Aflatoxin B1 detoxification potential of viable and killed Lactobacillus rhamnosus by in vitro method
Aflatoxin B1 (AFB1) is a potent mycotoxin produced by Aspergillus flavus and related fungi, posing severe health risks to humans and animals. This study evaluated the aflatoxin detoxification ability of viable and killed Lactobacillus rhamnosus using an in vitro binding assay. Five experimental grou...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Director of Academics and Research, Kerala Veterinary and Animal Sciences University
2025-06-01
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| Series: | Journal of Veterinary and Animal Sciences |
| Online Access: | https://www.jvas.in/article/aflatoxin-b1-detoxification-potential-of-viable-and-killed-lactobacillus-rhamnosus-by-in-vitro-method |
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| Summary: | Aflatoxin B1 (AFB1) is a potent mycotoxin produced by Aspergillus flavus and related fungi, posing severe health risks to humans and animals. This study evaluated the aflatoxin detoxification ability of viable and killed Lactobacillus rhamnosus using an in vitro binding assay. Five experimental groups were included: Treatment 1 contained viable L. rhamnosus with poultry layer feed adjusted to 30 ppb AFB1 by adding contaminated maize, while treatment 2 used killed L. rhamnosus under the same conditions. A positive control was maintained with 30 ppb AFB1 alone. Two negative controls were prepared using feed with naturally occurring AFB1 (concentration determined after preparation) and supplemented with either viable or killed L. rhamnosus. AFB1 levels were quantified at 3h and 6h post-treatment using ultra-highperformance liquid chromatography (UHPLC) following immunoaffinity column (IAC) cleanup. Statistical analysis was performed using RMANOVA and one-sample t-tests. The results demonstrated a significant reduction in AFB1 levels in the presence of both viable and killed L. rhamnosus. AFB1 reduction ranged from 24.4 to 28.2 in Treatment 1 and Treatment 2, while in the negative controls, the reduction was between 16.7 and 17.8. These findings highlight the potential application of L. rhamnosus in aflatoxin detoxification strategies, particularly for improving feed safety in the poultry industry. |
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| ISSN: | 0971-0701 2582-0605 |