Enhancing total phenolic, flavonoid, and antioxidant capacity in black orchid (Coelogyne pandurata Lindl.) with paclobutrazol application

The Black Orchid (Coelogyne pandurata Lindl.) is a species with promising secondary metabolite content, including total phenolics, total flavonoids, and antioxidant capacity, making it highly valuable for horticultural applications. This research investigated the influence of Paclobutrazol applicati...

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Main Authors: Heriansyah Pebra, Aziz Sandra Arifin, Sukma Dewi, Nurcholis Waras
Format: Article
Language:English
Published: EDP Sciences 2025-01-01
Series:BIO Web of Conferences
Online Access:https://www.bio-conferences.org/articles/bioconf/pdf/2025/22/bioconf_fisaed2025_01007.pdf
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author Heriansyah Pebra
Aziz Sandra Arifin
Sukma Dewi
Nurcholis Waras
author_facet Heriansyah Pebra
Aziz Sandra Arifin
Sukma Dewi
Nurcholis Waras
author_sort Heriansyah Pebra
collection DOAJ
description The Black Orchid (Coelogyne pandurata Lindl.) is a species with promising secondary metabolite content, including total phenolics, total flavonoids, and antioxidant capacity, making it highly valuable for horticultural applications. This research investigated the influence of Paclobutrazol application on the total phenolic content, total flavonoid content, and antioxidant capacity of Black Orchid. The study was conducted using a randomized complete block design (RCBD) with Paclobutrazol treatments at concentrations of 0, 50, 100, and 150 ppm. Each experimental unit was conducted with three replicates. The data were analyzed using ANOVA, followed by Tukey’s HSD test at a 5% significance level to compare treatment means. Total phenolic, flavonoid, and antioxidant capacities were analysed using a Nano-spectrophotometer. Results indicated that treatment with 150 ppm Paclobutrazol significantly increased the flavonoid concentration in the leaves, showing a significant difference compared to the control. However, no significant effect was observed on the total phenolic content or antioxidant activity in either the leaves or bulbs.
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institution OA Journals
issn 2117-4458
language English
publishDate 2025-01-01
publisher EDP Sciences
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series BIO Web of Conferences
spelling doaj-art-b77064250e6c48bfa01797eeef2c61402025-08-20T02:09:34ZengEDP SciencesBIO Web of Conferences2117-44582025-01-011710100710.1051/bioconf/202517101007bioconf_fisaed2025_01007Enhancing total phenolic, flavonoid, and antioxidant capacity in black orchid (Coelogyne pandurata Lindl.) with paclobutrazol applicationHeriansyah Pebra0Aziz Sandra Arifin1Sukma Dewi2Nurcholis Waras3IPB University, Bogor/Department of Agronomy and Horticulture/Graduate SchoolDepartment of Agronomy and Horticulture, Faculty of Agriculture, IPB University.Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University.Department of Biochemistry, Faculty of Mathematics and Natural Sciences, IPB University.The Black Orchid (Coelogyne pandurata Lindl.) is a species with promising secondary metabolite content, including total phenolics, total flavonoids, and antioxidant capacity, making it highly valuable for horticultural applications. This research investigated the influence of Paclobutrazol application on the total phenolic content, total flavonoid content, and antioxidant capacity of Black Orchid. The study was conducted using a randomized complete block design (RCBD) with Paclobutrazol treatments at concentrations of 0, 50, 100, and 150 ppm. Each experimental unit was conducted with three replicates. The data were analyzed using ANOVA, followed by Tukey’s HSD test at a 5% significance level to compare treatment means. Total phenolic, flavonoid, and antioxidant capacities were analysed using a Nano-spectrophotometer. Results indicated that treatment with 150 ppm Paclobutrazol significantly increased the flavonoid concentration in the leaves, showing a significant difference compared to the control. However, no significant effect was observed on the total phenolic content or antioxidant activity in either the leaves or bulbs.https://www.bio-conferences.org/articles/bioconf/pdf/2025/22/bioconf_fisaed2025_01007.pdf
spellingShingle Heriansyah Pebra
Aziz Sandra Arifin
Sukma Dewi
Nurcholis Waras
Enhancing total phenolic, flavonoid, and antioxidant capacity in black orchid (Coelogyne pandurata Lindl.) with paclobutrazol application
BIO Web of Conferences
title Enhancing total phenolic, flavonoid, and antioxidant capacity in black orchid (Coelogyne pandurata Lindl.) with paclobutrazol application
title_full Enhancing total phenolic, flavonoid, and antioxidant capacity in black orchid (Coelogyne pandurata Lindl.) with paclobutrazol application
title_fullStr Enhancing total phenolic, flavonoid, and antioxidant capacity in black orchid (Coelogyne pandurata Lindl.) with paclobutrazol application
title_full_unstemmed Enhancing total phenolic, flavonoid, and antioxidant capacity in black orchid (Coelogyne pandurata Lindl.) with paclobutrazol application
title_short Enhancing total phenolic, flavonoid, and antioxidant capacity in black orchid (Coelogyne pandurata Lindl.) with paclobutrazol application
title_sort enhancing total phenolic flavonoid and antioxidant capacity in black orchid coelogyne pandurata lindl with paclobutrazol application
url https://www.bio-conferences.org/articles/bioconf/pdf/2025/22/bioconf_fisaed2025_01007.pdf
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