Detection and characterization of palm lethal decline phytoplasmas, subgroups 16SrIV-A and -D, in Phoenix canariensis and Syagrus romanzoffiana in Puebla, Mexico

Phytoplasma subgroups 16SrIV-A and -D are the agents associated with two diseases that significantly threaten palm cultivation in the Americas, namely lethal yellowing (LY) and Texas Phoenix palm decline (TPPD), respectively. Recently, in Puebla State, Mexico, several Phoenix canariensis Chabaud and...

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Main Authors: Pablo José Palma-Cancino, Carlos Fredy Ortiz-García, Jesús Francisco López-Olguín, Agustín Aragón-García
Format: Article
Language:English
Published: Polish Academy of Sciences
Series:Journal of Plant Protection Research
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Online Access:https://journals.pan.pl/Content/134612/PDF/10_OA_JPPR_65_1_2035_Palmo-Cancina.pdf
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author Pablo José Palma-Cancino
Carlos Fredy Ortiz-García
Jesús Francisco López-Olguín
Agustín Aragón-García
author_facet Pablo José Palma-Cancino
Carlos Fredy Ortiz-García
Jesús Francisco López-Olguín
Agustín Aragón-García
author_sort Pablo José Palma-Cancino
collection DOAJ
description Phytoplasma subgroups 16SrIV-A and -D are the agents associated with two diseases that significantly threaten palm cultivation in the Americas, namely lethal yellowing (LY) and Texas Phoenix palm decline (TPPD), respectively. Recently, in Puebla State, Mexico, several Phoenix canariensis Chabaud and Syagrus romanzoffiana (Cham.) Glassman palms used as ornamentals began to show symptoms resembling those of TPPD and LY. Therefore, the present study aimed to demonstrate the spread of group 16SrIV phytoplasmas to Puebla, Mexico. Ten symptomatic individuals of both palms were sampled and a nested PCR assay with primer pair P1/P7 followed by LY16Sf/LY16Sr was performed to detect phytoplasma presence. A fragment of about 1.4 kb was amplified in six palms, three (of four) P. canar­iensis and three (of six) S. romanzoffiana. Sequence analysis of the amplicons revealed that the phytoplasma isolates from Puebla were members of group 16SrIV, subgroups – A (one isolate from P. canariensis) and -D (rest of isolates). This study reports the first occurrence of TPPD and LY on ornamental palm species in the state of Puebla, Mexico.
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spelling doaj-art-b795cd26ce244476abebb5065548764f2025-08-20T02:38:35ZengPolish Academy of SciencesJournal of Plant Protection Research1427-43451899-007Xvol. 65No 1https://doi.org/10.24425/jppr.2025.153823Detection and characterization of palm lethal decline phytoplasmas, subgroups 16SrIV-A and -D, in Phoenix canariensis and Syagrus romanzoffiana in Puebla, MexicoPablo José Palma-Cancino0https://orcid.org/0000-0003-4584-5464Carlos Fredy Ortiz-García1https://orcid.org/0000-0002-6820-9252Jesús Francisco López-Olguín2https://orcid.org/0000-0003-0209-5966Agustín Aragón-García3https://orcid.org/0000-0001-9801-6091Campus Tabasco, Postgraduate College, Cárdenas, Tabasco, MexicoCampus Tabasco, Postgraduate College, Cárdenas, Tabasco, MexicoHerbarium and Botanical Garden, Vice-rectorate for Research and Postgraduate Studies, Meritorious Autonomous University of Puebla, Puebla, MexicoAgroecology Center, Institute of Sciences, Meritorious Autonomous University of Puebla, Puebla, MexicoPhytoplasma subgroups 16SrIV-A and -D are the agents associated with two diseases that significantly threaten palm cultivation in the Americas, namely lethal yellowing (LY) and Texas Phoenix palm decline (TPPD), respectively. Recently, in Puebla State, Mexico, several Phoenix canariensis Chabaud and Syagrus romanzoffiana (Cham.) Glassman palms used as ornamentals began to show symptoms resembling those of TPPD and LY. Therefore, the present study aimed to demonstrate the spread of group 16SrIV phytoplasmas to Puebla, Mexico. Ten symptomatic individuals of both palms were sampled and a nested PCR assay with primer pair P1/P7 followed by LY16Sf/LY16Sr was performed to detect phytoplasma presence. A fragment of about 1.4 kb was amplified in six palms, three (of four) P. canar­iensis and three (of six) S. romanzoffiana. Sequence analysis of the amplicons revealed that the phytoplasma isolates from Puebla were members of group 16SrIV, subgroups – A (one isolate from P. canariensis) and -D (rest of isolates). This study reports the first occurrence of TPPD and LY on ornamental palm species in the state of Puebla, Mexico.https://journals.pan.pl/Content/134612/PDF/10_OA_JPPR_65_1_2035_Palmo-Cancina.pdflethal bronzinglethal yellowingornamental palmsphytoplasma diseasestexas phoenix palm decline
spellingShingle Pablo José Palma-Cancino
Carlos Fredy Ortiz-García
Jesús Francisco López-Olguín
Agustín Aragón-García
Detection and characterization of palm lethal decline phytoplasmas, subgroups 16SrIV-A and -D, in Phoenix canariensis and Syagrus romanzoffiana in Puebla, Mexico
Journal of Plant Protection Research
lethal bronzing
lethal yellowing
ornamental palms
phytoplasma diseases
texas phoenix palm decline
title Detection and characterization of palm lethal decline phytoplasmas, subgroups 16SrIV-A and -D, in Phoenix canariensis and Syagrus romanzoffiana in Puebla, Mexico
title_full Detection and characterization of palm lethal decline phytoplasmas, subgroups 16SrIV-A and -D, in Phoenix canariensis and Syagrus romanzoffiana in Puebla, Mexico
title_fullStr Detection and characterization of palm lethal decline phytoplasmas, subgroups 16SrIV-A and -D, in Phoenix canariensis and Syagrus romanzoffiana in Puebla, Mexico
title_full_unstemmed Detection and characterization of palm lethal decline phytoplasmas, subgroups 16SrIV-A and -D, in Phoenix canariensis and Syagrus romanzoffiana in Puebla, Mexico
title_short Detection and characterization of palm lethal decline phytoplasmas, subgroups 16SrIV-A and -D, in Phoenix canariensis and Syagrus romanzoffiana in Puebla, Mexico
title_sort detection and characterization of palm lethal decline phytoplasmas subgroups 16sriv a and d in phoenix canariensis and syagrus romanzoffiana in puebla mexico
topic lethal bronzing
lethal yellowing
ornamental palms
phytoplasma diseases
texas phoenix palm decline
url https://journals.pan.pl/Content/134612/PDF/10_OA_JPPR_65_1_2035_Palmo-Cancina.pdf
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