RETRACTED ARTICLE: Pre-activation of mesenchymal stem cells with TNF-α, IL-1β and nitric oxide enhances its paracrine effects on radiation-induced intestinal injury
Abstract Conditioned medium from mesenchymal stem cells (MSC-CM) may represent a promising alternative to MSCs transplantation, however, the low concentrations of growth factors in non-activated MSC-CM hamper its clinical application. Recent data indicated that the paracrine potential of MSCs could...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2015-03-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/srep08718 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850185782004809728 |
|---|---|
| author | Hao Chen Xiao-Hui Min Qi-Yi Wang Felix W. Leung Liu Shi Yu Zhou Tao Yu Chuan-Ming Wang Geng An Wei-Hong Sha Qi-Kui Chen |
| author_facet | Hao Chen Xiao-Hui Min Qi-Yi Wang Felix W. Leung Liu Shi Yu Zhou Tao Yu Chuan-Ming Wang Geng An Wei-Hong Sha Qi-Kui Chen |
| author_sort | Hao Chen |
| collection | DOAJ |
| description | Abstract Conditioned medium from mesenchymal stem cells (MSC-CM) may represent a promising alternative to MSCs transplantation, however, the low concentrations of growth factors in non-activated MSC-CM hamper its clinical application. Recent data indicated that the paracrine potential of MSCs could be enhanced by inflammatory factors. Herein, we pre-activated bone-marrow-derived MSCs under radiation-induced inflammatory condition (MSCIEC-6(IR)) and investigated the evidence and mechanism for the differential effects of MSC-CMIEC-6(IR) and non-activated MSC-CM on radiation-induced intestinal injury (RIII). Systemic infusion of MSC-CMIEC-6(IR), but not non-activated MSC-CM, dramatically improved intestinal damage and survival of irradiated rats. Such benefits may involve the modulation of epithelial regeneration and inflammation, as indicated by the regeneration of intestinal epithelial/stem cells, the regulation of the pro-/anti-inflammatory cytokine balance. The mechanism for the superior paracrine efficacy of MSCIEC-6(IR) is related to a higher secretion of regenerative, immunomodulatory and trafficking molecules, including the pivotal factor IGF-1, induced by TNF-α, IL-1β and nitric oxide partially via a heme oxygenase-1 dependent mechanism. Together, our findings suggest that pre-activation of MSCs with TNF-α, IL-1β and nitric oxide enhances its paracine effects on RIII via a heme oxygenase-1 dependent mechanism, which may help us to maximize the paracrine potential of MSCs. |
| format | Article |
| id | doaj-art-b7e67251629944de8a362a4184ec17a7 |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2015-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-b7e67251629944de8a362a4184ec17a72025-08-20T02:16:34ZengNature PortfolioScientific Reports2045-23222015-03-015111410.1038/srep08718RETRACTED ARTICLE: Pre-activation of mesenchymal stem cells with TNF-α, IL-1β and nitric oxide enhances its paracrine effects on radiation-induced intestinal injuryHao Chen0Xiao-Hui Min1Qi-Yi Wang2Felix W. Leung3Liu Shi4Yu Zhou5Tao Yu6Chuan-Ming Wang7Geng An8Wei-Hong Sha9Qi-Kui Chen10Department of Gastroenterology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen UniversityDepartment of Gastroenterology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen UniversityDepartment of Gastroenterology, Guangdong General Hospital and Guangdong Academy of Medical SciencesDivision of Gastroenterology, David Geffen School of Medicine at University of CaliforniaDepartment of Gastroenterology, Ganzhou People's HospitalDepartment of Gastroenterology, Affiliated Hospital of Guangdong Medical CollegeDepartment of Gastroenterology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen UniversityDepartment of Neurology, Shenzhen Sixth People's HospitalDepartment of Reproductive Medicine Center, The Third Affiliated Hospital of Guangzhou Medical UniversityDepartment of Gastroenterology, Guangdong General Hospital and Guangdong Academy of Medical SciencesDepartment of Gastroenterology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen UniversityAbstract Conditioned medium from mesenchymal stem cells (MSC-CM) may represent a promising alternative to MSCs transplantation, however, the low concentrations of growth factors in non-activated MSC-CM hamper its clinical application. Recent data indicated that the paracrine potential of MSCs could be enhanced by inflammatory factors. Herein, we pre-activated bone-marrow-derived MSCs under radiation-induced inflammatory condition (MSCIEC-6(IR)) and investigated the evidence and mechanism for the differential effects of MSC-CMIEC-6(IR) and non-activated MSC-CM on radiation-induced intestinal injury (RIII). Systemic infusion of MSC-CMIEC-6(IR), but not non-activated MSC-CM, dramatically improved intestinal damage and survival of irradiated rats. Such benefits may involve the modulation of epithelial regeneration and inflammation, as indicated by the regeneration of intestinal epithelial/stem cells, the regulation of the pro-/anti-inflammatory cytokine balance. The mechanism for the superior paracrine efficacy of MSCIEC-6(IR) is related to a higher secretion of regenerative, immunomodulatory and trafficking molecules, including the pivotal factor IGF-1, induced by TNF-α, IL-1β and nitric oxide partially via a heme oxygenase-1 dependent mechanism. Together, our findings suggest that pre-activation of MSCs with TNF-α, IL-1β and nitric oxide enhances its paracine effects on RIII via a heme oxygenase-1 dependent mechanism, which may help us to maximize the paracrine potential of MSCs.https://doi.org/10.1038/srep08718 |
| spellingShingle | Hao Chen Xiao-Hui Min Qi-Yi Wang Felix W. Leung Liu Shi Yu Zhou Tao Yu Chuan-Ming Wang Geng An Wei-Hong Sha Qi-Kui Chen RETRACTED ARTICLE: Pre-activation of mesenchymal stem cells with TNF-α, IL-1β and nitric oxide enhances its paracrine effects on radiation-induced intestinal injury Scientific Reports |
| title | RETRACTED ARTICLE: Pre-activation of mesenchymal stem cells with TNF-α, IL-1β and nitric oxide enhances its paracrine effects on radiation-induced intestinal injury |
| title_full | RETRACTED ARTICLE: Pre-activation of mesenchymal stem cells with TNF-α, IL-1β and nitric oxide enhances its paracrine effects on radiation-induced intestinal injury |
| title_fullStr | RETRACTED ARTICLE: Pre-activation of mesenchymal stem cells with TNF-α, IL-1β and nitric oxide enhances its paracrine effects on radiation-induced intestinal injury |
| title_full_unstemmed | RETRACTED ARTICLE: Pre-activation of mesenchymal stem cells with TNF-α, IL-1β and nitric oxide enhances its paracrine effects on radiation-induced intestinal injury |
| title_short | RETRACTED ARTICLE: Pre-activation of mesenchymal stem cells with TNF-α, IL-1β and nitric oxide enhances its paracrine effects on radiation-induced intestinal injury |
| title_sort | retracted article pre activation of mesenchymal stem cells with tnf α il 1β and nitric oxide enhances its paracrine effects on radiation induced intestinal injury |
| url | https://doi.org/10.1038/srep08718 |
| work_keys_str_mv | AT haochen retractedarticlepreactivationofmesenchymalstemcellswithtnfail1bandnitricoxideenhancesitsparacrineeffectsonradiationinducedintestinalinjury AT xiaohuimin retractedarticlepreactivationofmesenchymalstemcellswithtnfail1bandnitricoxideenhancesitsparacrineeffectsonradiationinducedintestinalinjury AT qiyiwang retractedarticlepreactivationofmesenchymalstemcellswithtnfail1bandnitricoxideenhancesitsparacrineeffectsonradiationinducedintestinalinjury AT felixwleung retractedarticlepreactivationofmesenchymalstemcellswithtnfail1bandnitricoxideenhancesitsparacrineeffectsonradiationinducedintestinalinjury AT liushi retractedarticlepreactivationofmesenchymalstemcellswithtnfail1bandnitricoxideenhancesitsparacrineeffectsonradiationinducedintestinalinjury AT yuzhou retractedarticlepreactivationofmesenchymalstemcellswithtnfail1bandnitricoxideenhancesitsparacrineeffectsonradiationinducedintestinalinjury AT taoyu retractedarticlepreactivationofmesenchymalstemcellswithtnfail1bandnitricoxideenhancesitsparacrineeffectsonradiationinducedintestinalinjury AT chuanmingwang retractedarticlepreactivationofmesenchymalstemcellswithtnfail1bandnitricoxideenhancesitsparacrineeffectsonradiationinducedintestinalinjury AT gengan retractedarticlepreactivationofmesenchymalstemcellswithtnfail1bandnitricoxideenhancesitsparacrineeffectsonradiationinducedintestinalinjury AT weihongsha retractedarticlepreactivationofmesenchymalstemcellswithtnfail1bandnitricoxideenhancesitsparacrineeffectsonradiationinducedintestinalinjury AT qikuichen retractedarticlepreactivationofmesenchymalstemcellswithtnfail1bandnitricoxideenhancesitsparacrineeffectsonradiationinducedintestinalinjury |