Physical Properties of Lens Membranes in Animals with Different Lifespans
The lipid composition of eye lens fiber cell membranes varies among species, and these differences increase as a function of lifespan. However, the way in which the lipids of the fiber cell membranes are organized in different animals has not yet been studied in detail. This study compares how the s...
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MDPI AG
2025-06-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/15/6/851 |
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| author | Marija Raguz Witold Karol Subczynski |
| author_facet | Marija Raguz Witold Karol Subczynski |
| author_sort | Marija Raguz |
| collection | DOAJ |
| description | The lipid composition of eye lens fiber cell membranes varies among species, and these differences increase as a function of lifespan. However, the way in which the lipids of the fiber cell membranes are organized in different animals has not yet been studied in detail. This study compares how the structure, properties, and organization of lipids change during the lifespan of the mouse, pig, and human. These species were chosen because of the wide range of lifespans and significant differences in lipid composition. Models were made of phospholipid compositions resembling those of the lens fiber cell membranes of a mouse, pig, and human aged of 3, 23, and 70 years, respectively. To separate the effects of phospholipids and cholesterol, membranes were investigated for samples without cholesterol, with a cholesterol content close to the cholesterol saturation limits, and with a cholesterol content close to the cholesterol solubility thresholds. Membrane physical properties were obtained using EPR spin labeling methods. No significant differences in the physical properties of the membranes of any of the models were detected for membranes without and those in the presence of a saturating cholesterol concentration. Thus, not only cholesterol but also the appropriate phospholipid composition is a significant factor in maintaining eye lens homeostasis. |
| format | Article |
| id | doaj-art-b75007f96bf54bbab2eef46bdd159796 |
| institution | Kabale University |
| issn | 2218-273X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj-art-b75007f96bf54bbab2eef46bdd1597962025-08-20T03:26:25ZengMDPI AGBiomolecules2218-273X2025-06-0115685110.3390/biom15060851Physical Properties of Lens Membranes in Animals with Different LifespansMarija Raguz0Witold Karol Subczynski1Department of Medical Physics and Biophysics, University of Split School of Medicine, 21000 Split, CroatiaDepartment of Biophysics, Medical College on Wisconsin, Milwaukee, WI 53226, USAThe lipid composition of eye lens fiber cell membranes varies among species, and these differences increase as a function of lifespan. However, the way in which the lipids of the fiber cell membranes are organized in different animals has not yet been studied in detail. This study compares how the structure, properties, and organization of lipids change during the lifespan of the mouse, pig, and human. These species were chosen because of the wide range of lifespans and significant differences in lipid composition. Models were made of phospholipid compositions resembling those of the lens fiber cell membranes of a mouse, pig, and human aged of 3, 23, and 70 years, respectively. To separate the effects of phospholipids and cholesterol, membranes were investigated for samples without cholesterol, with a cholesterol content close to the cholesterol saturation limits, and with a cholesterol content close to the cholesterol solubility thresholds. Membrane physical properties were obtained using EPR spin labeling methods. No significant differences in the physical properties of the membranes of any of the models were detected for membranes without and those in the presence of a saturating cholesterol concentration. Thus, not only cholesterol but also the appropriate phospholipid composition is a significant factor in maintaining eye lens homeostasis.https://www.mdpi.com/2218-273X/15/6/851eye lensphospholipidscholesterolprotection mechanismslifespan |
| spellingShingle | Marija Raguz Witold Karol Subczynski Physical Properties of Lens Membranes in Animals with Different Lifespans Biomolecules eye lens phospholipids cholesterol protection mechanisms lifespan |
| title | Physical Properties of Lens Membranes in Animals with Different Lifespans |
| title_full | Physical Properties of Lens Membranes in Animals with Different Lifespans |
| title_fullStr | Physical Properties of Lens Membranes in Animals with Different Lifespans |
| title_full_unstemmed | Physical Properties of Lens Membranes in Animals with Different Lifespans |
| title_short | Physical Properties of Lens Membranes in Animals with Different Lifespans |
| title_sort | physical properties of lens membranes in animals with different lifespans |
| topic | eye lens phospholipids cholesterol protection mechanisms lifespan |
| url | https://www.mdpi.com/2218-273X/15/6/851 |
| work_keys_str_mv | AT marijaraguz physicalpropertiesoflensmembranesinanimalswithdifferentlifespans AT witoldkarolsubczynski physicalpropertiesoflensmembranesinanimalswithdifferentlifespans |