Protocol to evaluate fasting metabolism and its relationship to the core circadian clock in mice
Summary: To investigate the role of hepatocyte genes in fasting metabolism, it is essential to analyze their expression across the entire 24-h circadian cycle. Here, we present a protocol to evaluate fasting metabolism and its relationship to the core circadian clock in mice. We describe steps for t...
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| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725000668 |
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| author | Jiameng Sun Brian J. DeBosch |
| author_facet | Jiameng Sun Brian J. DeBosch |
| author_sort | Jiameng Sun |
| collection | DOAJ |
| description | Summary: To investigate the role of hepatocyte genes in fasting metabolism, it is essential to analyze their expression across the entire 24-h circadian cycle. Here, we present a protocol to evaluate fasting metabolism and its relationship to the core circadian clock in mice. We describe steps for time-course fasting/feeding experiment setup and performing staggered fasting and refeeding for 24 h. We then detail procedures for evaluating oxidative substrate selection and rescuing defective oxidative metabolism through FGF21 and CPI-613 injections.For complete details on the use and execution of this protocol, please refer to Sun et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-b7c4d3080b3d4184a38cfa29f13a7587 |
| institution | DOAJ |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-b7c4d3080b3d4184a38cfa29f13a75872025-08-20T03:11:06ZengElsevierSTAR Protocols2666-16672025-03-016110366010.1016/j.xpro.2025.103660Protocol to evaluate fasting metabolism and its relationship to the core circadian clock in miceJiameng Sun0Brian J. DeBosch1Department of Pediatrics, Washington University School of Medicine in St. Louis, St. Louis, MO 63110, USA; Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN 46202, USAHerman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN 46202, USA; Corresponding authorSummary: To investigate the role of hepatocyte genes in fasting metabolism, it is essential to analyze their expression across the entire 24-h circadian cycle. Here, we present a protocol to evaluate fasting metabolism and its relationship to the core circadian clock in mice. We describe steps for time-course fasting/feeding experiment setup and performing staggered fasting and refeeding for 24 h. We then detail procedures for evaluating oxidative substrate selection and rescuing defective oxidative metabolism through FGF21 and CPI-613 injections.For complete details on the use and execution of this protocol, please refer to Sun et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725000668geneticsmetabolismmodel organisms |
| spellingShingle | Jiameng Sun Brian J. DeBosch Protocol to evaluate fasting metabolism and its relationship to the core circadian clock in mice STAR Protocols genetics metabolism model organisms |
| title | Protocol to evaluate fasting metabolism and its relationship to the core circadian clock in mice |
| title_full | Protocol to evaluate fasting metabolism and its relationship to the core circadian clock in mice |
| title_fullStr | Protocol to evaluate fasting metabolism and its relationship to the core circadian clock in mice |
| title_full_unstemmed | Protocol to evaluate fasting metabolism and its relationship to the core circadian clock in mice |
| title_short | Protocol to evaluate fasting metabolism and its relationship to the core circadian clock in mice |
| title_sort | protocol to evaluate fasting metabolism and its relationship to the core circadian clock in mice |
| topic | genetics metabolism model organisms |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725000668 |
| work_keys_str_mv | AT jiamengsun protocoltoevaluatefastingmetabolismanditsrelationshiptothecorecircadianclockinmice AT brianjdebosch protocoltoevaluatefastingmetabolismanditsrelationshiptothecorecircadianclockinmice |