High Frequency Production of Doubled Haploid Rapeseed Plants by Direct Colchicine Treatment of Isolated Microspores
Techniques for microspore culture of Brassica napus have been improved rapidly, and embryogen-esis has been achieved in a wide range of genotypes. Plants regenerated from microspore-derived embryoids can be haploid, diploid or polyploid. From rapeseed microspore culture it is reported that 70%-90% o...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2000-03-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2000.02.0125 |
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| Summary: | Techniques for microspore culture of Brassica napus have been improved rapidly, and embryogen-esis has been achieved in a wide range of genotypes. Plants regenerated from microspore-derived embryoids can be haploid, diploid or polyploid. From rapeseed microspore culture it is reported that 70%-90% of regenerated plants are haploid. The usual methods of chromosome doubling involve soaking roots (most common) or whole plants in a colchicine solution, or culturing plantlets in colchicine-containing medium in the greenhouse. Other alternatives are injecting colchicine into the secondary buds or applying colchicine-soaked cotton plugs to axillary buds. |
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| ISSN: | 1008-9209 2097-5155 |