Characterizing the Genetic Basis of Winter Wheat Rust Resistance in Southern Kazakhstan

In an effort to enhance wheat’s resilience against rust diseases, our research explores the genetic underpinnings of resistance in a diverse collection of winter bread wheat accessions. Leaf rust (<i>Puccinia triticina</i>), yellow rust (<i>Puccinia striiformis</i> f. sp. <...

Full description

Saved in:
Bibliographic Details
Main Authors: Shynbolat Rsaliyev, Elena Gultyaeva, Olga Baranova, Alma Kokhmetova, Rahim Urazaliev, Ekaterina Shaydayuk, Akbope Abdikadyrova, Galiya Abugali
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Plants
Subjects:
Online Access:https://www.mdpi.com/2223-7747/14/7/1146
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850188274263392256
author Shynbolat Rsaliyev
Elena Gultyaeva
Olga Baranova
Alma Kokhmetova
Rahim Urazaliev
Ekaterina Shaydayuk
Akbope Abdikadyrova
Galiya Abugali
author_facet Shynbolat Rsaliyev
Elena Gultyaeva
Olga Baranova
Alma Kokhmetova
Rahim Urazaliev
Ekaterina Shaydayuk
Akbope Abdikadyrova
Galiya Abugali
author_sort Shynbolat Rsaliyev
collection DOAJ
description In an effort to enhance wheat’s resilience against rust diseases, our research explores the genetic underpinnings of resistance in a diverse collection of winter bread wheat accessions. Leaf rust (<i>Puccinia triticina</i>), yellow rust (<i>Puccinia striiformis</i> f. sp. <i>tritici</i>), and stem rust (<i>Puccinia graminis</i> f. sp. <i>tritici</i>) are significant threats to global wheat production. By leveraging host genetic resistance, we can improve disease management strategies. Our study evaluated 55 wheat accessions, including germplasm from Kazakhstan, from Uzbekistan, from Russia, from Kyrgyzstan, France, and CIMMYT under field conditions in southern Kazakhstan from 2022 to 2024. The results showed a robust resistance profile: 49.1% of accessions exhibited high to moderate resistance to leaf rust, 12.7% to yellow rust, and 30.9% to stem rust. Notably, ten accessions demonstrated resistance to multiple rust species, while seven showed resistance to two rusts. Twenty accessions were selected for further seedling resistance and molecular analysis. Three accessions proved resistant to six isolates of <i>P. triticina</i>, two to four isolates of <i>P. striiformis</i>, and four to five isolates of <i>P. graminis</i>. Although no genotypes were found to be universally resistant to all rust species at the seedling stage, two accessions—Bezostaya 100 (Russia) and KIZ 90 (Kazakhstan)—displayed consistent resistance to leaf and stem rust in both seedling and field evaluations. Molecular analysis revealed the presence of key resistance genes, including <i>Lr1</i>, <i>Lr3</i>, <i>Lr26</i>, <i>Lr34</i>, <i>Yr9</i>, <i>Yr18</i>, <i>Sr31</i>, <i>Sr57</i>, and the 1AL.1RS translocation. This work provides valuable insights into the genetic landscape of wheat rust resistance and contributes to the development of new wheat cultivars that can withstand these diseases, enhancing global food security.
format Article
id doaj-art-b7fa1d5812a14830881e409efa46dac5
institution OA Journals
issn 2223-7747
language English
publishDate 2025-04-01
publisher MDPI AG
record_format Article
series Plants
spelling doaj-art-b7fa1d5812a14830881e409efa46dac52025-08-20T02:15:54ZengMDPI AGPlants2223-77472025-04-01147114610.3390/plants14071146Characterizing the Genetic Basis of Winter Wheat Rust Resistance in Southern KazakhstanShynbolat Rsaliyev0Elena Gultyaeva1Olga Baranova2Alma Kokhmetova3Rahim Urazaliev4Ekaterina Shaydayuk5Akbope Abdikadyrova6Galiya Abugali7Kazakh Research Institute of Agriculture and Plant Growing, Almalybak 040909, KazakhstanAll Russian Institute of Plant Protection, Shosse Podbelskogo 3, 196608 St. Petersburg, RussiaAll Russian Institute of Plant Protection, Shosse Podbelskogo 3, 196608 St. Petersburg, RussiaInstitute of Plant Biology and Biotechnology, Almaty 050040, KazakhstanKazakh Research Institute of Agriculture and Plant Growing, Almalybak 040909, KazakhstanAll Russian Institute of Plant Protection, Shosse Podbelskogo 3, 196608 St. Petersburg, RussiaKazakh Research Institute of Agriculture and Plant Growing, Almalybak 040909, KazakhstanKazakh Research Institute of Agriculture and Plant Growing, Almalybak 040909, KazakhstanIn an effort to enhance wheat’s resilience against rust diseases, our research explores the genetic underpinnings of resistance in a diverse collection of winter bread wheat accessions. Leaf rust (<i>Puccinia triticina</i>), yellow rust (<i>Puccinia striiformis</i> f. sp. <i>tritici</i>), and stem rust (<i>Puccinia graminis</i> f. sp. <i>tritici</i>) are significant threats to global wheat production. By leveraging host genetic resistance, we can improve disease management strategies. Our study evaluated 55 wheat accessions, including germplasm from Kazakhstan, from Uzbekistan, from Russia, from Kyrgyzstan, France, and CIMMYT under field conditions in southern Kazakhstan from 2022 to 2024. The results showed a robust resistance profile: 49.1% of accessions exhibited high to moderate resistance to leaf rust, 12.7% to yellow rust, and 30.9% to stem rust. Notably, ten accessions demonstrated resistance to multiple rust species, while seven showed resistance to two rusts. Twenty accessions were selected for further seedling resistance and molecular analysis. Three accessions proved resistant to six isolates of <i>P. triticina</i>, two to four isolates of <i>P. striiformis</i>, and four to five isolates of <i>P. graminis</i>. Although no genotypes were found to be universally resistant to all rust species at the seedling stage, two accessions—Bezostaya 100 (Russia) and KIZ 90 (Kazakhstan)—displayed consistent resistance to leaf and stem rust in both seedling and field evaluations. Molecular analysis revealed the presence of key resistance genes, including <i>Lr1</i>, <i>Lr3</i>, <i>Lr26</i>, <i>Lr34</i>, <i>Yr9</i>, <i>Yr18</i>, <i>Sr31</i>, <i>Sr57</i>, and the 1AL.1RS translocation. This work provides valuable insights into the genetic landscape of wheat rust resistance and contributes to the development of new wheat cultivars that can withstand these diseases, enhancing global food security.https://www.mdpi.com/2223-7747/14/7/1146leaf rustyellow ruststem rustmolecular markersresistance geneswheat
spellingShingle Shynbolat Rsaliyev
Elena Gultyaeva
Olga Baranova
Alma Kokhmetova
Rahim Urazaliev
Ekaterina Shaydayuk
Akbope Abdikadyrova
Galiya Abugali
Characterizing the Genetic Basis of Winter Wheat Rust Resistance in Southern Kazakhstan
Plants
leaf rust
yellow rust
stem rust
molecular markers
resistance genes
wheat
title Characterizing the Genetic Basis of Winter Wheat Rust Resistance in Southern Kazakhstan
title_full Characterizing the Genetic Basis of Winter Wheat Rust Resistance in Southern Kazakhstan
title_fullStr Characterizing the Genetic Basis of Winter Wheat Rust Resistance in Southern Kazakhstan
title_full_unstemmed Characterizing the Genetic Basis of Winter Wheat Rust Resistance in Southern Kazakhstan
title_short Characterizing the Genetic Basis of Winter Wheat Rust Resistance in Southern Kazakhstan
title_sort characterizing the genetic basis of winter wheat rust resistance in southern kazakhstan
topic leaf rust
yellow rust
stem rust
molecular markers
resistance genes
wheat
url https://www.mdpi.com/2223-7747/14/7/1146
work_keys_str_mv AT shynbolatrsaliyev characterizingthegeneticbasisofwinterwheatrustresistanceinsouthernkazakhstan
AT elenagultyaeva characterizingthegeneticbasisofwinterwheatrustresistanceinsouthernkazakhstan
AT olgabaranova characterizingthegeneticbasisofwinterwheatrustresistanceinsouthernkazakhstan
AT almakokhmetova characterizingthegeneticbasisofwinterwheatrustresistanceinsouthernkazakhstan
AT rahimurazaliev characterizingthegeneticbasisofwinterwheatrustresistanceinsouthernkazakhstan
AT ekaterinashaydayuk characterizingthegeneticbasisofwinterwheatrustresistanceinsouthernkazakhstan
AT akbopeabdikadyrova characterizingthegeneticbasisofwinterwheatrustresistanceinsouthernkazakhstan
AT galiyaabugali characterizingthegeneticbasisofwinterwheatrustresistanceinsouthernkazakhstan