AG-NGS: A powerful and user-friendly computing application for the semi-automated preparation of next-generation sequencing libraries using open liquid handling platforms
Next-generation sequencing (NGS) is becoming one of the most widely used technologies in the field of genomics. Library preparation is one of the most critical, hands-on, and time-consuming steps in the NGS workflow. Each library must be prepared in an independent well, increasing the number of hour...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2014-01-01
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| Series: | BioTechniques |
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| Online Access: | https://www.future-science.com/doi/10.2144/000114124 |
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| author | Sergio Callejas Rebeca Álvarez Alberto Benguria Ana Dopazo |
| author_facet | Sergio Callejas Rebeca Álvarez Alberto Benguria Ana Dopazo |
| author_sort | Sergio Callejas |
| collection | DOAJ |
| description | Next-generation sequencing (NGS) is becoming one of the most widely used technologies in the field of genomics. Library preparation is one of the most critical, hands-on, and time-consuming steps in the NGS workflow. Each library must be prepared in an independent well, increasing the number of hours required for a sequencing run and the risk of human-introduced error. Automation of library preparation is the best option to avoid these problems. With this in mind, we have developed automatic genomics NGS (AG-NGS), a computing application that allows an open liquid handling platform to be transformed into a library preparation station without losing the potential of an open platform. Implementation of AG-NGS does not require programming experience, and the application has also been designed to minimize implementation costs. Automated library preparation with AG-NGS generated high-quality libraries from different samples, demonstrating its efficiency, and all quality control parameters fell within the range of optimal values. |
| format | Article |
| id | doaj-art-b7afaa3d1fda4544b73bdaea6e5d700e |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-b7afaa3d1fda4544b73bdaea6e5d700e2025-08-20T02:25:55ZengTaylor & Francis GroupBioTechniques0736-62051940-98182014-01-01561283510.2144/000114124AG-NGS: A powerful and user-friendly computing application for the semi-automated preparation of next-generation sequencing libraries using open liquid handling platformsSergio Callejas0Rebeca Álvarez1Alberto Benguria2Ana Dopazo31Spanish National Center for Cardiovascular Research (CNIC), Madrid, Spain1Spanish National Center for Cardiovascular Research (CNIC), Madrid, Spain1Spanish National Center for Cardiovascular Research (CNIC), Madrid, Spain1Spanish National Center for Cardiovascular Research (CNIC), Madrid, SpainNext-generation sequencing (NGS) is becoming one of the most widely used technologies in the field of genomics. Library preparation is one of the most critical, hands-on, and time-consuming steps in the NGS workflow. Each library must be prepared in an independent well, increasing the number of hours required for a sequencing run and the risk of human-introduced error. Automation of library preparation is the best option to avoid these problems. With this in mind, we have developed automatic genomics NGS (AG-NGS), a computing application that allows an open liquid handling platform to be transformed into a library preparation station without losing the potential of an open platform. Implementation of AG-NGS does not require programming experience, and the application has also been designed to minimize implementation costs. Automated library preparation with AG-NGS generated high-quality libraries from different samples, demonstrating its efficiency, and all quality control parameters fell within the range of optimal values.https://www.future-science.com/doi/10.2144/000114124next-generation sequencingsample preparationautomationliquid handling robotsoftware |
| spellingShingle | Sergio Callejas Rebeca Álvarez Alberto Benguria Ana Dopazo AG-NGS: A powerful and user-friendly computing application for the semi-automated preparation of next-generation sequencing libraries using open liquid handling platforms BioTechniques next-generation sequencing sample preparation automation liquid handling robot software |
| title | AG-NGS: A powerful and user-friendly computing application for the semi-automated preparation of next-generation sequencing libraries using open liquid handling platforms |
| title_full | AG-NGS: A powerful and user-friendly computing application for the semi-automated preparation of next-generation sequencing libraries using open liquid handling platforms |
| title_fullStr | AG-NGS: A powerful and user-friendly computing application for the semi-automated preparation of next-generation sequencing libraries using open liquid handling platforms |
| title_full_unstemmed | AG-NGS: A powerful and user-friendly computing application for the semi-automated preparation of next-generation sequencing libraries using open liquid handling platforms |
| title_short | AG-NGS: A powerful and user-friendly computing application for the semi-automated preparation of next-generation sequencing libraries using open liquid handling platforms |
| title_sort | ag ngs a powerful and user friendly computing application for the semi automated preparation of next generation sequencing libraries using open liquid handling platforms |
| topic | next-generation sequencing sample preparation automation liquid handling robot software |
| url | https://www.future-science.com/doi/10.2144/000114124 |
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