Genome-wide identification of sucrose transporter genes in Camellia oleifera and characterization of CoSUT4
Sucrose transporters (SUTs) play a crucial role in carbon allocation from the source leaf to the sink end, and the function of SUTs varies among family members. However, the genome-wide identification of the SUT superfamily in Camellia oleifera is lacking, and their biological function remains elusi...
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KeAi Communications Co., Ltd.
2025-09-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311924002910 |
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| author | Jing Zhou Bingshuai Du Yibo Cao Kui Liu Zhihua Ye Yiming Huang Lingyun Zhang |
| author_facet | Jing Zhou Bingshuai Du Yibo Cao Kui Liu Zhihua Ye Yiming Huang Lingyun Zhang |
| author_sort | Jing Zhou |
| collection | DOAJ |
| description | Sucrose transporters (SUTs) play a crucial role in carbon allocation from the source leaf to the sink end, and the function of SUTs varies among family members. However, the genome-wide identification of the SUT superfamily in Camellia oleifera is lacking, and their biological function remains elusive. In this study, four SUT genes - designated CoSUT1-4 - were identified in C. oleifera through a genome-wide analysis and classified into three subfamilies. We used a combination of cis-acting elements analysis, mRNA quantification, histochemical analysis, and heterologous transformation to evaluate the expression profiles and functions of these SUTs. A key finding is that CoSUT4, localized on the plasma membrane, is highly expressed in mature leaves and the early stage of seed development in C. oleifera. In vitro culture of C. oleifera seed revealed the responsiveness of CoSUT4 to various exogenous hormones such as ABA and GA. CoSUT4 was able to restore the growth of the yeast strain SUSY7/ura3 (a sucrose transport-deficient mutant) on sucrose-containing media and specifically contributed to sucrose translocation and tissue growth in CoSUT4-overexpressed apple calli. In situ hybridization identified chalazal nucellus and transfer cells as the action sites of CoSUT4 at the maternal-filial interface mediating sucrose transportation in oil tea seeds. CoSUT4 overexpression in Arabidopsis thaliana atsuc4 mutant restored the growth and seed yield deficiencies of the mutant, leading to an increase in filled seeds and oil content. Additionally, CoSUT4 overexpression enhanced the drought and salt stress tolerance by augmenting sugar content. Overall, these findings provide valuable insights into the function of SUTs and present promising candidates for the genetic enhancement of seed production in C. oleifera. |
| format | Article |
| id | doaj-art-b77656b9f196403f80f796cbee1ec6c1 |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-b77656b9f196403f80f796cbee1ec6c12025-08-21T04:16:42ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192025-09-012493494351010.1016/j.jia.2024.07.048Genome-wide identification of sucrose transporter genes in Camellia oleifera and characterization of CoSUT4Jing Zhou0Bingshuai Du1Yibo Cao2Kui Liu3Zhihua Ye4Yiming Huang5Lingyun Zhang6State Key Laboratory of Efficient Production of Forest Resources/Key Laboratory of Forest Silviculture and Conservation of the Ministry of Education, College of Forestry, Beijing Forestry University, Beijing 100083, ChinaState Key Laboratory of Efficient Production of Forest Resources/Key Laboratory of Forest Silviculture and Conservation of the Ministry of Education, College of Forestry, Beijing Forestry University, Beijing 100083, ChinaState Key Laboratory of Efficient Production of Forest Resources/Key Laboratory of Forest Silviculture and Conservation of the Ministry of Education, College of Forestry, Beijing Forestry University, Beijing 100083, ChinaState Key Laboratory of Efficient Production of Forest Resources/Key Laboratory of Forest Silviculture and Conservation of the Ministry of Education, College of Forestry, Beijing Forestry University, Beijing 100083, ChinaState Key Laboratory of Efficient Production of Forest Resources/Key Laboratory of Forest Silviculture and Conservation of the Ministry of Education, College of Forestry, Beijing Forestry University, Beijing 100083, ChinaState Key Laboratory of Efficient Production of Forest Resources/Key Laboratory of Forest Silviculture and Conservation of the Ministry of Education, College of Forestry, Beijing Forestry University, Beijing 100083, ChinaCorrespondence Lingyun Zhang, Tel: +86-10-62336044, Fax: +86-10-62338197; State Key Laboratory of Efficient Production of Forest Resources/Key Laboratory of Forest Silviculture and Conservation of the Ministry of Education, College of Forestry, Beijing Forestry University, Beijing 100083, ChinaSucrose transporters (SUTs) play a crucial role in carbon allocation from the source leaf to the sink end, and the function of SUTs varies among family members. However, the genome-wide identification of the SUT superfamily in Camellia oleifera is lacking, and their biological function remains elusive. In this study, four SUT genes - designated CoSUT1-4 - were identified in C. oleifera through a genome-wide analysis and classified into three subfamilies. We used a combination of cis-acting elements analysis, mRNA quantification, histochemical analysis, and heterologous transformation to evaluate the expression profiles and functions of these SUTs. A key finding is that CoSUT4, localized on the plasma membrane, is highly expressed in mature leaves and the early stage of seed development in C. oleifera. In vitro culture of C. oleifera seed revealed the responsiveness of CoSUT4 to various exogenous hormones such as ABA and GA. CoSUT4 was able to restore the growth of the yeast strain SUSY7/ura3 (a sucrose transport-deficient mutant) on sucrose-containing media and specifically contributed to sucrose translocation and tissue growth in CoSUT4-overexpressed apple calli. In situ hybridization identified chalazal nucellus and transfer cells as the action sites of CoSUT4 at the maternal-filial interface mediating sucrose transportation in oil tea seeds. CoSUT4 overexpression in Arabidopsis thaliana atsuc4 mutant restored the growth and seed yield deficiencies of the mutant, leading to an increase in filled seeds and oil content. Additionally, CoSUT4 overexpression enhanced the drought and salt stress tolerance by augmenting sugar content. Overall, these findings provide valuable insights into the function of SUTs and present promising candidates for the genetic enhancement of seed production in C. oleifera.http://www.sciencedirect.com/science/article/pii/S2095311924002910Camellia oleiferasucrose transportergenome-wide analysissugar accumulationabiotic stress |
| spellingShingle | Jing Zhou Bingshuai Du Yibo Cao Kui Liu Zhihua Ye Yiming Huang Lingyun Zhang Genome-wide identification of sucrose transporter genes in Camellia oleifera and characterization of CoSUT4 Journal of Integrative Agriculture Camellia oleifera sucrose transporter genome-wide analysis sugar accumulation abiotic stress |
| title | Genome-wide identification of sucrose transporter genes in Camellia oleifera and characterization of CoSUT4 |
| title_full | Genome-wide identification of sucrose transporter genes in Camellia oleifera and characterization of CoSUT4 |
| title_fullStr | Genome-wide identification of sucrose transporter genes in Camellia oleifera and characterization of CoSUT4 |
| title_full_unstemmed | Genome-wide identification of sucrose transporter genes in Camellia oleifera and characterization of CoSUT4 |
| title_short | Genome-wide identification of sucrose transporter genes in Camellia oleifera and characterization of CoSUT4 |
| title_sort | genome wide identification of sucrose transporter genes in camellia oleifera and characterization of cosut4 |
| topic | Camellia oleifera sucrose transporter genome-wide analysis sugar accumulation abiotic stress |
| url | http://www.sciencedirect.com/science/article/pii/S2095311924002910 |
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