Efficacy of Lacticaseibacillus rhamnosus probiotic strains in treating chromate induced dermatitis
Abstract Chromate induced dermatitis is a significant occupational health concern. Chromate (Cr) resistant Lacticaseibacillus rhamnosus strains were isolated from commercial probiotic sachets PREPRO and HiFLORA. Among 13 Cr-resistant bacterial isolates, six were selected based on high chromate resis...
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Nature Portfolio
2025-03-01
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| author | Mahnoor Ali Aatif Amin Zaigham Abbas |
| author_facet | Mahnoor Ali Aatif Amin Zaigham Abbas |
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| description | Abstract Chromate induced dermatitis is a significant occupational health concern. Chromate (Cr) resistant Lacticaseibacillus rhamnosus strains were isolated from commercial probiotic sachets PREPRO and HiFLORA. Among 13 Cr-resistant bacterial isolates, six were selected based on high chromate resistance at 500 ug/ml. Selected isolates were subjected to biochemical and molecular characterization and in vivo analyses. DPC assay was conducted under controlled conditions in order to determine the reduction potential of the isolated bacteria. The selected isolates were identified as L. rhamnosus-L1 (PP493917), L. rhamnosus-L2 (PP493918), L. rhamnosus-L3 (PP493921) L. rhamnosus-L4 (PP493920) L. rhamnosus-L8 (PP493922) and L. rhamnosus-L12 (PP493923). Lacticaseibacillus rhamnosus L1showed highest resistance against Cr (VI) with reduction potential 56%. In vivo experiments were performed to assess the healing effects of isolated bacterial strains on mice skin, with Hematoxylin and Eosin (H&E) staining used to identify severe dermatitis in skin tissue and evaluate therapeutic effects of probiotic strains. The structural determination of flavin reductase protein of L. rhamnosus was carried out using bioinformatics tools. These tools predicted the structural-based functional homology of flavin reductase protein in bacterial Cr (VI)-detoxification system. Lacticaseibacillus rhamnosus can be effectively used against chromate-induced dermatitis due to its high chromate resistance and reduction potential. |
| format | Article |
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| institution | DOAJ |
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| spelling | doaj-art-b794b41969d24dc8aeb6f84c251aa17f2025-08-20T03:01:38ZengNature PortfolioScientific Reports2045-23222025-03-0115111710.1038/s41598-025-93732-9Efficacy of Lacticaseibacillus rhamnosus probiotic strains in treating chromate induced dermatitisMahnoor Ali0Aatif Amin1Zaigham Abbas2Department of Microbiology, Faculty of Science and Technology, University of Central PunjabDepartment of Microbiology, Faculty of Science and Technology, University of Central PunjabInstitute of Microbiology and Molecular Genetics, Faculty of Life Sciences, University of the PunjabAbstract Chromate induced dermatitis is a significant occupational health concern. Chromate (Cr) resistant Lacticaseibacillus rhamnosus strains were isolated from commercial probiotic sachets PREPRO and HiFLORA. Among 13 Cr-resistant bacterial isolates, six were selected based on high chromate resistance at 500 ug/ml. Selected isolates were subjected to biochemical and molecular characterization and in vivo analyses. DPC assay was conducted under controlled conditions in order to determine the reduction potential of the isolated bacteria. The selected isolates were identified as L. rhamnosus-L1 (PP493917), L. rhamnosus-L2 (PP493918), L. rhamnosus-L3 (PP493921) L. rhamnosus-L4 (PP493920) L. rhamnosus-L8 (PP493922) and L. rhamnosus-L12 (PP493923). Lacticaseibacillus rhamnosus L1showed highest resistance against Cr (VI) with reduction potential 56%. In vivo experiments were performed to assess the healing effects of isolated bacterial strains on mice skin, with Hematoxylin and Eosin (H&E) staining used to identify severe dermatitis in skin tissue and evaluate therapeutic effects of probiotic strains. The structural determination of flavin reductase protein of L. rhamnosus was carried out using bioinformatics tools. These tools predicted the structural-based functional homology of flavin reductase protein in bacterial Cr (VI)-detoxification system. Lacticaseibacillus rhamnosus can be effectively used against chromate-induced dermatitis due to its high chromate resistance and reduction potential.https://doi.org/10.1038/s41598-025-93732-9Eradication treatmentChromate-associated inflammationLacticaseibacillus rhamnosusFlavin reductaseDermatitis animal studiesMolecular docking |
| spellingShingle | Mahnoor Ali Aatif Amin Zaigham Abbas Efficacy of Lacticaseibacillus rhamnosus probiotic strains in treating chromate induced dermatitis Scientific Reports Eradication treatment Chromate-associated inflammation Lacticaseibacillus rhamnosus Flavin reductase Dermatitis animal studies Molecular docking |
| title | Efficacy of Lacticaseibacillus rhamnosus probiotic strains in treating chromate induced dermatitis |
| title_full | Efficacy of Lacticaseibacillus rhamnosus probiotic strains in treating chromate induced dermatitis |
| title_fullStr | Efficacy of Lacticaseibacillus rhamnosus probiotic strains in treating chromate induced dermatitis |
| title_full_unstemmed | Efficacy of Lacticaseibacillus rhamnosus probiotic strains in treating chromate induced dermatitis |
| title_short | Efficacy of Lacticaseibacillus rhamnosus probiotic strains in treating chromate induced dermatitis |
| title_sort | efficacy of lacticaseibacillus rhamnosus probiotic strains in treating chromate induced dermatitis |
| topic | Eradication treatment Chromate-associated inflammation Lacticaseibacillus rhamnosus Flavin reductase Dermatitis animal studies Molecular docking |
| url | https://doi.org/10.1038/s41598-025-93732-9 |
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