Overexpression of the Kiwifruit Transcription Factor <i>AaMYB44</i> Decreases the Cold Tolerance in <i>Arabidopsis thaliana</i>
Cold stress is one of the main abiotic stresses that affect the development and growth of kiwifruit (<i>Actinidia arguta</i>). Herein, we analyzed the transcriptomic data of <i>A. arguta</i> dormant shoots in response to low-temperature treatment, identified 52 MYB genes, and...
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2024-11-01
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| author | Yihang Li Miaomiao Lin Qina Zhang Peng Zhang Zhenzhen Zhang Yukuo Li Leiming Sun Sumei Li Congcong Li Dixin Chen Xiujuan Qi |
| author_facet | Yihang Li Miaomiao Lin Qina Zhang Peng Zhang Zhenzhen Zhang Yukuo Li Leiming Sun Sumei Li Congcong Li Dixin Chen Xiujuan Qi |
| author_sort | Yihang Li |
| collection | DOAJ |
| description | Cold stress is one of the main abiotic stresses that affect the development and growth of kiwifruit (<i>Actinidia arguta</i>). Herein, we analyzed the transcriptomic data of <i>A. arguta</i> dormant shoots in response to low-temperature treatment, identified 52 MYB genes, and constructed a phylogenetic tree based on the encoded protein sequences. Then, the effect of one <i>MYB</i> gene on cold tolerance was analyzed. This gene had an open reading frame of 837 bp long and encoded 279 amino acids. Sequence alignment and phylogenetic analysis revealed that this gene belongs to the <i>R2R3-MYB</i> family and was named <i>AaMYB44</i> based on its homology to other <i>MYB</i> family members. Quantitative real-time PCR revealed that <i>AaMYB44</i> expression was significantly induced by low temperatures but exhibited the opposite trend in cold-tolerant genotypes. Subcellular localization assays revealed the nuclear localization of the AaMYB44 protein. Furthermore, <i>AaMYB44</i> was transformed into <i>Arabidopsis thaliana</i> (<i>A. thaliana</i>) via inflorescence infection, and physiological and biochemical tests revealed that the cold resistance and antioxidant capacity of the transgenic <i>A. thaliana</i> were lower than those of wild-type plants. Overall, <i>AaMYB44</i> might play a negative regulatory role in response to cold stress, providing new insight into the mechanism of cold tolerance. |
| format | Article |
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| publishDate | 2024-11-01 |
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| series | Plants |
| spelling | doaj-art-b7b186d2ffdf4c6ab4a6773dac27502c2025-08-20T02:05:01ZengMDPI AGPlants2223-77472024-11-011322312610.3390/plants13223126Overexpression of the Kiwifruit Transcription Factor <i>AaMYB44</i> Decreases the Cold Tolerance in <i>Arabidopsis thaliana</i>Yihang Li0Miaomiao Lin1Qina Zhang2Peng Zhang3Zhenzhen Zhang4Yukuo Li5Leiming Sun6Sumei Li7Congcong Li8Dixin Chen9Xiujuan Qi10College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang 471000, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaCollege of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang 471000, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaCold stress is one of the main abiotic stresses that affect the development and growth of kiwifruit (<i>Actinidia arguta</i>). Herein, we analyzed the transcriptomic data of <i>A. arguta</i> dormant shoots in response to low-temperature treatment, identified 52 MYB genes, and constructed a phylogenetic tree based on the encoded protein sequences. Then, the effect of one <i>MYB</i> gene on cold tolerance was analyzed. This gene had an open reading frame of 837 bp long and encoded 279 amino acids. Sequence alignment and phylogenetic analysis revealed that this gene belongs to the <i>R2R3-MYB</i> family and was named <i>AaMYB44</i> based on its homology to other <i>MYB</i> family members. Quantitative real-time PCR revealed that <i>AaMYB44</i> expression was significantly induced by low temperatures but exhibited the opposite trend in cold-tolerant genotypes. Subcellular localization assays revealed the nuclear localization of the AaMYB44 protein. Furthermore, <i>AaMYB44</i> was transformed into <i>Arabidopsis thaliana</i> (<i>A. thaliana</i>) via inflorescence infection, and physiological and biochemical tests revealed that the cold resistance and antioxidant capacity of the transgenic <i>A. thaliana</i> were lower than those of wild-type plants. Overall, <i>AaMYB44</i> might play a negative regulatory role in response to cold stress, providing new insight into the mechanism of cold tolerance.https://www.mdpi.com/2223-7747/13/22/3126kiwifruit<i>AaMYB44</i>transcriptomemechanism of freezing tolerance |
| spellingShingle | Yihang Li Miaomiao Lin Qina Zhang Peng Zhang Zhenzhen Zhang Yukuo Li Leiming Sun Sumei Li Congcong Li Dixin Chen Xiujuan Qi Overexpression of the Kiwifruit Transcription Factor <i>AaMYB44</i> Decreases the Cold Tolerance in <i>Arabidopsis thaliana</i> Plants kiwifruit <i>AaMYB44</i> transcriptome mechanism of freezing tolerance |
| title | Overexpression of the Kiwifruit Transcription Factor <i>AaMYB44</i> Decreases the Cold Tolerance in <i>Arabidopsis thaliana</i> |
| title_full | Overexpression of the Kiwifruit Transcription Factor <i>AaMYB44</i> Decreases the Cold Tolerance in <i>Arabidopsis thaliana</i> |
| title_fullStr | Overexpression of the Kiwifruit Transcription Factor <i>AaMYB44</i> Decreases the Cold Tolerance in <i>Arabidopsis thaliana</i> |
| title_full_unstemmed | Overexpression of the Kiwifruit Transcription Factor <i>AaMYB44</i> Decreases the Cold Tolerance in <i>Arabidopsis thaliana</i> |
| title_short | Overexpression of the Kiwifruit Transcription Factor <i>AaMYB44</i> Decreases the Cold Tolerance in <i>Arabidopsis thaliana</i> |
| title_sort | overexpression of the kiwifruit transcription factor i aamyb44 i decreases the cold tolerance in i arabidopsis thaliana i |
| topic | kiwifruit <i>AaMYB44</i> transcriptome mechanism of freezing tolerance |
| url | https://www.mdpi.com/2223-7747/13/22/3126 |
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