Effect of nanoemulsion carbonated hydroxyapatite-statin administration on Acp 5 and Runx-2 expression during orthodontic relapse in rats
Background: Retention is an essential element of orthodontic treatment. In the past two decades, numerous biological treatments have been developed to alleviate orthodontic relapse. Pharmacologic bone modulation is a viable approach to mitigate relapse. The aim of this investigation was to determine...
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Elsevier
2025-01-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212426824001891 |
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| author | Ananto Ali Alhasyimi Niswati Fathmah Rosyida Ika Dewi Ana |
| author_facet | Ananto Ali Alhasyimi Niswati Fathmah Rosyida Ika Dewi Ana |
| author_sort | Ananto Ali Alhasyimi |
| collection | DOAJ |
| description | Background: Retention is an essential element of orthodontic treatment. In the past two decades, numerous biological treatments have been developed to alleviate orthodontic relapse. Pharmacologic bone modulation is a viable approach to mitigate relapse. The aim of this investigation was to determine whether administering nanoemulsion carbonated hydroxyapatite-statin (CHA-statin) would increase Runx-2 expression and decrease Acp 5 expression in rats experiencing experimental orthodontic relapse. Materials & methods: Forty-eight rats (n = 48) were assigned to four groups: control, CHA, statin, and CHA-statin, with 12 rats in each group. A 30 g mesial traction was applied for 7 days via a closed-coil spring that connected the first maxillary molar to the maxillary incisor. To maintain the moved teeth, CHA, statin hydrogel, and nanoemulsion CHA-statin were administered intrasulcularly every 3 days for a period of 7 days. The removal of the devices facilitated the occurrence of relapse. The expression of Acp 5 and Runx-2 was evaluated using the immunohistochemistry method. The collected data were evaluated using analysis of variance and post hoc tests, with p < 0.05. Results: A significant reduction in Acp 5-positive cells in the CHA-statin group was observed on days 7 and 14 of relapse movement compared with the other groups (p < 0.05), whereas the average Runx-2 expressions in the CHA-statin groups were significantly higher than in the other groups on days 1, 7, and 14 during the relapse phase (p < 0.05). Conclusion: The nanoemulsion CHA-statin increased Runx-2 expression and decreased Acp 5 expression, thereby potentially preventing orthodontic relapse in rats. |
| format | Article |
| id | doaj-art-b7464b9cb9b248bb98fda5e34ab6a697 |
| institution | Kabale University |
| issn | 2212-4268 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-b7464b9cb9b248bb98fda5e34ab6a6972025-02-09T05:00:13ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682025-01-01151129135Effect of nanoemulsion carbonated hydroxyapatite-statin administration on Acp 5 and Runx-2 expression during orthodontic relapse in ratsAnanto Ali Alhasyimi0Niswati Fathmah Rosyida1Ika Dewi Ana2Department of Orthodontics, Faculty of Dentistry, Universitas Gadjah Mada, Indonesia; Corresponding author. Department of Orthodontics, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia.Department of Orthodontics, Faculty of Dentistry, Universitas Gadjah Mada, IndonesiaDepartment of Dental Biomedical Sciences, Faculty of Dentistry, Universitas Gadjah Mada, Indonesia; Research Collaboration Center for Biomedical Scaffolds, National Research and Innovation Agency (BRIN) and Universitas Gadjah Mada (UGM), IndonesiaBackground: Retention is an essential element of orthodontic treatment. In the past two decades, numerous biological treatments have been developed to alleviate orthodontic relapse. Pharmacologic bone modulation is a viable approach to mitigate relapse. The aim of this investigation was to determine whether administering nanoemulsion carbonated hydroxyapatite-statin (CHA-statin) would increase Runx-2 expression and decrease Acp 5 expression in rats experiencing experimental orthodontic relapse. Materials & methods: Forty-eight rats (n = 48) were assigned to four groups: control, CHA, statin, and CHA-statin, with 12 rats in each group. A 30 g mesial traction was applied for 7 days via a closed-coil spring that connected the first maxillary molar to the maxillary incisor. To maintain the moved teeth, CHA, statin hydrogel, and nanoemulsion CHA-statin were administered intrasulcularly every 3 days for a period of 7 days. The removal of the devices facilitated the occurrence of relapse. The expression of Acp 5 and Runx-2 was evaluated using the immunohistochemistry method. The collected data were evaluated using analysis of variance and post hoc tests, with p < 0.05. Results: A significant reduction in Acp 5-positive cells in the CHA-statin group was observed on days 7 and 14 of relapse movement compared with the other groups (p < 0.05), whereas the average Runx-2 expressions in the CHA-statin groups were significantly higher than in the other groups on days 1, 7, and 14 during the relapse phase (p < 0.05). Conclusion: The nanoemulsion CHA-statin increased Runx-2 expression and decreased Acp 5 expression, thereby potentially preventing orthodontic relapse in rats.http://www.sciencedirect.com/science/article/pii/S2212426824001891Orthodontic retainerRelapseSimvastatinHydroxyapatitesNanoemulsion |
| spellingShingle | Ananto Ali Alhasyimi Niswati Fathmah Rosyida Ika Dewi Ana Effect of nanoemulsion carbonated hydroxyapatite-statin administration on Acp 5 and Runx-2 expression during orthodontic relapse in rats Journal of Oral Biology and Craniofacial Research Orthodontic retainer Relapse Simvastatin Hydroxyapatites Nanoemulsion |
| title | Effect of nanoemulsion carbonated hydroxyapatite-statin administration on Acp 5 and Runx-2 expression during orthodontic relapse in rats |
| title_full | Effect of nanoemulsion carbonated hydroxyapatite-statin administration on Acp 5 and Runx-2 expression during orthodontic relapse in rats |
| title_fullStr | Effect of nanoemulsion carbonated hydroxyapatite-statin administration on Acp 5 and Runx-2 expression during orthodontic relapse in rats |
| title_full_unstemmed | Effect of nanoemulsion carbonated hydroxyapatite-statin administration on Acp 5 and Runx-2 expression during orthodontic relapse in rats |
| title_short | Effect of nanoemulsion carbonated hydroxyapatite-statin administration on Acp 5 and Runx-2 expression during orthodontic relapse in rats |
| title_sort | effect of nanoemulsion carbonated hydroxyapatite statin administration on acp 5 and runx 2 expression during orthodontic relapse in rats |
| topic | Orthodontic retainer Relapse Simvastatin Hydroxyapatites Nanoemulsion |
| url | http://www.sciencedirect.com/science/article/pii/S2212426824001891 |
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