Amplicon-based metagenomic survey of microbes associated with the organic and inorganic rhizosphere soil of Glycine max L.

Abstract Objectives The metagenomic dataset of 16S rRNA and ITS gene amplicons of DNA were obtained from the cultivated soybean rhizosphere of organic and inorganic treatments. The organic treatments consisted of poultry waste, and cow dung treatments while the inorganic consisted of samples from un...

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Main Authors: Olubukola Oluranti Babalola, Ijeoma Emelda Osuji, Akinlolu Olalekan Akanmu
Format: Article
Language:English
Published: BMC 2025-06-01
Series:BMC Genomic Data
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Online Access:https://doi.org/10.1186/s12863-025-01333-2
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author Olubukola Oluranti Babalola
Ijeoma Emelda Osuji
Akinlolu Olalekan Akanmu
author_facet Olubukola Oluranti Babalola
Ijeoma Emelda Osuji
Akinlolu Olalekan Akanmu
author_sort Olubukola Oluranti Babalola
collection DOAJ
description Abstract Objectives The metagenomic dataset of 16S rRNA and ITS gene amplicons of DNA were obtained from the cultivated soybean rhizosphere of organic and inorganic treatments. The organic treatments consisted of poultry waste, and cow dung treatments while the inorganic consisted of samples from untreated soybean plots and the bulk. Amplicon sequencing was performed on the Illumina platform, and the raw sequence data were processed and analyzed using Quantitative Insights Into Microbial Ecology (QIIME 2 version 2019.1.). Data description The analysis revealed a metagenomic library from soybean rhizospheric soils, providing insights into diversity and distribution of the bacterial and fungal community diversities. The most predominant bacteria phylum taxa across the treatments were Proteobacteria, Firmicutes, Actinobacteriota and Bacteriodota, while those for fungi were Ascomycota, Basidiomycota and Glomeromycota. The dataset provides insights into how different organic fertilization sources affect the structure, composition, and diversity of the microbiome in the soybean rhizosphere. The sequences have been deposited in the Sequence Read Archive (SRA) of the National Center for Biotechnology Information (NCBI) with assigned bioproject accession numbers; 16S rRNA (SRP540791) and ITS (SRP541849).
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publishDate 2025-06-01
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spelling doaj-art-b7b34ae9f4d344e9bb9b300b1537efac2025-08-20T02:30:59ZengBMCBMC Genomic Data2730-68442025-06-012611310.1186/s12863-025-01333-2Amplicon-based metagenomic survey of microbes associated with the organic and inorganic rhizosphere soil of Glycine max L.Olubukola Oluranti Babalola0Ijeoma Emelda Osuji1Akinlolu Olalekan Akanmu2Food Security and Safety Focus Area, Faculty of Natural and Agricultural Sciences, North-West UniversityFood Security and Safety Focus Area, Faculty of Natural and Agricultural Sciences, North-West UniversityFood Security and Safety Focus Area, Faculty of Natural and Agricultural Sciences, North-West UniversityAbstract Objectives The metagenomic dataset of 16S rRNA and ITS gene amplicons of DNA were obtained from the cultivated soybean rhizosphere of organic and inorganic treatments. The organic treatments consisted of poultry waste, and cow dung treatments while the inorganic consisted of samples from untreated soybean plots and the bulk. Amplicon sequencing was performed on the Illumina platform, and the raw sequence data were processed and analyzed using Quantitative Insights Into Microbial Ecology (QIIME 2 version 2019.1.). Data description The analysis revealed a metagenomic library from soybean rhizospheric soils, providing insights into diversity and distribution of the bacterial and fungal community diversities. The most predominant bacteria phylum taxa across the treatments were Proteobacteria, Firmicutes, Actinobacteriota and Bacteriodota, while those for fungi were Ascomycota, Basidiomycota and Glomeromycota. The dataset provides insights into how different organic fertilization sources affect the structure, composition, and diversity of the microbiome in the soybean rhizosphere. The sequences have been deposited in the Sequence Read Archive (SRA) of the National Center for Biotechnology Information (NCBI) with assigned bioproject accession numbers; 16S rRNA (SRP540791) and ITS (SRP541849).https://doi.org/10.1186/s12863-025-01333-216S rRNA geneAmplicon sequencingCow dungITS geneMetagenomeMicrobial communities
spellingShingle Olubukola Oluranti Babalola
Ijeoma Emelda Osuji
Akinlolu Olalekan Akanmu
Amplicon-based metagenomic survey of microbes associated with the organic and inorganic rhizosphere soil of Glycine max L.
BMC Genomic Data
16S rRNA gene
Amplicon sequencing
Cow dung
ITS gene
Metagenome
Microbial communities
title Amplicon-based metagenomic survey of microbes associated with the organic and inorganic rhizosphere soil of Glycine max L.
title_full Amplicon-based metagenomic survey of microbes associated with the organic and inorganic rhizosphere soil of Glycine max L.
title_fullStr Amplicon-based metagenomic survey of microbes associated with the organic and inorganic rhizosphere soil of Glycine max L.
title_full_unstemmed Amplicon-based metagenomic survey of microbes associated with the organic and inorganic rhizosphere soil of Glycine max L.
title_short Amplicon-based metagenomic survey of microbes associated with the organic and inorganic rhizosphere soil of Glycine max L.
title_sort amplicon based metagenomic survey of microbes associated with the organic and inorganic rhizosphere soil of glycine max l
topic 16S rRNA gene
Amplicon sequencing
Cow dung
ITS gene
Metagenome
Microbial communities
url https://doi.org/10.1186/s12863-025-01333-2
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