Simultaneous Determination of Paracetamol, Acetyl Salicylic Acid, Mefenamic Acid and Cetirizine Dihydrochloride in the Pharmaceutical Dosage Form
A simple, specific, accurate and economical isocratic reversed phase liquid chromatographic (RP-HPLC) method was developed and subsequently validated for the determination of paracetamol, acetyl salicylic acid, mefenamic acid and cetirizine dihydrochloride. Separation was achieved with a Nucleodur 1...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2010/241876 |
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| author | Freddy H. Havaldar Dharmendra L. Vairal |
| author_facet | Freddy H. Havaldar Dharmendra L. Vairal |
| author_sort | Freddy H. Havaldar |
| collection | DOAJ |
| description | A simple, specific, accurate and economical isocratic reversed phase liquid chromatographic (RP-HPLC) method was developed and subsequently validated for the determination of paracetamol, acetyl salicylic acid, mefenamic acid and cetirizine dihydrochloride. Separation was achieved with a Nucleodur 100 C–18 column having 250 × 4.6 mm i.d. with 5 µm particle size and disodium hydrogen phosphate buffer adjusted to pH 6.5 using diluted orthophosphoric acid and acetonitrile (60:40 v∕v) as eluent at a constant flow rate of 1.0 mL per min. UV detection was performed at 220 nm. The retention time of acetyl salicylic acid, paracetamol, mefenamic acid and cetirizine dihydrochloride were 2.01 min, 2.92 min, 4.91 min and 10.2 min respectively. This method is simple, rapid and selective and can be used for routine analysis of analgesic and antipyretic drugs in pharmaceutical formulations. The proposed method was validated and successfully used for estimation of paracetamol, acetyl salicylic acid, mefenamic acid and cetirizine dihydrochloride in the pharmaceutical dosage form. |
| format | Article |
| id | doaj-art-b7e0b7a952b44e3c8ccb13ab8f4b8ba4 |
| institution | Kabale University |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-b7e0b7a952b44e3c8ccb13ab8f4b8ba42025-02-03T01:30:38ZengWileyE-Journal of Chemistry0973-49452090-98102010-01-017S1S495S50310.1155/2010/241876Simultaneous Determination of Paracetamol, Acetyl Salicylic Acid, Mefenamic Acid and Cetirizine Dihydrochloride in the Pharmaceutical Dosage FormFreddy H. Havaldar0Dharmendra L. Vairal1Nadkarny - Sacasa Research Laboratory Department of Chemistry, St.Xavier's College, Mumbai-400001, IndiaNadkarny - Sacasa Research Laboratory Department of Chemistry, St.Xavier's College, Mumbai-400001, IndiaA simple, specific, accurate and economical isocratic reversed phase liquid chromatographic (RP-HPLC) method was developed and subsequently validated for the determination of paracetamol, acetyl salicylic acid, mefenamic acid and cetirizine dihydrochloride. Separation was achieved with a Nucleodur 100 C–18 column having 250 × 4.6 mm i.d. with 5 µm particle size and disodium hydrogen phosphate buffer adjusted to pH 6.5 using diluted orthophosphoric acid and acetonitrile (60:40 v∕v) as eluent at a constant flow rate of 1.0 mL per min. UV detection was performed at 220 nm. The retention time of acetyl salicylic acid, paracetamol, mefenamic acid and cetirizine dihydrochloride were 2.01 min, 2.92 min, 4.91 min and 10.2 min respectively. This method is simple, rapid and selective and can be used for routine analysis of analgesic and antipyretic drugs in pharmaceutical formulations. The proposed method was validated and successfully used for estimation of paracetamol, acetyl salicylic acid, mefenamic acid and cetirizine dihydrochloride in the pharmaceutical dosage form.http://dx.doi.org/10.1155/2010/241876 |
| spellingShingle | Freddy H. Havaldar Dharmendra L. Vairal Simultaneous Determination of Paracetamol, Acetyl Salicylic Acid, Mefenamic Acid and Cetirizine Dihydrochloride in the Pharmaceutical Dosage Form E-Journal of Chemistry |
| title | Simultaneous Determination of Paracetamol, Acetyl Salicylic Acid, Mefenamic Acid and Cetirizine Dihydrochloride in the Pharmaceutical Dosage Form |
| title_full | Simultaneous Determination of Paracetamol, Acetyl Salicylic Acid, Mefenamic Acid and Cetirizine Dihydrochloride in the Pharmaceutical Dosage Form |
| title_fullStr | Simultaneous Determination of Paracetamol, Acetyl Salicylic Acid, Mefenamic Acid and Cetirizine Dihydrochloride in the Pharmaceutical Dosage Form |
| title_full_unstemmed | Simultaneous Determination of Paracetamol, Acetyl Salicylic Acid, Mefenamic Acid and Cetirizine Dihydrochloride in the Pharmaceutical Dosage Form |
| title_short | Simultaneous Determination of Paracetamol, Acetyl Salicylic Acid, Mefenamic Acid and Cetirizine Dihydrochloride in the Pharmaceutical Dosage Form |
| title_sort | simultaneous determination of paracetamol acetyl salicylic acid mefenamic acid and cetirizine dihydrochloride in the pharmaceutical dosage form |
| url | http://dx.doi.org/10.1155/2010/241876 |
| work_keys_str_mv | AT freddyhhavaldar simultaneousdeterminationofparacetamolacetylsalicylicacidmefenamicacidandcetirizinedihydrochlorideinthepharmaceuticaldosageform AT dharmendralvairal simultaneousdeterminationofparacetamolacetylsalicylicacidmefenamicacidandcetirizinedihydrochlorideinthepharmaceuticaldosageform |