Histomorphological scoring of murine colitis models: A practical guide for the evaluation of colitis and colitis-associated cancer
Background and aims: Histomorphology is a powerful and cost-efficient tool for evaluating inflammatory and neoplastic conditions. Inflammatory bowel disease (IBD) is a widespread condition with globally rising incidences, and a lot of research is done to better understand the pathogenesis of IBD and...
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Elsevier
2024-12-01
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| Series: | Experimental and Molecular Pathology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0014480024000571 |
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| author | Marianne Remke Tanja Groll Thomas Metzler Elisabeth Urbauer Janine Kövilein Theresa Schnalzger Jürgen Ruland Dirk Haller Katja Steiger |
| author_facet | Marianne Remke Tanja Groll Thomas Metzler Elisabeth Urbauer Janine Kövilein Theresa Schnalzger Jürgen Ruland Dirk Haller Katja Steiger |
| author_sort | Marianne Remke |
| collection | DOAJ |
| description | Background and aims: Histomorphology is a powerful and cost-efficient tool for evaluating inflammatory and neoplastic conditions. Inflammatory bowel disease (IBD) is a widespread condition with globally rising incidences, and a lot of research is done to better understand the pathogenesis of IBD and to identify potential therapeutic approaches. However, standardized and reproducible scores for the histomorphological evaluation of murine IBD models are lacking. Therefore, we aimed to develop an easy-to-use and reproducible score for standardized assessment of colitis and associated cancer models. Methods: In this study, samples from three different colitis models with and without associated cancer formation were analyzed to develop a universal, robust, and reproducible score for the grading of murine colitis models using the following three parameters: 1. Extent of leucocyte infiltration, 2. Tissue damage, 3. Architectural disruption of the mucosa. Results: A scoring system was established for different kinds of colitis models (genetically induced enterocolitis, genetically induced metabolic injury, and chemically induced colitis-associated cancer) and all stages of the disease, from mild inflammatory changes to severe inflammation with neoplastic changes as the extreme extent of IBD. The scoring scheme is easy to use, can easily be learned, and proves to have a high interrater reliability. Conclusions: We propose a robust histological scoring system for the assessment of murine colitis and colitis-associated cancer models, giving more researchers access to conclusive and reliable histological assessment. |
| format | Article |
| id | doaj-art-b750da9b764f4fdf80f6f9c0ac095865 |
| institution | OA Journals |
| issn | 1096-0945 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Experimental and Molecular Pathology |
| spelling | doaj-art-b750da9b764f4fdf80f6f9c0ac0958652025-08-20T01:57:55ZengElsevierExperimental and Molecular Pathology1096-09452024-12-0114010493810.1016/j.yexmp.2024.104938Histomorphological scoring of murine colitis models: A practical guide for the evaluation of colitis and colitis-associated cancerMarianne Remke0Tanja Groll1Thomas Metzler2Elisabeth Urbauer3Janine Kövilein4Theresa Schnalzger5Jürgen Ruland6Dirk Haller7Katja Steiger8Institute of Pathology, School of Medicine and Health, Technical University of Munich, Trogerstr. 18, 81675 Munich, Germany; Comparative Experimental Pathology, School of Medicine and Health, Technical University of Munich, Trogerstr. 18, 81675 Munich, Germany; Member of the German Cancer Consortium (DKTK), Partner Site Munich, Munich, Germany; Corresponding author at: Institut für Pathologie TU München, Trogerstraße 18, 81675 München, Germany.Institute of Pathology, School of Medicine and Health, Technical University of Munich, Trogerstr. 18, 81675 Munich, Germany; Comparative Experimental Pathology, School of Medicine and Health, Technical University of Munich, Trogerstr. 18, 81675 Munich, GermanyInstitute of Pathology, School of Medicine and Health, Technical University of Munich, Trogerstr. 18, 81675 Munich, Germany; Comparative Experimental Pathology, School of Medicine and Health, Technical University of Munich, Trogerstr. 18, 81675 Munich, GermanyChair of Nutrition and Immunology, Technical University of Munich, Gregor-Mendel-Str. 2, 85354 Freising, GermanyChair of Nutrition and Immunology, Technical University of Munich, Gregor-Mendel-Str. 2, 85354 Freising, GermanyInstitute of Clinical Chemistry and Pathobiochemistry, School of Medicine and Health, Technical University of Munich, Ismaninger Str. 22, 81675 Munich, GermanyInstitute of Clinical Chemistry and Pathobiochemistry, School of Medicine and Health, Technical University of Munich, Ismaninger Str. 22, 81675 Munich, GermanyChair of Nutrition and Immunology, Technical University of Munich, Gregor-Mendel-Str. 2, 85354 Freising, GermanyInstitute of Pathology, School of Medicine and Health, Technical University of Munich, Trogerstr. 18, 81675 Munich, Germany; Comparative Experimental Pathology, School of Medicine and Health, Technical University of Munich, Trogerstr. 18, 81675 Munich, Germany; Member of the German Cancer Consortium (DKTK), Partner Site Munich, Munich, GermanyBackground and aims: Histomorphology is a powerful and cost-efficient tool for evaluating inflammatory and neoplastic conditions. Inflammatory bowel disease (IBD) is a widespread condition with globally rising incidences, and a lot of research is done to better understand the pathogenesis of IBD and to identify potential therapeutic approaches. However, standardized and reproducible scores for the histomorphological evaluation of murine IBD models are lacking. Therefore, we aimed to develop an easy-to-use and reproducible score for standardized assessment of colitis and associated cancer models. Methods: In this study, samples from three different colitis models with and without associated cancer formation were analyzed to develop a universal, robust, and reproducible score for the grading of murine colitis models using the following three parameters: 1. Extent of leucocyte infiltration, 2. Tissue damage, 3. Architectural disruption of the mucosa. Results: A scoring system was established for different kinds of colitis models (genetically induced enterocolitis, genetically induced metabolic injury, and chemically induced colitis-associated cancer) and all stages of the disease, from mild inflammatory changes to severe inflammation with neoplastic changes as the extreme extent of IBD. The scoring scheme is easy to use, can easily be learned, and proves to have a high interrater reliability. Conclusions: We propose a robust histological scoring system for the assessment of murine colitis and colitis-associated cancer models, giving more researchers access to conclusive and reliable histological assessment.http://www.sciencedirect.com/science/article/pii/S0014480024000571Experimental pathologyInflammatory bowel diseasesInflammationAnimal models |
| spellingShingle | Marianne Remke Tanja Groll Thomas Metzler Elisabeth Urbauer Janine Kövilein Theresa Schnalzger Jürgen Ruland Dirk Haller Katja Steiger Histomorphological scoring of murine colitis models: A practical guide for the evaluation of colitis and colitis-associated cancer Experimental and Molecular Pathology Experimental pathology Inflammatory bowel diseases Inflammation Animal models |
| title | Histomorphological scoring of murine colitis models: A practical guide for the evaluation of colitis and colitis-associated cancer |
| title_full | Histomorphological scoring of murine colitis models: A practical guide for the evaluation of colitis and colitis-associated cancer |
| title_fullStr | Histomorphological scoring of murine colitis models: A practical guide for the evaluation of colitis and colitis-associated cancer |
| title_full_unstemmed | Histomorphological scoring of murine colitis models: A practical guide for the evaluation of colitis and colitis-associated cancer |
| title_short | Histomorphological scoring of murine colitis models: A practical guide for the evaluation of colitis and colitis-associated cancer |
| title_sort | histomorphological scoring of murine colitis models a practical guide for the evaluation of colitis and colitis associated cancer |
| topic | Experimental pathology Inflammatory bowel diseases Inflammation Animal models |
| url | http://www.sciencedirect.com/science/article/pii/S0014480024000571 |
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