Regenerative and Anti-Senescence Potential of Extracts from Different Parts of Black Persimmon in an In Vitro Model of Vascular Endothelium
Antioxidants are essential for mitigating oxidative stress and maintaining vascular health. Endothelial colony-forming cells (ECFCs) are pivotal in endothelial regeneration and angiogenesis and serve as a model to study the diversity of endothelial cells across various organs. This study evaluated t...
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2024-10-01
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| author | Graziella Serio Sina Naserian Sawssen Ben Fraj Georges Uzan Carla Gentile |
| author_facet | Graziella Serio Sina Naserian Sawssen Ben Fraj Georges Uzan Carla Gentile |
| author_sort | Graziella Serio |
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| description | Antioxidants are essential for mitigating oxidative stress and maintaining vascular health. Endothelial colony-forming cells (ECFCs) are pivotal in endothelial regeneration and angiogenesis and serve as a model to study the diversity of endothelial cells across various organs. This study evaluated the effects of peel, pulp, and seed extracts from <i>Diospyros digyna</i> Jacq. fruit (black persimmon) on human cord blood-derived ECFCs (CB-ECFCs) to determine how the distinct antioxidant profiles of the fruit’s different parts influence cellular functions. The extracts did not affect endothelial marker expression, cell proliferation, or nitric oxide production, indicating no cytotoxic or inflammatory effects. However, functional assays revealed that the seed extract significantly enhanced tube formation, increasing closed tubular networks by 1.5-fold. All extracts promoted cell migration, with the seed extract demonstrating the most substantial effect, surpassing even vascular endothelial growth factor (VEGF). Additionally, the seed extract exhibited the strongest reduction in cellular senescence, both before and after oxidative stress induction with H<sub>2</sub>O<sub>2</sub>. These findings underscore the potential of black persimmon extracts, especially from the seed, to enhance the regenerative capabilities of CB-ECFCs and reduce cellular senescence without affecting the normal endothelial phenotype. This positions them as promising candidates for developing endothelial cell therapies and advancing vascular regeneration. |
| format | Article |
| id | doaj-art-b7bf69f00b5143ce9771916c302bbcd3 |
| institution | DOAJ |
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| language | English |
| publishDate | 2024-10-01 |
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| series | Foods |
| spelling | doaj-art-b7bf69f00b5143ce9771916c302bbcd32025-08-20T02:49:56ZengMDPI AGFoods2304-81582024-10-011321336610.3390/foods13213366Regenerative and Anti-Senescence Potential of Extracts from Different Parts of Black Persimmon in an In Vitro Model of Vascular EndotheliumGraziella Serio0Sina Naserian1Sawssen Ben Fraj2Georges Uzan3Carla Gentile4Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, University of Palermo, Viale delle Scienze, 90128 Palermo, ItalyInstitut National de la Santé Et de la Recherche Médicale (INSERM) UMR-S-MD 1197, Hôpital Paul Brousse, 94800 Villejuif, FranceInstitut National de la Santé Et de la Recherche Médicale (INSERM) UMR-S-MD 1197, Hôpital Paul Brousse, 94800 Villejuif, FranceInstitut National de la Santé Et de la Recherche Médicale (INSERM) UMR-S-MD 1197, Hôpital Paul Brousse, 94800 Villejuif, FranceDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies, University of Palermo, Viale delle Scienze, 90128 Palermo, ItalyAntioxidants are essential for mitigating oxidative stress and maintaining vascular health. Endothelial colony-forming cells (ECFCs) are pivotal in endothelial regeneration and angiogenesis and serve as a model to study the diversity of endothelial cells across various organs. This study evaluated the effects of peel, pulp, and seed extracts from <i>Diospyros digyna</i> Jacq. fruit (black persimmon) on human cord blood-derived ECFCs (CB-ECFCs) to determine how the distinct antioxidant profiles of the fruit’s different parts influence cellular functions. The extracts did not affect endothelial marker expression, cell proliferation, or nitric oxide production, indicating no cytotoxic or inflammatory effects. However, functional assays revealed that the seed extract significantly enhanced tube formation, increasing closed tubular networks by 1.5-fold. All extracts promoted cell migration, with the seed extract demonstrating the most substantial effect, surpassing even vascular endothelial growth factor (VEGF). Additionally, the seed extract exhibited the strongest reduction in cellular senescence, both before and after oxidative stress induction with H<sub>2</sub>O<sub>2</sub>. These findings underscore the potential of black persimmon extracts, especially from the seed, to enhance the regenerative capabilities of CB-ECFCs and reduce cellular senescence without affecting the normal endothelial phenotype. This positions them as promising candidates for developing endothelial cell therapies and advancing vascular regeneration.https://www.mdpi.com/2304-8158/13/21/3366bioactive compoundsantioxidantsendothelial progenitor cellscellular senescence<i>Diospyros digyna</i> Jacq. |
| spellingShingle | Graziella Serio Sina Naserian Sawssen Ben Fraj Georges Uzan Carla Gentile Regenerative and Anti-Senescence Potential of Extracts from Different Parts of Black Persimmon in an In Vitro Model of Vascular Endothelium Foods bioactive compounds antioxidants endothelial progenitor cells cellular senescence <i>Diospyros digyna</i> Jacq. |
| title | Regenerative and Anti-Senescence Potential of Extracts from Different Parts of Black Persimmon in an In Vitro Model of Vascular Endothelium |
| title_full | Regenerative and Anti-Senescence Potential of Extracts from Different Parts of Black Persimmon in an In Vitro Model of Vascular Endothelium |
| title_fullStr | Regenerative and Anti-Senescence Potential of Extracts from Different Parts of Black Persimmon in an In Vitro Model of Vascular Endothelium |
| title_full_unstemmed | Regenerative and Anti-Senescence Potential of Extracts from Different Parts of Black Persimmon in an In Vitro Model of Vascular Endothelium |
| title_short | Regenerative and Anti-Senescence Potential of Extracts from Different Parts of Black Persimmon in an In Vitro Model of Vascular Endothelium |
| title_sort | regenerative and anti senescence potential of extracts from different parts of black persimmon in an in vitro model of vascular endothelium |
| topic | bioactive compounds antioxidants endothelial progenitor cells cellular senescence <i>Diospyros digyna</i> Jacq. |
| url | https://www.mdpi.com/2304-8158/13/21/3366 |
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