Heritable multiplex genetic engineering in rats using CRISPR/Cas9.
The CRISPR/Cas9 system has been proven to be an efficient gene-editing tool for genome modification of cells and organisms. Multiplex genetic engineering in rat holds a bright future for the study of complex disease. Here, we show that this system enables the simultaneous disruption of four genes (A...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0089413&type=printable |
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| _version_ | 1850024148699447296 |
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| author | Yuanwu Ma Bin Shen Xu Zhang Yingdong Lu Wei Chen Jing Ma Xingxu Huang Lianfeng Zhang |
| author_facet | Yuanwu Ma Bin Shen Xu Zhang Yingdong Lu Wei Chen Jing Ma Xingxu Huang Lianfeng Zhang |
| author_sort | Yuanwu Ma |
| collection | DOAJ |
| description | The CRISPR/Cas9 system has been proven to be an efficient gene-editing tool for genome modification of cells and organisms. Multiplex genetic engineering in rat holds a bright future for the study of complex disease. Here, we show that this system enables the simultaneous disruption of four genes (ApoE, B2m, Prf1, and Prkdc) in rats in one-step, by co-injection of Cas9 mRNA and sgRNAs into fertilized eggs. We further observed the gene modifications are germline transmittable, and confirmed the off-target mutagenesis and mosaicism are rarely detected by comprehensive analysis. Thus, the CRISPR/Cas9 system makes it possible to efficiently and reliably generate gene knock-out rats. |
| format | Article |
| id | doaj-art-b7f71f5423544ae3be6d2f98cd7aa455 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-b7f71f5423544ae3be6d2f98cd7aa4552025-08-20T03:01:11ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0193e8941310.1371/journal.pone.0089413Heritable multiplex genetic engineering in rats using CRISPR/Cas9.Yuanwu MaBin ShenXu ZhangYingdong LuWei ChenJing MaXingxu HuangLianfeng ZhangThe CRISPR/Cas9 system has been proven to be an efficient gene-editing tool for genome modification of cells and organisms. Multiplex genetic engineering in rat holds a bright future for the study of complex disease. Here, we show that this system enables the simultaneous disruption of four genes (ApoE, B2m, Prf1, and Prkdc) in rats in one-step, by co-injection of Cas9 mRNA and sgRNAs into fertilized eggs. We further observed the gene modifications are germline transmittable, and confirmed the off-target mutagenesis and mosaicism are rarely detected by comprehensive analysis. Thus, the CRISPR/Cas9 system makes it possible to efficiently and reliably generate gene knock-out rats.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0089413&type=printable |
| spellingShingle | Yuanwu Ma Bin Shen Xu Zhang Yingdong Lu Wei Chen Jing Ma Xingxu Huang Lianfeng Zhang Heritable multiplex genetic engineering in rats using CRISPR/Cas9. PLoS ONE |
| title | Heritable multiplex genetic engineering in rats using CRISPR/Cas9. |
| title_full | Heritable multiplex genetic engineering in rats using CRISPR/Cas9. |
| title_fullStr | Heritable multiplex genetic engineering in rats using CRISPR/Cas9. |
| title_full_unstemmed | Heritable multiplex genetic engineering in rats using CRISPR/Cas9. |
| title_short | Heritable multiplex genetic engineering in rats using CRISPR/Cas9. |
| title_sort | heritable multiplex genetic engineering in rats using crispr cas9 |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0089413&type=printable |
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