The Resistance to Freeze-Drying and to Storage Was Determined as the Cellular Ability to Recover Its Survival Rate and Acidification Activity
The protective effects of the fatty acid composition and membrane action of the acidification activity of two strains of Lactobacillus kept at 20∘C were studied. The addition of sorbitol, monosodium glutamate and glycerol during storage is causing the decline of acidification and increased concentra...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2010-01-01
|
| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2010/625239 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849395821305397248 |
|---|---|
| author | Ibourahema Coulibaly Robin Dubois-Dauphin Jacqueline Destain Marie-Laure Fauconnier Georges Lognay Philippe Thonart |
| author_facet | Ibourahema Coulibaly Robin Dubois-Dauphin Jacqueline Destain Marie-Laure Fauconnier Georges Lognay Philippe Thonart |
| author_sort | Ibourahema Coulibaly |
| collection | DOAJ |
| description | The protective effects of the fatty acid composition and membrane action of the acidification activity of two strains of Lactobacillus kept at 20∘C were studied. The addition of sorbitol, monosodium glutamate and glycerol during storage is causing the decline of acidification and increased concentrations of unsaturated fatty acids observed in both strains. The addition of sorbitol and monosodium glutamate does not alter the fatty acid composition, whatever the strain, but increases the resistance to freeze-drying of L. plantarum CWBI-B1419 and improves survival during storage. The addition of these preservatives and decreased activity of acidification improves the ratio unsaturated. These results indicate that the survival during storage and freeze-drying resistance are closely related to the composition of membrane fatty acids. This behaviour can be interpreted as an adaptation of L. plantarum B1419-CWBI supplemented by cryoprotectant additives such as sorbitol or monosodium glutamate sorbitol and monosodium glutamate as an additive. L. plantarum CWBI-B1419 presents a greater adaptation to culture conditions than L. paracasei ssp. paracasei LMG9192T. |
| format | Article |
| id | doaj-art-b76049ec95484d61b8ff673336367240 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-b76049ec95484d61b8ff6733363672402025-08-20T03:39:29ZengWileyInternational Journal of Microbiology1687-918X1687-91982010-01-01201010.1155/2010/625239625239The Resistance to Freeze-Drying and to Storage Was Determined as the Cellular Ability to Recover Its Survival Rate and Acidification ActivityIbourahema Coulibaly0Robin Dubois-Dauphin1Jacqueline Destain2Marie-Laure Fauconnier3Georges Lognay4Philippe Thonart5Wallon Center for Industrial Biology, Bio-Industry Unit, Gembloux Agricultural University, Passage des déportés 2, 5030 Gembloux, BelgiumWallon Center for Industrial Biology, Bio-Industry Unit, Gembloux Agricultural University, Passage des déportés 2, 5030 Gembloux, BelgiumWallon Center for Industrial Biology, Bio-Industry Unit, Gembloux Agricultural University, Passage des déportés 2, 5030 Gembloux, BelgiumAnalytical Chemistry Unit, Gembloux Agricultural University, Passages des déportés 2, 5030 Gembloux, BelgiumPlant Biology Unit, Gembloux Agricultural University, Passages des déportés 2, 5030 Gembloux, BelgiumWallon Center for Industrial Biology, Bio-Industry Unit, Gembloux Agricultural University, Passage des déportés 2, 5030 Gembloux, BelgiumThe protective effects of the fatty acid composition and membrane action of the acidification activity of two strains of Lactobacillus kept at 20∘C were studied. The addition of sorbitol, monosodium glutamate and glycerol during storage is causing the decline of acidification and increased concentrations of unsaturated fatty acids observed in both strains. The addition of sorbitol and monosodium glutamate does not alter the fatty acid composition, whatever the strain, but increases the resistance to freeze-drying of L. plantarum CWBI-B1419 and improves survival during storage. The addition of these preservatives and decreased activity of acidification improves the ratio unsaturated. These results indicate that the survival during storage and freeze-drying resistance are closely related to the composition of membrane fatty acids. This behaviour can be interpreted as an adaptation of L. plantarum B1419-CWBI supplemented by cryoprotectant additives such as sorbitol or monosodium glutamate sorbitol and monosodium glutamate as an additive. L. plantarum CWBI-B1419 presents a greater adaptation to culture conditions than L. paracasei ssp. paracasei LMG9192T.http://dx.doi.org/10.1155/2010/625239 |
| spellingShingle | Ibourahema Coulibaly Robin Dubois-Dauphin Jacqueline Destain Marie-Laure Fauconnier Georges Lognay Philippe Thonart The Resistance to Freeze-Drying and to Storage Was Determined as the Cellular Ability to Recover Its Survival Rate and Acidification Activity International Journal of Microbiology |
| title | The Resistance to Freeze-Drying and to Storage Was Determined as the Cellular Ability to Recover Its Survival Rate and Acidification Activity |
| title_full | The Resistance to Freeze-Drying and to Storage Was Determined as the Cellular Ability to Recover Its Survival Rate and Acidification Activity |
| title_fullStr | The Resistance to Freeze-Drying and to Storage Was Determined as the Cellular Ability to Recover Its Survival Rate and Acidification Activity |
| title_full_unstemmed | The Resistance to Freeze-Drying and to Storage Was Determined as the Cellular Ability to Recover Its Survival Rate and Acidification Activity |
| title_short | The Resistance to Freeze-Drying and to Storage Was Determined as the Cellular Ability to Recover Its Survival Rate and Acidification Activity |
| title_sort | resistance to freeze drying and to storage was determined as the cellular ability to recover its survival rate and acidification activity |
| url | http://dx.doi.org/10.1155/2010/625239 |
| work_keys_str_mv | AT ibourahemacoulibaly theresistancetofreezedryingandtostoragewasdeterminedasthecellularabilitytorecoveritssurvivalrateandacidificationactivity AT robinduboisdauphin theresistancetofreezedryingandtostoragewasdeterminedasthecellularabilitytorecoveritssurvivalrateandacidificationactivity AT jacquelinedestain theresistancetofreezedryingandtostoragewasdeterminedasthecellularabilitytorecoveritssurvivalrateandacidificationactivity AT marielaurefauconnier theresistancetofreezedryingandtostoragewasdeterminedasthecellularabilitytorecoveritssurvivalrateandacidificationactivity AT georgeslognay theresistancetofreezedryingandtostoragewasdeterminedasthecellularabilitytorecoveritssurvivalrateandacidificationactivity AT philippethonart theresistancetofreezedryingandtostoragewasdeterminedasthecellularabilitytorecoveritssurvivalrateandacidificationactivity AT ibourahemacoulibaly resistancetofreezedryingandtostoragewasdeterminedasthecellularabilitytorecoveritssurvivalrateandacidificationactivity AT robinduboisdauphin resistancetofreezedryingandtostoragewasdeterminedasthecellularabilitytorecoveritssurvivalrateandacidificationactivity AT jacquelinedestain resistancetofreezedryingandtostoragewasdeterminedasthecellularabilitytorecoveritssurvivalrateandacidificationactivity AT marielaurefauconnier resistancetofreezedryingandtostoragewasdeterminedasthecellularabilitytorecoveritssurvivalrateandacidificationactivity AT georgeslognay resistancetofreezedryingandtostoragewasdeterminedasthecellularabilitytorecoveritssurvivalrateandacidificationactivity AT philippethonart resistancetofreezedryingandtostoragewasdeterminedasthecellularabilitytorecoveritssurvivalrateandacidificationactivity |