai adults in response to a prebiotic diet |
| title_fullStr | Exploring the functional diversity and metabolic activities of the human gut microbiome in Thai adults in response to a prebiotic diet |
| title_full_unstemmed | Exploring the functional diversity and metabolic activities of the human gut microbiome in Thai adults in response to a prebiotic diet |
| title_short | Exploring the functional diversity and metabolic activities of the human gut microbiome in Thai adults in response to a prebiotic diet |
| title_sort | exploring the functional diversity and metabolic activities of the human gut microbiome in thai adults in response to a prebiotic diet |
| topic | copra meal hydrolysate human gut microbiome metaproteomics gut metabolism functional and metabolic activity |
| url | https://journals.asm.org/doi/10.1128/spectrum.01599-24 |
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