Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus isolates in Eastern China
Introduction: Porcine epidemic diarrhea virus (PEDV) is one of the most common viral pathogens causing swine diarrhea. Methodology: We performed a genetic evolution analysis of the S1 gene of endemic PEDV strains in Eastern China. The S1 genes of 37 PEDV-positive samples were amplified and seque...
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The Journal of Infection in Developing Countries
2018-12-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/9898 |
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| author | Chunyan Jiang Xiaoju Zhang Jianfeng Han Haijian He Chaoying Zhang Hongbing Zhang Jingjing Jin Liang Wang Bingqian Ge Yanli Wang Yongjie Liu |
| author_facet | Chunyan Jiang Xiaoju Zhang Jianfeng Han Haijian He Chaoying Zhang Hongbing Zhang Jingjing Jin Liang Wang Bingqian Ge Yanli Wang Yongjie Liu |
| author_sort | Chunyan Jiang |
| collection | DOAJ |
| description |
Introduction: Porcine epidemic diarrhea virus (PEDV) is one of the most common viral pathogens causing swine diarrhea.
Methodology: We performed a genetic evolution analysis of the S1 gene of endemic PEDV strains in Eastern China. The S1 genes of 37 PEDV-positive samples were amplified and sequenced, and compared to the standard CV777 strain, 120 nucleotides were found to have mutations.
Results: The nucleotide and deduced amino acid homologies between the sequences and those of the CV777 strain were 90%–91% and 88.2%–90%, respectively, and their homologies to the vaccine strain were 88.6%–89.7% and 86.2%–87.8%, respectively. Genetic evolution and variation analyses indicated that the 37 PEDV strains belonged to genogroup 2-1, while the CV777 strain, vaccine strain, and earlier Chinese strains all belonged to genogroup 1-1.
Conclusions: The newly emerged clinical PEDV strains indicate that the PEDV CV777 vaccine currently used in China may not fully protect pigs from infection with recent epidemic strains, and will require the development of new vaccine strains.
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| format | Article |
| id | doaj-art-b7543cf5fe294a3598b109c40eb030f1 |
| institution | DOAJ |
| issn | 1972-2680 |
| language | English |
| publishDate | 2018-12-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-b7543cf5fe294a3598b109c40eb030f12025-08-20T02:57:13ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802018-12-01121210.3855/jidc.9898Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus isolates in Eastern ChinaChunyan Jiang0Xiaoju Zhang1Jianfeng Han2Haijian He3Chaoying Zhang4Hongbing Zhang5Jingjing Jin6Liang Wang7Bingqian Ge8Yanli Wang9Yongjie Liu10Jinhua Polytechnic, Jinhua, Zhejiang, ChinaJinhua Polytechnic, Jinhua, Zhejiang, ChinaChinese Academy of Agricultural Sciences, Beijing, ChinaJinhua Polytechnic, Jinhua, Zhejiang, ChinaJinhua Polytechnic, Jinhua, Zhejiang, ChinaJinhua Polytechnic, Jinhua, Zhejiang, ChinaJinhua Polytechnic, Jinhua, Zhejiang, ChinaShanxi Yuncheng Agricultural Vocational And Technical College, Yuncheng, Shanxi, ChinaJinhua Polytechnic, Jinhua, Zhejiang, ChinaJinhua Polytechnic, Jinhua, Zhejiang, ChinaNanjing Agricultural University, Nanjing, Jiangsu, China Introduction: Porcine epidemic diarrhea virus (PEDV) is one of the most common viral pathogens causing swine diarrhea. Methodology: We performed a genetic evolution analysis of the S1 gene of endemic PEDV strains in Eastern China. The S1 genes of 37 PEDV-positive samples were amplified and sequenced, and compared to the standard CV777 strain, 120 nucleotides were found to have mutations. Results: The nucleotide and deduced amino acid homologies between the sequences and those of the CV777 strain were 90%–91% and 88.2%–90%, respectively, and their homologies to the vaccine strain were 88.6%–89.7% and 86.2%–87.8%, respectively. Genetic evolution and variation analyses indicated that the 37 PEDV strains belonged to genogroup 2-1, while the CV777 strain, vaccine strain, and earlier Chinese strains all belonged to genogroup 1-1. Conclusions: The newly emerged clinical PEDV strains indicate that the PEDV CV777 vaccine currently used in China may not fully protect pigs from infection with recent epidemic strains, and will require the development of new vaccine strains. https://jidc.org/index.php/journal/article/view/9898Swine diarrheaporcine epidemic diarrhea virusS1 genegenetic evolution analysis |
| spellingShingle | Chunyan Jiang Xiaoju Zhang Jianfeng Han Haijian He Chaoying Zhang Hongbing Zhang Jingjing Jin Liang Wang Bingqian Ge Yanli Wang Yongjie Liu Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus isolates in Eastern China Journal of Infection in Developing Countries Swine diarrhea porcine epidemic diarrhea virus S1 gene genetic evolution analysis |
| title | Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus isolates in Eastern China |
| title_full | Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus isolates in Eastern China |
| title_fullStr | Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus isolates in Eastern China |
| title_full_unstemmed | Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus isolates in Eastern China |
| title_short | Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus isolates in Eastern China |
| title_sort | molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus isolates in eastern china |
| topic | Swine diarrhea porcine epidemic diarrhea virus S1 gene genetic evolution analysis |
| url | https://jidc.org/index.php/journal/article/view/9898 |
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