Blending enzyme immobilization enabled high performance glucose sensor based on an n-channel organic electrochemical transistor

Organic electrochemical transistors (OECTs) have emerged as an advantageous choice for constructing biosensors due to their remarkable water compatibility, low operating voltage, and inherent amplification capability. However, the current research on bio-sensing based on OECTs predominantly employs...

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Main Authors: Cheng Shi, Xingyu Jiang, Qi Wang, Chuan Xiang, Xinyu Dong, Lifeng Chi, Lizhen Huang
Format: Article
Language:English
Published: IOP Publishing 2024-01-01
Series:Materials Research Express
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Online Access:https://doi.org/10.1088/2053-1591/ad95e1
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author Cheng Shi
Xingyu Jiang
Qi Wang
Chuan Xiang
Xinyu Dong
Lifeng Chi
Lizhen Huang
author_facet Cheng Shi
Xingyu Jiang
Qi Wang
Chuan Xiang
Xinyu Dong
Lifeng Chi
Lizhen Huang
author_sort Cheng Shi
collection DOAJ
description Organic electrochemical transistors (OECTs) have emerged as an advantageous choice for constructing biosensors due to their remarkable water compatibility, low operating voltage, and inherent amplification capability. However, the current research on bio-sensing based on OECTs predominantly employs p–type material PEDOT:PSS as the channel material. Nevertheless, the utilization of a single material and its depleting characteristics impose significant limitations on device miniaturization and integration applications. We present a high-sensitivity glucose sensor based on n-channel accumulation-type OECT through immobilization the glucose oxidase on Pt gate electrode with an blending approach. The glucose oxidase directly mixed with bovine serum albumin, chitosan and immolizied on the gate electode, demonstrating a stable and sensitive response to the glucose. The modified n-channel organic electrochemical transistor demonstrates a highly sensitive response to glucose across a concentration range from μ M to mM, with a quantified stable sensitivity over 2.69 mmol ^–1 by normalizing the current change with respect to concentration. The device also exhibits selectivity towards glucose (compared with high concentrations of lactic acid, different concentration gradients of ascorbic acid, and uric acid), rendering it suitable for noninvasive glucose detection in body fluid like sweat, saliva etc This flexible and ensitive electrochemical transistor sensor holds immense potential for the development of potable healthcare biosensing.
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spelling doaj-art-b7a14e8d2c3b446c9ecc55a7e17fc2bb2025-08-20T02:07:10ZengIOP PublishingMaterials Research Express2053-15912024-01-01111111590310.1088/2053-1591/ad95e1Blending enzyme immobilization enabled high performance glucose sensor based on an n-channel organic electrochemical transistorCheng Shi0https://orcid.org/0009-0000-0973-3831Xingyu Jiang1Qi Wang2Chuan Xiang3Xinyu Dong4Lifeng Chi5Lizhen Huang6https://orcid.org/0000-0002-5211-921XInstitute of Functional Nano & Soft Materials (FUNSOM), Soochow University , 199 Ren’ai Road, Suzhou, 215123, Jiangsu, People’s Republic of ChinaInstitute of Functional Nano & Soft Materials (FUNSOM), Soochow University , 199 Ren’ai Road, Suzhou, 215123, Jiangsu, People’s Republic of ChinaInstitute of Functional Nano & Soft Materials (FUNSOM), Soochow University , 199 Ren’ai Road, Suzhou, 215123, Jiangsu, People’s Republic of ChinaInstitute of Functional Nano & Soft Materials (FUNSOM), Soochow University , 199 Ren’ai Road, Suzhou, 215123, Jiangsu, People’s Republic of ChinaInstitute of Functional Nano & Soft Materials (FUNSOM), Soochow University , 199 Ren’ai Road, Suzhou, 215123, Jiangsu, People’s Republic of ChinaInstitute of Functional Nano & Soft Materials (FUNSOM), Soochow University , 199 Ren’ai Road, Suzhou, 215123, Jiangsu, People’s Republic of China; Macao Institute of Materials Science and Engineering (MIMSE), MUST-SUDA Joint Research Center for Advanced Functional Materials, Macau University of Science and Technology , Taipa 999078, Macao, People’s Republic of ChinaInstitute of Functional Nano & Soft Materials (FUNSOM), Soochow University , 199 Ren’ai Road, Suzhou, 215123, Jiangsu, People’s Republic of ChinaOrganic electrochemical transistors (OECTs) have emerged as an advantageous choice for constructing biosensors due to their remarkable water compatibility, low operating voltage, and inherent amplification capability. However, the current research on bio-sensing based on OECTs predominantly employs p–type material PEDOT:PSS as the channel material. Nevertheless, the utilization of a single material and its depleting characteristics impose significant limitations on device miniaturization and integration applications. We present a high-sensitivity glucose sensor based on n-channel accumulation-type OECT through immobilization the glucose oxidase on Pt gate electrode with an blending approach. The glucose oxidase directly mixed with bovine serum albumin, chitosan and immolizied on the gate electode, demonstrating a stable and sensitive response to the glucose. The modified n-channel organic electrochemical transistor demonstrates a highly sensitive response to glucose across a concentration range from μ M to mM, with a quantified stable sensitivity over 2.69 mmol ^–1 by normalizing the current change with respect to concentration. The device also exhibits selectivity towards glucose (compared with high concentrations of lactic acid, different concentration gradients of ascorbic acid, and uric acid), rendering it suitable for noninvasive glucose detection in body fluid like sweat, saliva etc This flexible and ensitive electrochemical transistor sensor holds immense potential for the development of potable healthcare biosensing.https://doi.org/10.1088/2053-1591/ad95e1n–type OECTglucose sensingelectrochemistryenzyme immobilization
spellingShingle Cheng Shi
Xingyu Jiang
Qi Wang
Chuan Xiang
Xinyu Dong
Lifeng Chi
Lizhen Huang
Blending enzyme immobilization enabled high performance glucose sensor based on an n-channel organic electrochemical transistor
Materials Research Express
n–type OECT
glucose sensing
electrochemistry
enzyme immobilization
title Blending enzyme immobilization enabled high performance glucose sensor based on an n-channel organic electrochemical transistor
title_full Blending enzyme immobilization enabled high performance glucose sensor based on an n-channel organic electrochemical transistor
title_fullStr Blending enzyme immobilization enabled high performance glucose sensor based on an n-channel organic electrochemical transistor
title_full_unstemmed Blending enzyme immobilization enabled high performance glucose sensor based on an n-channel organic electrochemical transistor
title_short Blending enzyme immobilization enabled high performance glucose sensor based on an n-channel organic electrochemical transistor
title_sort blending enzyme immobilization enabled high performance glucose sensor based on an n channel organic electrochemical transistor
topic n–type OECT
glucose sensing
electrochemistry
enzyme immobilization
url https://doi.org/10.1088/2053-1591/ad95e1
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AT xingyujiang blendingenzymeimmobilizationenabledhighperformanceglucosesensorbasedonannchannelorganicelectrochemicaltransistor
AT qiwang blendingenzymeimmobilizationenabledhighperformanceglucosesensorbasedonannchannelorganicelectrochemicaltransistor
AT chuanxiang blendingenzymeimmobilizationenabledhighperformanceglucosesensorbasedonannchannelorganicelectrochemicaltransistor
AT xinyudong blendingenzymeimmobilizationenabledhighperformanceglucosesensorbasedonannchannelorganicelectrochemicaltransistor
AT lifengchi blendingenzymeimmobilizationenabledhighperformanceglucosesensorbasedonannchannelorganicelectrochemicaltransistor
AT lizhenhuang blendingenzymeimmobilizationenabledhighperformanceglucosesensorbasedonannchannelorganicelectrochemicaltransistor