Leptin Effects on the Regenerative Capacity of Human Periodontal Cells

Obesity is increasing throughout the globe and characterized by excess adipose tissue, which represents a complex endocrine organ. Adipose tissue secrets bioactive molecules called adipokines, which act at endocrine, paracrine, and autocrine levels. Obesity has recently been shown to be associated w...

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Main Authors: Marjan Nokhbehsaim, Sema Keser, Andressa Vilas Boas Nogueira, Andreas Jäger, Søren Jepsen, Joni Augusto Cirelli, Christoph Bourauel, Sigrun Eick, James Deschner
Format: Article
Language:English
Published: Wiley 2014-01-01
Series:International Journal of Endocrinology
Online Access:http://dx.doi.org/10.1155/2014/180304
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author Marjan Nokhbehsaim
Sema Keser
Andressa Vilas Boas Nogueira
Andreas Jäger
Søren Jepsen
Joni Augusto Cirelli
Christoph Bourauel
Sigrun Eick
James Deschner
author_facet Marjan Nokhbehsaim
Sema Keser
Andressa Vilas Boas Nogueira
Andreas Jäger
Søren Jepsen
Joni Augusto Cirelli
Christoph Bourauel
Sigrun Eick
James Deschner
author_sort Marjan Nokhbehsaim
collection DOAJ
description Obesity is increasing throughout the globe and characterized by excess adipose tissue, which represents a complex endocrine organ. Adipose tissue secrets bioactive molecules called adipokines, which act at endocrine, paracrine, and autocrine levels. Obesity has recently been shown to be associated with periodontitis, a disease characterized by the irreversible destruction of the tooth-supporting tissues, that is, periodontium, and also with compromised periodontal healing. Although the underlying mechanisms for these associations are not clear yet, increased levels of proinflammatory adipokines, such as leptin, as found in obese individuals, might be a critical pathomechanistic link. The objective of this study was to examine the impact of leptin on the regenerative capacity of human periodontal ligament (PDL) cells and also to study the local leptin production by these cells. Leptin caused a significant downregulation of growth (TGFβ1, and VEGFA) and transcription (RUNX2) factors as well as matrix molecules (collagen, and periostin) and inhibited SMAD signaling under regenerative conditions. Moreover, the local expression of leptin and its full-length receptor was significantly downregulated by inflammatory, microbial, and biomechanical signals. This study demonstrates that the hormone leptin negatively interferes with the regenerative capacity of PDL cells, suggesting leptin as a pathomechanistic link between obesity and compromised periodontal healing.
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spelling doaj-art-b7d9086351ff47289101b15d2d17c64b2025-08-20T02:19:18ZengWileyInternational Journal of Endocrinology1687-83371687-83452014-01-01201410.1155/2014/180304180304Leptin Effects on the Regenerative Capacity of Human Periodontal CellsMarjan Nokhbehsaim0Sema Keser1Andressa Vilas Boas Nogueira2Andreas Jäger3Søren Jepsen4Joni Augusto Cirelli5Christoph Bourauel6Sigrun Eick7James Deschner8Experimental Dento-Maxillo-Facial Medicine, University of Bonn, 53111 Bonn, GermanyClinical Research Unit 208, University of Bonn, 53111 Bonn, GermanyClinical Research Unit 208, University of Bonn, 53111 Bonn, GermanyClinical Research Unit 208, University of Bonn, 53111 Bonn, GermanyClinical Research Unit 208, University of Bonn, 53111 Bonn, GermanyDepartment of Diagnosis and Surgery, School of Dentistry, UNESP, 14801-903 Araraquara, SP, BrazilClinical Research Unit 208, University of Bonn, 53111 Bonn, GermanyDepartment of Periodontology, Laboratory of Oral Microbiology, University of Bern, 3010 Bern, SwitzerlandExperimental Dento-Maxillo-Facial Medicine, University of Bonn, 53111 Bonn, GermanyObesity is increasing throughout the globe and characterized by excess adipose tissue, which represents a complex endocrine organ. Adipose tissue secrets bioactive molecules called adipokines, which act at endocrine, paracrine, and autocrine levels. Obesity has recently been shown to be associated with periodontitis, a disease characterized by the irreversible destruction of the tooth-supporting tissues, that is, periodontium, and also with compromised periodontal healing. Although the underlying mechanisms for these associations are not clear yet, increased levels of proinflammatory adipokines, such as leptin, as found in obese individuals, might be a critical pathomechanistic link. The objective of this study was to examine the impact of leptin on the regenerative capacity of human periodontal ligament (PDL) cells and also to study the local leptin production by these cells. Leptin caused a significant downregulation of growth (TGFβ1, and VEGFA) and transcription (RUNX2) factors as well as matrix molecules (collagen, and periostin) and inhibited SMAD signaling under regenerative conditions. Moreover, the local expression of leptin and its full-length receptor was significantly downregulated by inflammatory, microbial, and biomechanical signals. This study demonstrates that the hormone leptin negatively interferes with the regenerative capacity of PDL cells, suggesting leptin as a pathomechanistic link between obesity and compromised periodontal healing.http://dx.doi.org/10.1155/2014/180304
spellingShingle Marjan Nokhbehsaim
Sema Keser
Andressa Vilas Boas Nogueira
Andreas Jäger
Søren Jepsen
Joni Augusto Cirelli
Christoph Bourauel
Sigrun Eick
James Deschner
Leptin Effects on the Regenerative Capacity of Human Periodontal Cells
International Journal of Endocrinology
title Leptin Effects on the Regenerative Capacity of Human Periodontal Cells
title_full Leptin Effects on the Regenerative Capacity of Human Periodontal Cells
title_fullStr Leptin Effects on the Regenerative Capacity of Human Periodontal Cells
title_full_unstemmed Leptin Effects on the Regenerative Capacity of Human Periodontal Cells
title_short Leptin Effects on the Regenerative Capacity of Human Periodontal Cells
title_sort leptin effects on the regenerative capacity of human periodontal cells
url http://dx.doi.org/10.1155/2014/180304
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AT sørenjepsen leptineffectsontheregenerativecapacityofhumanperiodontalcells
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