Lymph flow, ionic and biochemical indicators of lymph and blood during hypoxia
Abstract In this study, the biochemical parameters and physico-chemical reactions of the body in experimental hypoxia, using a Sprague Dawley Rat Model. Hypoxia changed the dynamics and biochemical parameters of blood and lymph, as well as urine. During hypoxia, there was a change in the osmotic res...
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Instituto Internacional de Ecologia
2025-02-01
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| Series: | Brazilian Journal of Biology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842024000101383&lng=en&tlng=en |
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| author | S. N. Abdreshov G. A. Demchenko U. N. Kozhaniyazova A. N. Yeshmukhanbet M. A. Yessenova B. A. Nurmakhanova R. M. Karjaubaev L. U. Koibasova |
| author_facet | S. N. Abdreshov G. A. Demchenko U. N. Kozhaniyazova A. N. Yeshmukhanbet M. A. Yessenova B. A. Nurmakhanova R. M. Karjaubaev L. U. Koibasova |
| author_sort | S. N. Abdreshov |
| collection | DOAJ |
| description | Abstract In this study, the biochemical parameters and physico-chemical reactions of the body in experimental hypoxia, using a Sprague Dawley Rat Model. Hypoxia changed the dynamics and biochemical parameters of blood and lymph, as well as urine. During hypoxia, there was a change in the osmotic resistance of erythrocytes. Hypoxic training was conducted in a hypoxic animal chamber for 15 days and 30 days for 40 minutes every day. Physical and chemical parameters of blood, lymph and its morphological composition were studied on a hematological analyser, oxygen tension and pH of blood and lymph on an OPTI CCA-TS2 Blood Gas and Electrolyte Analyser. The value of osmotic pressure in the lymph changed slightly from 280.22 ± 2.07 to 293.3±3.1 and 285.6 ± 2.8 mOsm/l, respectively, 15 and 30 days of hypoxia. Urine osmotic pressure decreased by 15.1-10.4%, respectively, compared to the control group. After 15 and 30 days of hypoxia, ion exchange in the blood plasma showed a decrease in the concentration of K+, Cl- ions and an increase in the concentration of Na+ ions in the blood plasma and lymph. Ca2+ concentrations decreased in blood plasma and increased in lymph and urine. The analysis of the osmotic resistance of erythrocytes showed its decrease. Lipid peroxidation of erythrocyte membranes showed a significant increase in the level of malondialdehyde and diene conjugates by 52.2% and 69.6%, as well as a decrease in the activity of superoxide dismutase and catalase by 32% and 29.7%. Hypoxia leads to a decrease in erythrocyte resistance and lipid peroxidation in experimental animals. Shifts in pH on the side of acidosis and disturbances in physico-chemical properties in the blood and lymph were detected. As a result of developing hypoxia in the body, structural and functional rearrangements occur in the whole blood of experimental animals. |
| format | Article |
| id | doaj-art-b79afc36b4f14702bd802a48e597a805 |
| institution | OA Journals |
| issn | 1678-4375 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Instituto Internacional de Ecologia |
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| series | Brazilian Journal of Biology |
| spelling | doaj-art-b79afc36b4f14702bd802a48e597a8052025-08-20T02:01:19ZengInstituto Internacional de EcologiaBrazilian Journal of Biology1678-43752025-02-018410.1590/1519-6984.284264Lymph flow, ionic and biochemical indicators of lymph and blood during hypoxiaS. N. Abdreshovhttps://orcid.org/0000-0002-8527-921XG. A. Demchenkohttps://orcid.org/0000-0001-9906-2700U. N. Kozhaniyazovahttps://orcid.org/0000-0001-6122-0320A. N. Yeshmukhanbethttps://orcid.org/0000-0002-8665-1458M. A. Yessenovahttps://orcid.org/0000-0002-2436-0620B. A. Nurmakhanovahttps://orcid.org/0000-0002-5923-3496R. M. Karjaubaevhttps://orcid.org/0009-0005-1248-1520L. U. Koibasovahttps://orcid.org/0000-0002-9898-3580Abstract In this study, the biochemical parameters and physico-chemical reactions of the body in experimental hypoxia, using a Sprague Dawley Rat Model. Hypoxia changed the dynamics and biochemical parameters of blood and lymph, as well as urine. During hypoxia, there was a change in the osmotic resistance of erythrocytes. Hypoxic training was conducted in a hypoxic animal chamber for 15 days and 30 days for 40 minutes every day. Physical and chemical parameters of blood, lymph and its morphological composition were studied on a hematological analyser, oxygen tension and pH of blood and lymph on an OPTI CCA-TS2 Blood Gas and Electrolyte Analyser. The value of osmotic pressure in the lymph changed slightly from 280.22 ± 2.07 to 293.3±3.1 and 285.6 ± 2.8 mOsm/l, respectively, 15 and 30 days of hypoxia. Urine osmotic pressure decreased by 15.1-10.4%, respectively, compared to the control group. After 15 and 30 days of hypoxia, ion exchange in the blood plasma showed a decrease in the concentration of K+, Cl- ions and an increase in the concentration of Na+ ions in the blood plasma and lymph. Ca2+ concentrations decreased in blood plasma and increased in lymph and urine. The analysis of the osmotic resistance of erythrocytes showed its decrease. Lipid peroxidation of erythrocyte membranes showed a significant increase in the level of malondialdehyde and diene conjugates by 52.2% and 69.6%, as well as a decrease in the activity of superoxide dismutase and catalase by 32% and 29.7%. Hypoxia leads to a decrease in erythrocyte resistance and lipid peroxidation in experimental animals. Shifts in pH on the side of acidosis and disturbances in physico-chemical properties in the blood and lymph were detected. As a result of developing hypoxia in the body, structural and functional rearrangements occur in the whole blood of experimental animals.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842024000101383&lng=en&tlng=enbloodhypoxiaionslymphmembranes |
| spellingShingle | S. N. Abdreshov G. A. Demchenko U. N. Kozhaniyazova A. N. Yeshmukhanbet M. A. Yessenova B. A. Nurmakhanova R. M. Karjaubaev L. U. Koibasova Lymph flow, ionic and biochemical indicators of lymph and blood during hypoxia Brazilian Journal of Biology blood hypoxia ions lymph membranes |
| title | Lymph flow, ionic and biochemical indicators of lymph and blood during hypoxia |
| title_full | Lymph flow, ionic and biochemical indicators of lymph and blood during hypoxia |
| title_fullStr | Lymph flow, ionic and biochemical indicators of lymph and blood during hypoxia |
| title_full_unstemmed | Lymph flow, ionic and biochemical indicators of lymph and blood during hypoxia |
| title_short | Lymph flow, ionic and biochemical indicators of lymph and blood during hypoxia |
| title_sort | lymph flow ionic and biochemical indicators of lymph and blood during hypoxia |
| topic | blood hypoxia ions lymph membranes |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842024000101383&lng=en&tlng=en |
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