Advancing Soft Tissue Reconstruction with a Ready-to-Use Human Adipose Allograft
Soft tissue reconstruction remains a challenge in clinical practice, particularly for restoring substantial volume loss due to surgical resections or contour deformities. Current methods, such as autologous fat transplantation, have limitations, including donor site morbidity and insufficient tissue...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-06-01
|
| Series: | Bioengineering |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2306-5354/12/6/612 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849433654013460480 |
|---|---|
| author | Victor Fanniel Ihab Atawneh Jonathan Savoie Michelle Izaguirre-Ramirez Joanna Marquez Christopher Khorsandi Shauna Hill |
| author_facet | Victor Fanniel Ihab Atawneh Jonathan Savoie Michelle Izaguirre-Ramirez Joanna Marquez Christopher Khorsandi Shauna Hill |
| author_sort | Victor Fanniel |
| collection | DOAJ |
| description | Soft tissue reconstruction remains a challenge in clinical practice, particularly for restoring substantial volume loss due to surgical resections or contour deformities. Current methods, such as autologous fat transplantation, have limitations, including donor site morbidity and insufficient tissue availability, necessitating an innovative approach. This study characterizes alloClae, a minimally manipulated human-derived adipose allograft prepared using a detergent-based protocol to reduce DNA content while preserving adipose tissue structure. Proteomic analysis revealed that alloClae retains key native proteins critical for graft integration with the host and stability, with key extracellular matrix (ECM) components, collagens, elastins, and laminin, which are more concentrated as a result of the detergent-based protocol. Biocompatibility of alloClae was assessed in vitro using cytotoxicity and cell viability assays in fibroblast cultures, revealing no adverse effects on cell viability, membrane integrity, or oxidative stress. Additionally, in vitro studies with adipose-derived stem cells (ASCs) demonstrated attachment and differentiation, with lipid droplet accumulation observed by day 14, indicating support for adipogenesis. A 6-month longitudinal study in athymic mice showed stable graft retention, host cell infiltration, and formation of new adipocytes and vasculature within alloClae by 3 months. The findings highlight alloClae’s ability to support host-driven adipogenesis and angiogenesis while maintaining graft stability throughout the study period. It presents a promising alternative to the existing graft materials, offering a clinically translatable solution for soft tissue reconstruction. |
| format | Article |
| id | doaj-art-b7e28f30e898435e912d0dc5a8c48383 |
| institution | Kabale University |
| issn | 2306-5354 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj-art-b7e28f30e898435e912d0dc5a8c483832025-08-20T03:26:57ZengMDPI AGBioengineering2306-53542025-06-0112661210.3390/bioengineering12060612Advancing Soft Tissue Reconstruction with a Ready-to-Use Human Adipose AllograftVictor Fanniel0Ihab Atawneh1Jonathan Savoie2Michelle Izaguirre-Ramirez3Joanna Marquez4Christopher Khorsandi5Shauna Hill6Shauna Hill, RegenTX Labs, LLC, 3463 Magic Dr Ste 315, San Antonio, TX 78229, USAShauna Hill, RegenTX Labs, LLC, 3463 Magic Dr Ste 315, San Antonio, TX 78229, USAShauna Hill, RegenTX Labs, LLC, 3463 Magic Dr Ste 315, San Antonio, TX 78229, USAShauna Hill, RegenTX Labs, LLC, 3463 Magic Dr Ste 315, San Antonio, TX 78229, USAShauna Hill, RegenTX Labs, LLC, 3463 Magic Dr Ste 315, San Antonio, TX 78229, USAVIP Plastic Surgery, 2779 Sunridge Heights Pkwy Ste 100, Henderson, NV 89052, USAShauna Hill, RegenTX Labs, LLC, 3463 Magic Dr Ste 315, San Antonio, TX 78229, USASoft tissue reconstruction remains a challenge in clinical practice, particularly for restoring substantial volume loss due to surgical resections or contour deformities. Current methods, such as autologous fat transplantation, have limitations, including donor site morbidity and insufficient tissue availability, necessitating an innovative approach. This study characterizes alloClae, a minimally manipulated human-derived adipose allograft prepared using a detergent-based protocol to reduce DNA content while preserving adipose tissue structure. Proteomic analysis revealed that alloClae retains key native proteins critical for graft integration with the host and stability, with key extracellular matrix (ECM) components, collagens, elastins, and laminin, which are more concentrated as a result of the detergent-based protocol. Biocompatibility of alloClae was assessed in vitro using cytotoxicity and cell viability assays in fibroblast cultures, revealing no adverse effects on cell viability, membrane integrity, or oxidative stress. Additionally, in vitro studies with adipose-derived stem cells (ASCs) demonstrated attachment and differentiation, with lipid droplet accumulation observed by day 14, indicating support for adipogenesis. A 6-month longitudinal study in athymic mice showed stable graft retention, host cell infiltration, and formation of new adipocytes and vasculature within alloClae by 3 months. The findings highlight alloClae’s ability to support host-driven adipogenesis and angiogenesis while maintaining graft stability throughout the study period. It presents a promising alternative to the existing graft materials, offering a clinically translatable solution for soft tissue reconstruction.https://www.mdpi.com/2306-5354/12/6/612adipose allograftminimal manipulationbiocompatibilitysoft tissue reconstructionadipose extracellular matrixtissue engineering |
| spellingShingle | Victor Fanniel Ihab Atawneh Jonathan Savoie Michelle Izaguirre-Ramirez Joanna Marquez Christopher Khorsandi Shauna Hill Advancing Soft Tissue Reconstruction with a Ready-to-Use Human Adipose Allograft Bioengineering adipose allograft minimal manipulation biocompatibility soft tissue reconstruction adipose extracellular matrix tissue engineering |
| title | Advancing Soft Tissue Reconstruction with a Ready-to-Use Human Adipose Allograft |
| title_full | Advancing Soft Tissue Reconstruction with a Ready-to-Use Human Adipose Allograft |
| title_fullStr | Advancing Soft Tissue Reconstruction with a Ready-to-Use Human Adipose Allograft |
| title_full_unstemmed | Advancing Soft Tissue Reconstruction with a Ready-to-Use Human Adipose Allograft |
| title_short | Advancing Soft Tissue Reconstruction with a Ready-to-Use Human Adipose Allograft |
| title_sort | advancing soft tissue reconstruction with a ready to use human adipose allograft |
| topic | adipose allograft minimal manipulation biocompatibility soft tissue reconstruction adipose extracellular matrix tissue engineering |
| url | https://www.mdpi.com/2306-5354/12/6/612 |
| work_keys_str_mv | AT victorfanniel advancingsofttissuereconstructionwithareadytousehumanadiposeallograft AT ihabatawneh advancingsofttissuereconstructionwithareadytousehumanadiposeallograft AT jonathansavoie advancingsofttissuereconstructionwithareadytousehumanadiposeallograft AT michelleizaguirreramirez advancingsofttissuereconstructionwithareadytousehumanadiposeallograft AT joannamarquez advancingsofttissuereconstructionwithareadytousehumanadiposeallograft AT christopherkhorsandi advancingsofttissuereconstructionwithareadytousehumanadiposeallograft AT shaunahill advancingsofttissuereconstructionwithareadytousehumanadiposeallograft |