In vitro evaluation of Punica granatum fruit peel extract for its potential anti-diabetic effects
Background: With growing awareness of pomegranate's health benefits, pomegranate products have been consumed more frequently in recent years, and pomegranate peel has emerged as one of the most prevalent wastes in the food industry. Pomegranate Peel concentrate is an indigenous substance with s...
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Creative Pharma Assent
2024-12-01
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| Series: | Journal of Applied Pharmaceutical Research |
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| Online Access: | https://japtronline.com/index.php/joapr/article/view/739 |
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| author | Rutuja K More Prashant L Pingale Chandrashekhar D Upasani Sunil V Amrutkar |
| author_facet | Rutuja K More Prashant L Pingale Chandrashekhar D Upasani Sunil V Amrutkar |
| author_sort | Rutuja K More |
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| description | Background: With growing awareness of pomegranate's health benefits, pomegranate products have been consumed more frequently in recent years, and pomegranate peel has emerged as one of the most prevalent wastes in the food industry. Pomegranate Peel concentrate is an indigenous substance with strong antioxidant and antidiabetic actions as a result of its tannins as well as polyphenols content. Methods: In the present study, pomegranate skin extract, both liquid and alcohol-based, was evaluated for polyphenolic and flavonoid content. Alcoholic fruit peel extract was also assessed for 1,4-α-D-glucan glucanohydrolase and α-D-glucoside glucohydrolase enzyme activity. Results: According to findings, pomegranate peel extract showed significant antioxidant content. Phytochemical analysis of ethanol-derived extract of pomegranate peel found a noteworthy amount of ellagitannins and flavonoids such as Punicalin, Punicalgin, Punicic acid, Catechin, Quercetin, Rutin, and Kaempferol. In contrast, punicalgin, ellagic acid, and gallic acid are responsible for antidiabetic activity. The LC-MS characterization of peel extract of pomegranate showed 10 bioactive compounds. The IC50 value for 80% alcoholic extract of pomegranate peel was found to be 5.86 mg/ml of α- amylase and 6.58 mg/ml of α-glucosidase. Conclusion: It was found that the inhibition of 1,4-α-D-glucan glucanohydrolase and α-D-glucoside glucohydrolase enzymes could be an effective mechanism by which it can give anti-diabetic effects. |
| format | Article |
| id | doaj-art-b7d88f97cfe64bd2948a96baaa56b32e |
| institution | Kabale University |
| issn | 2348-0335 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Creative Pharma Assent |
| record_format | Article |
| series | Journal of Applied Pharmaceutical Research |
| spelling | doaj-art-b7d88f97cfe64bd2948a96baaa56b32e2025-01-01T17:47:42ZengCreative Pharma AssentJournal of Applied Pharmaceutical Research2348-03352024-12-0112613714310.69857/joapr.v12i6.739740In vitro evaluation of Punica granatum fruit peel extract for its potential anti-diabetic effectsRutuja K More0Prashant L Pingale1Chandrashekhar D Upasani2Sunil V Amrutkar3Department of Pharmaceutics, GES’s sir Dr. M. S. Gosavi College of Pharmaceutical Education and Research, Nashik Maharashtra, 422005Department of Pharmaceutics, GES’s sir Dr. M. S. Gosavi College of Pharmaceutical Education and Research, Nashik Maharashtra, 422005Department of Pharmacology, SNJB’s Shriman Sureshdada Jain College of Pharmacy, Neminagar, Chandwad, Maharashtra, 423101Department of Pharmaceutical Chemistry, GES’s sir Dr. M. S. Gosavi College of Pharmaceutical Education and Research, Nashik Maharashtra, 422005Background: With growing awareness of pomegranate's health benefits, pomegranate products have been consumed more frequently in recent years, and pomegranate peel has emerged as one of the most prevalent wastes in the food industry. Pomegranate Peel concentrate is an indigenous substance with strong antioxidant and antidiabetic actions as a result of its tannins as well as polyphenols content. Methods: In the present study, pomegranate skin extract, both liquid and alcohol-based, was evaluated for polyphenolic and flavonoid content. Alcoholic fruit peel extract was also assessed for 1,4-α-D-glucan glucanohydrolase and α-D-glucoside glucohydrolase enzyme activity. Results: According to findings, pomegranate peel extract showed significant antioxidant content. Phytochemical analysis of ethanol-derived extract of pomegranate peel found a noteworthy amount of ellagitannins and flavonoids such as Punicalin, Punicalgin, Punicic acid, Catechin, Quercetin, Rutin, and Kaempferol. In contrast, punicalgin, ellagic acid, and gallic acid are responsible for antidiabetic activity. The LC-MS characterization of peel extract of pomegranate showed 10 bioactive compounds. The IC50 value for 80% alcoholic extract of pomegranate peel was found to be 5.86 mg/ml of α- amylase and 6.58 mg/ml of α-glucosidase. Conclusion: It was found that the inhibition of 1,4-α-D-glucan glucanohydrolase and α-D-glucoside glucohydrolase enzymes could be an effective mechanism by which it can give anti-diabetic effects.https://japtronline.com/index.php/joapr/article/view/739punica granatumsoxhlet extractionbioactives1,4-α-d-glucan glucanohydrolaseα-d-glucoside glucohydrolase enzymes |
| spellingShingle | Rutuja K More Prashant L Pingale Chandrashekhar D Upasani Sunil V Amrutkar In vitro evaluation of Punica granatum fruit peel extract for its potential anti-diabetic effects Journal of Applied Pharmaceutical Research punica granatum soxhlet extraction bioactives 1,4-α-d-glucan glucanohydrolase α-d-glucoside glucohydrolase enzymes |
| title | In vitro evaluation of Punica granatum fruit peel extract for its potential anti-diabetic effects |
| title_full | In vitro evaluation of Punica granatum fruit peel extract for its potential anti-diabetic effects |
| title_fullStr | In vitro evaluation of Punica granatum fruit peel extract for its potential anti-diabetic effects |
| title_full_unstemmed | In vitro evaluation of Punica granatum fruit peel extract for its potential anti-diabetic effects |
| title_short | In vitro evaluation of Punica granatum fruit peel extract for its potential anti-diabetic effects |
| title_sort | in vitro evaluation of punica granatum fruit peel extract for its potential anti diabetic effects |
| topic | punica granatum soxhlet extraction bioactives 1,4-α-d-glucan glucanohydrolase α-d-glucoside glucohydrolase enzymes |
| url | https://japtronline.com/index.php/joapr/article/view/739 |
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