Role of MMP-2, MMP-9, TIMP-1, and TIMP-2 in children with ventricular septal defect
Ventricular septal defect (VSD) is the second most common congenital heart anomaly. In most cases, it closes spontaneously in the first year of life, but it sometimes requires surgical closure due to the risk of serious complications. This is why it is important to identify markers that could help...
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Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
2024-10-01
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| Series: | Biomolecules & Biomedicine |
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| Online Access: | https://www.bjbms.org/ojs/index.php/bjbms/article/view/11162 |
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| author | Nina Maric Ines Mrakovcic Sutic Jelica Predojevic Samardzic Dario Djukic Aleksandar Bulog Ivana Sutic |
| author_facet | Nina Maric Ines Mrakovcic Sutic Jelica Predojevic Samardzic Dario Djukic Aleksandar Bulog Ivana Sutic |
| author_sort | Nina Maric |
| collection | DOAJ |
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Ventricular septal defect (VSD) is the second most common congenital heart anomaly. In most cases, it closes spontaneously in the first year of life, but it sometimes requires surgical closure due to the risk of serious complications. This is why it is important to identify markers that could help predict its course. Findings that matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) play an important role in the cleavage of the extracellular matrix were the reasons to investigate their role in cardiogenesis. In prior studies on this topic, their concentrations were studied in the blood. The aim of this prospective study was to investigate the role of MMP-2, MMP-9, TIMP-1, and TIMP-2 in the etiology and pathophysiology of VSD using urine samples, as an innovative non-invasive approach, and the ELISA method. It involved fifty-two children with isolated VSD and twenty healthy children up to one year of age. We found that these MMPs and TIMPs are significantly (P = 0.000) higher in children with VSD, and the correlations between their concentrations and the size of the defect are positive, especially for MMP-9 and TIMP-1. MMP-9 was significantly (P = 0.044) higher in cases in which VSD didn't close in the first year of life compared to cases in which it closed. Our results suggest the role of MMP-2, MMP-9, TIMP-1, and TIMP-2 in the aetiopathogenesis of VSD and that their urinary concentrations, especially of MMP-9, in combination with echocardiographic and clinical monitoring, could be useful in predicting its natural course.
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| format | Article |
| id | doaj-art-b7706a1f8cac47498ea2d64b59f25295 |
| institution | OA Journals |
| issn | 2831-0896 2831-090X |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
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| series | Biomolecules & Biomedicine |
| spelling | doaj-art-b7706a1f8cac47498ea2d64b59f252952025-08-20T02:09:22ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2024-10-0110.17305/bb.2024.11162Role of MMP-2, MMP-9, TIMP-1, and TIMP-2 in children with ventricular septal defectNina Maric0https://orcid.org/0000-0003-3143-3098Ines Mrakovcic Sutic1Jelica Predojevic Samardzic2Dario Djukic3https://orcid.org/0000-0002-8017-0266Aleksandar Bulog4https://orcid.org/0000-0001-6283-9248Ivana Sutic5Clinic for Children's Diseases, University Clinical Centre of the Republic of Srpska, Banja Luka, Bosnia and Herzegovina; Department of Paediatrics, Medical Faculty, University of Banja Luka, Banja Luka, Bosnia and HerzegovinaDepartmant of Physiology, Immunology and Pathophysiology, Faculty of Medicine, University of Rijeka, Rijeka, CroatiaClinic for Children's Diseases, University Clinical Centre of the Republic of Srpska, Banja Luka, Bosnia and Herzegovina; Department of Paediatrics, Medical Faculty, University of Banja Luka, Banja Luka, Bosnia and HerzegovinaClinic for Children's Diseases, University Clinical Centre of the Republic of Srpska, Banja Luka, Bosnia and HerzegovinaDepartment of Health Ecology, Faculty of Medicine, University of Rijeka, Rijeka, Croatia; Institute of Public Health, Primorsko-Goranska County, Rijeka, CroatiaDepartment of Family Medicine, Medical Faculty, University of Rijeka, Rijeka, Croatia Ventricular septal defect (VSD) is the second most common congenital heart anomaly. In most cases, it closes spontaneously in the first year of life, but it sometimes requires surgical closure due to the risk of serious complications. This is why it is important to identify markers that could help predict its course. Findings that matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) play an important role in the cleavage of the extracellular matrix were the reasons to investigate their role in cardiogenesis. In prior studies on this topic, their concentrations were studied in the blood. The aim of this prospective study was to investigate the role of MMP-2, MMP-9, TIMP-1, and TIMP-2 in the etiology and pathophysiology of VSD using urine samples, as an innovative non-invasive approach, and the ELISA method. It involved fifty-two children with isolated VSD and twenty healthy children up to one year of age. We found that these MMPs and TIMPs are significantly (P = 0.000) higher in children with VSD, and the correlations between their concentrations and the size of the defect are positive, especially for MMP-9 and TIMP-1. MMP-9 was significantly (P = 0.044) higher in cases in which VSD didn't close in the first year of life compared to cases in which it closed. Our results suggest the role of MMP-2, MMP-9, TIMP-1, and TIMP-2 in the aetiopathogenesis of VSD and that their urinary concentrations, especially of MMP-9, in combination with echocardiographic and clinical monitoring, could be useful in predicting its natural course. https://www.bjbms.org/ojs/index.php/bjbms/article/view/11162Ventricular septal defectmatrix metalloproteinasestissue inhibitor of metalloproteinasesurine |
| spellingShingle | Nina Maric Ines Mrakovcic Sutic Jelica Predojevic Samardzic Dario Djukic Aleksandar Bulog Ivana Sutic Role of MMP-2, MMP-9, TIMP-1, and TIMP-2 in children with ventricular septal defect Biomolecules & Biomedicine Ventricular septal defect matrix metalloproteinases tissue inhibitor of metalloproteinases urine |
| title | Role of MMP-2, MMP-9, TIMP-1, and TIMP-2 in children with ventricular septal defect |
| title_full | Role of MMP-2, MMP-9, TIMP-1, and TIMP-2 in children with ventricular septal defect |
| title_fullStr | Role of MMP-2, MMP-9, TIMP-1, and TIMP-2 in children with ventricular septal defect |
| title_full_unstemmed | Role of MMP-2, MMP-9, TIMP-1, and TIMP-2 in children with ventricular septal defect |
| title_short | Role of MMP-2, MMP-9, TIMP-1, and TIMP-2 in children with ventricular septal defect |
| title_sort | role of mmp 2 mmp 9 timp 1 and timp 2 in children with ventricular septal defect |
| topic | Ventricular septal defect matrix metalloproteinases tissue inhibitor of metalloproteinases urine |
| url | https://www.bjbms.org/ojs/index.php/bjbms/article/view/11162 |
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