Elucidating the molecular basis of salt tolerance in potatoes through miRNA expression and phenotypic analysis
Abstract Potatoes are a critical staple crop worldwide, yet their yield is significantly constrained by salt stress. Understanding and enhancing salt tolerance in potatoes is crucial for ensuring food security. This study evaluated the salt tolerance of 17 diverse potato varieties using principal co...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-025-86276-5 |
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| author | Caicai Lin Shuangshuang Zheng Kui Liu Ru Yu Peiyan Guan Baigeng Hu Lingling Jiang Mengyu Su Guodong Hu Qingshuai Chen Xia Zhang |
| author_facet | Caicai Lin Shuangshuang Zheng Kui Liu Ru Yu Peiyan Guan Baigeng Hu Lingling Jiang Mengyu Su Guodong Hu Qingshuai Chen Xia Zhang |
| author_sort | Caicai Lin |
| collection | DOAJ |
| description | Abstract Potatoes are a critical staple crop worldwide, yet their yield is significantly constrained by salt stress. Understanding and enhancing salt tolerance in potatoes is crucial for ensuring food security. This study evaluated the salt tolerance of 17 diverse potato varieties using principal component analysis, membership function analysis, cluster analysis, and stepwise regression analysis. Comprehensive evaluation based on morphological, physiological, and biochemical indicators divided the varieties into three categories, identifying Z1264-1, Z700-1, Z943-1, Z1266-1, Z510-1, and Z1076-1 as having strong salt tolerance. Regression equations established stem thickness, root length, and catalase activity as rapid identification markers for salt tolerance in tetraploid potatoes. Transcriptome analysis of the highly tolerant variety Z1076-1 identified 68 differentially expressed miRNAs (DE miRNAs). qRT-PCR validation for eight randomly selected DE miRNAs confirmed consistent expression trends with transcriptome data. Predicted target genes of these miRNAs are involved in calcium channel signaling, osmotic regulation, plant hormone signaling, and reactive oxygen species clearance. Our findings provide valuable insights for the identification and screening of salt-tolerant potato germplasms. The findings also lay the foundation for studying molecular mechanisms of salt tolerance and advancing genetic breeding efforts to cultivate more resilient potato varieties. |
| format | Article |
| id | doaj-art-b7de5d3bdfd147dda3bd0a6153287fb4 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-b7de5d3bdfd147dda3bd0a6153287fb42025-01-26T12:28:30ZengNature PortfolioScientific Reports2045-23222025-01-0115111510.1038/s41598-025-86276-5Elucidating the molecular basis of salt tolerance in potatoes through miRNA expression and phenotypic analysisCaicai Lin0Shuangshuang Zheng1Kui Liu2Ru Yu3Peiyan Guan4Baigeng Hu5Lingling Jiang6Mengyu Su7Guodong Hu8Qingshuai Chen9Xia Zhang10Shandong Provincial Key Laboratory of Biophysics, Institute of Biophysics, Dezhou UniversityShandong Provincial Key Laboratory of Biophysics, Institute of Biophysics, Dezhou UniversityShandong Provincial Key Laboratory of Biophysics, Institute of Biophysics, Dezhou UniversityShandong Provincial Key Laboratory of Biophysics, Institute of Biophysics, Dezhou UniversityBiology Department, Dezhou UniversityNational Engineering Research Center for PotatoShandong Provincial Key Laboratory of Biophysics, Institute of Biophysics, Dezhou UniversityShandong Provincial Key Laboratory of Biophysics, Institute of Biophysics, Dezhou UniversityShandong Provincial Key Laboratory of Biophysics, Institute of Biophysics, Dezhou UniversityShandong Provincial Key Laboratory of Biophysics, Institute of Biophysics, Dezhou UniversityShandong Provincial Key Laboratory of Biophysics, Institute of Biophysics, Dezhou UniversityAbstract Potatoes are a critical staple crop worldwide, yet their yield is significantly constrained by salt stress. Understanding and enhancing salt tolerance in potatoes is crucial for ensuring food security. This study evaluated the salt tolerance of 17 diverse potato varieties using principal component analysis, membership function analysis, cluster analysis, and stepwise regression analysis. Comprehensive evaluation based on morphological, physiological, and biochemical indicators divided the varieties into three categories, identifying Z1264-1, Z700-1, Z943-1, Z1266-1, Z510-1, and Z1076-1 as having strong salt tolerance. Regression equations established stem thickness, root length, and catalase activity as rapid identification markers for salt tolerance in tetraploid potatoes. Transcriptome analysis of the highly tolerant variety Z1076-1 identified 68 differentially expressed miRNAs (DE miRNAs). qRT-PCR validation for eight randomly selected DE miRNAs confirmed consistent expression trends with transcriptome data. Predicted target genes of these miRNAs are involved in calcium channel signaling, osmotic regulation, plant hormone signaling, and reactive oxygen species clearance. Our findings provide valuable insights for the identification and screening of salt-tolerant potato germplasms. The findings also lay the foundation for studying molecular mechanisms of salt tolerance and advancing genetic breeding efforts to cultivate more resilient potato varieties.https://doi.org/10.1038/s41598-025-86276-5PotatoSalt toleranceComprehensive evaluationMiRNA-SeqDifferential expression |
| spellingShingle | Caicai Lin Shuangshuang Zheng Kui Liu Ru Yu Peiyan Guan Baigeng Hu Lingling Jiang Mengyu Su Guodong Hu Qingshuai Chen Xia Zhang Elucidating the molecular basis of salt tolerance in potatoes through miRNA expression and phenotypic analysis Scientific Reports Potato Salt tolerance Comprehensive evaluation MiRNA-Seq Differential expression |
| title | Elucidating the molecular basis of salt tolerance in potatoes through miRNA expression and phenotypic analysis |
| title_full | Elucidating the molecular basis of salt tolerance in potatoes through miRNA expression and phenotypic analysis |
| title_fullStr | Elucidating the molecular basis of salt tolerance in potatoes through miRNA expression and phenotypic analysis |
| title_full_unstemmed | Elucidating the molecular basis of salt tolerance in potatoes through miRNA expression and phenotypic analysis |
| title_short | Elucidating the molecular basis of salt tolerance in potatoes through miRNA expression and phenotypic analysis |
| title_sort | elucidating the molecular basis of salt tolerance in potatoes through mirna expression and phenotypic analysis |
| topic | Potato Salt tolerance Comprehensive evaluation MiRNA-Seq Differential expression |
| url | https://doi.org/10.1038/s41598-025-86276-5 |
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