Use of real-time polymerase chain reaction to identify Entamoeba histolytica in schoolchildren from northwest Mexico
Introduction: Entamoeba histolytica, E. dispar, and E. moshkovskii are morphologically identical, but intestinal amebiasis is caused only by E. histolytica. Mexico is among the countries with high amebae infection rates, although the contribution of pathogenic amoeba to the total detected cases rem...
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The Journal of Infection in Developing Countries
2017-10-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/9350 |
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| author | Sandra Aguayo-Patrón Reyna Castillo-Fimbres Luis Quihui-Cota Ana María Calderón de la Barca |
| author_facet | Sandra Aguayo-Patrón Reyna Castillo-Fimbres Luis Quihui-Cota Ana María Calderón de la Barca |
| author_sort | Sandra Aguayo-Patrón |
| collection | DOAJ |
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Introduction: Entamoeba histolytica, E. dispar, and E. moshkovskii are morphologically identical, but intestinal amebiasis is caused only by E. histolytica. Mexico is among the countries with high amebae infection rates, although the contribution of pathogenic amoeba to the total detected cases remains unknown, especially in the northwestern dry region. Therefore, the aim of this study was to identify the actual prevalence of E. histolytica using real-time polymerase chain reaction (PCR) in schoolchildren of northwestern Mexico.
Methodology: Participants were children from five public elementary schools in the low-socioeconomic-level suburban areas of Hermosillo, Sonora, Mexico. One stool sample was collected from each child and analyzed by the Faust technique for Entamoeba spp. and by real-time PCR for E. histolytica.
Results: Analysis of stool samples from 273 children (9.0 ± 1.5 years of age) resulted in 25 (9.2%) positive for E. histolytica/E. dispar/E. moshkovskii by the Faust technique; of these, 3 were positive for E. histolytica by real-time PCR. In addition, 2 samples that were negative for E. histolytica/E. dispar/E. moshkovskii by the Faust technique were positive by real-time PCR.
Conclusions: The actual prevalence of E. histolytica in our study population was 1.8%, which is lower than those reported in previous studies in other Mexican regions.
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| format | Article |
| id | doaj-art-b73a63648a974fec9d53d561fddbfa98 |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2017-10-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-b73a63648a974fec9d53d561fddbfa982025-08-20T03:52:43ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802017-10-01111010.3855/jidc.9350Use of real-time polymerase chain reaction to identify Entamoeba histolytica in schoolchildren from northwest MexicoSandra Aguayo-Patrón0Reyna Castillo-Fimbres1Luis Quihui-Cota2Ana María Calderón de la Barca3Centro de Investigación en Alimentación y Desarrollo A. C., Hermosillo, Sonora, MéxicoCentro de Investigación en Alimentación y Desarrollo A. C., Hermosillo, Sonora, MéxicoCentro de Investigación en Alimentación y Desarrollo A. C., Hermosillo, Sonora, MéxicoCentro de Investigación en Alimentación y Desarrollo A. C., Hermosillo, Sonora, México Introduction: Entamoeba histolytica, E. dispar, and E. moshkovskii are morphologically identical, but intestinal amebiasis is caused only by E. histolytica. Mexico is among the countries with high amebae infection rates, although the contribution of pathogenic amoeba to the total detected cases remains unknown, especially in the northwestern dry region. Therefore, the aim of this study was to identify the actual prevalence of E. histolytica using real-time polymerase chain reaction (PCR) in schoolchildren of northwestern Mexico. Methodology: Participants were children from five public elementary schools in the low-socioeconomic-level suburban areas of Hermosillo, Sonora, Mexico. One stool sample was collected from each child and analyzed by the Faust technique for Entamoeba spp. and by real-time PCR for E. histolytica. Results: Analysis of stool samples from 273 children (9.0 ± 1.5 years of age) resulted in 25 (9.2%) positive for E. histolytica/E. dispar/E. moshkovskii by the Faust technique; of these, 3 were positive for E. histolytica by real-time PCR. In addition, 2 samples that were negative for E. histolytica/E. dispar/E. moshkovskii by the Faust technique were positive by real-time PCR. Conclusions: The actual prevalence of E. histolytica in our study population was 1.8%, which is lower than those reported in previous studies in other Mexican regions. https://jidc.org/index.php/journal/article/view/9350Entamoeba histolyticareal-time PCRreal time PCRschool childrenschoolchildrenidentification |
| spellingShingle | Sandra Aguayo-Patrón Reyna Castillo-Fimbres Luis Quihui-Cota Ana María Calderón de la Barca Use of real-time polymerase chain reaction to identify Entamoeba histolytica in schoolchildren from northwest Mexico Journal of Infection in Developing Countries Entamoeba histolytica real-time PCR real time PCR school children schoolchildren identification |
| title | Use of real-time polymerase chain reaction to identify Entamoeba histolytica in schoolchildren from northwest Mexico |
| title_full | Use of real-time polymerase chain reaction to identify Entamoeba histolytica in schoolchildren from northwest Mexico |
| title_fullStr | Use of real-time polymerase chain reaction to identify Entamoeba histolytica in schoolchildren from northwest Mexico |
| title_full_unstemmed | Use of real-time polymerase chain reaction to identify Entamoeba histolytica in schoolchildren from northwest Mexico |
| title_short | Use of real-time polymerase chain reaction to identify Entamoeba histolytica in schoolchildren from northwest Mexico |
| title_sort | use of real time polymerase chain reaction to identify entamoeba histolytica in schoolchildren from northwest mexico |
| topic | Entamoeba histolytica real-time PCR real time PCR school children schoolchildren identification |
| url | https://jidc.org/index.php/journal/article/view/9350 |
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