To elucidate the effect of Ruanjian Qingmai granules on arteriosclerosis obliterans from the perspective of cholesterol efflux
Aim of the studyTo investigate the effect of Ruanjian Qingmai granules (RJQM) on arteriosclerotic obliterans (ASO) and identify its potential bioactive components.Materials and methodsSeparate zebrafish atherosclerosis models and cellular lipid metabolism disorder models were established, and RJQM w...
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Frontiers Media S.A.
2025-08-01
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| Series: | Frontiers in Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2025.1510927/full |
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| author | Chenglin Jia Biying Hong Yujie Jiang Chao Ma Wei Liu Wei Liu Wei Liu Yicheng Xu Jian Chen Yan Xie Yan Xie Yan Xie Guangbo Ge Guangbo Ge Guangbo Ge Ye-min Cao Tianhua Yan Yongbing Cao |
| author_facet | Chenglin Jia Biying Hong Yujie Jiang Chao Ma Wei Liu Wei Liu Wei Liu Yicheng Xu Jian Chen Yan Xie Yan Xie Yan Xie Guangbo Ge Guangbo Ge Guangbo Ge Ye-min Cao Tianhua Yan Yongbing Cao |
| author_sort | Chenglin Jia |
| collection | DOAJ |
| description | Aim of the studyTo investigate the effect of Ruanjian Qingmai granules (RJQM) on arteriosclerotic obliterans (ASO) and identify its potential bioactive components.Materials and methodsSeparate zebrafish atherosclerosis models and cellular lipid metabolism disorder models were established, and RJQM was administered at different concentrations for intervention. The lipid deposition was examined by using Nile Red staining. The expression levels of cholesterol metabolism-related genes were determined by using quantitative real-time PCR (qRT-PCR). The CYP7A1 inhibitor was utilized to elucidate the target of RJQM. Through network pharmacology and serum pharmacochemistry approaches, potential bioactive components were systematically identified and subsequently validated through experimental assays.ResultsRuanjian Qingmai granules significantly decreased lipid deposition and significantly increased the expression of CYP7A1 mRNA in both zebrafish and HepaRG cells. And this effect was attenuated by CYP7A1 inhibitors. Serum pharmacochemistry and network pharmacological analysis indicated that kaempferol and isorhamnetin were potential bioactive components in RJQM for the treatment of ASO. Both components could significantly reduce lipid deposition in zebrafish and HepaRG cells, and this effect was also diminished by CYP7A1 inhibitors. Molecular docking also confirmed that CYP7A1 might be the target of kaempferol and isorhamnetin, and qRT-PCR results also verified that both components could significantly up-regulate the mRNA expression level of CYP7A1.ConclusionRuanjian Qingmai granules exerts a therapeutic effect on ASO by up-regulating the expression level of CYP7A1 mRNA, thereby reprogramming lipid metabolism. Kaempferol and isorhamnetin are likely the main active components of RJQM in lipid metabolic reprogramming. |
| format | Article |
| id | doaj-art-b7307d76dacb4a6691dfb9c2f8938127 |
| institution | DOAJ |
| issn | 2296-858X |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Medicine |
| spelling | doaj-art-b7307d76dacb4a6691dfb9c2f89381272025-08-20T03:00:19ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2025-08-011210.3389/fmed.2025.15109271510927To elucidate the effect of Ruanjian Qingmai granules on arteriosclerosis obliterans from the perspective of cholesterol effluxChenglin Jia0Biying Hong1Yujie Jiang2Chao Ma3Wei Liu4Wei Liu5Wei Liu6Yicheng Xu7Jian Chen8Yan Xie9Yan Xie10Yan Xie11Guangbo Ge12Guangbo Ge13Guangbo Ge14Ye-min Cao15Tianhua Yan16Yongbing Cao17Shanghai TCM-Integrated Hospital, Institute of Vascular Diseases, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaDepartment of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing, ChinaDepartment of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing, ChinaShanghai TCM-Integrated Hospital, Institute of Vascular Diseases, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaShuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaResearch Center for Health and Nutrition, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaInstitute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaShanghai TCM-Integrated Hospital, Institute of Vascular Diseases, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaShanghai TCM-Integrated Hospital, Institute of Vascular Diseases, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaShuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaResearch Center for Health and Nutrition, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaInstitute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaShuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaResearch Center for Health and Nutrition, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaInstitute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaShanghai TCM-Integrated Hospital, Institute of Vascular Diseases, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaDepartment of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing, ChinaShanghai TCM-Integrated Hospital, Institute of Vascular Diseases, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaAim of the studyTo investigate the effect of Ruanjian Qingmai granules (RJQM) on arteriosclerotic obliterans (ASO) and identify its potential bioactive components.Materials and methodsSeparate zebrafish atherosclerosis models and cellular lipid metabolism disorder models were established, and RJQM was administered at different concentrations for intervention. The lipid deposition was examined by using Nile Red staining. The expression levels of cholesterol metabolism-related genes were determined by using quantitative real-time PCR (qRT-PCR). The CYP7A1 inhibitor was utilized to elucidate the target of RJQM. Through network pharmacology and serum pharmacochemistry approaches, potential bioactive components were systematically identified and subsequently validated through experimental assays.ResultsRuanjian Qingmai granules significantly decreased lipid deposition and significantly increased the expression of CYP7A1 mRNA in both zebrafish and HepaRG cells. And this effect was attenuated by CYP7A1 inhibitors. Serum pharmacochemistry and network pharmacological analysis indicated that kaempferol and isorhamnetin were potential bioactive components in RJQM for the treatment of ASO. Both components could significantly reduce lipid deposition in zebrafish and HepaRG cells, and this effect was also diminished by CYP7A1 inhibitors. Molecular docking also confirmed that CYP7A1 might be the target of kaempferol and isorhamnetin, and qRT-PCR results also verified that both components could significantly up-regulate the mRNA expression level of CYP7A1.ConclusionRuanjian Qingmai granules exerts a therapeutic effect on ASO by up-regulating the expression level of CYP7A1 mRNA, thereby reprogramming lipid metabolism. Kaempferol and isorhamnetin are likely the main active components of RJQM in lipid metabolic reprogramming.https://www.frontiersin.org/articles/10.3389/fmed.2025.1510927/fullarteriosclerosis obliteransRuanjian Qingmai granuleslipid reprogrammingCYP7A1potential bioactive components |
| spellingShingle | Chenglin Jia Biying Hong Yujie Jiang Chao Ma Wei Liu Wei Liu Wei Liu Yicheng Xu Jian Chen Yan Xie Yan Xie Yan Xie Guangbo Ge Guangbo Ge Guangbo Ge Ye-min Cao Tianhua Yan Yongbing Cao To elucidate the effect of Ruanjian Qingmai granules on arteriosclerosis obliterans from the perspective of cholesterol efflux Frontiers in Medicine arteriosclerosis obliterans Ruanjian Qingmai granules lipid reprogramming CYP7A1 potential bioactive components |
| title | To elucidate the effect of Ruanjian Qingmai granules on arteriosclerosis obliterans from the perspective of cholesterol efflux |
| title_full | To elucidate the effect of Ruanjian Qingmai granules on arteriosclerosis obliterans from the perspective of cholesterol efflux |
| title_fullStr | To elucidate the effect of Ruanjian Qingmai granules on arteriosclerosis obliterans from the perspective of cholesterol efflux |
| title_full_unstemmed | To elucidate the effect of Ruanjian Qingmai granules on arteriosclerosis obliterans from the perspective of cholesterol efflux |
| title_short | To elucidate the effect of Ruanjian Qingmai granules on arteriosclerosis obliterans from the perspective of cholesterol efflux |
| title_sort | to elucidate the effect of ruanjian qingmai granules on arteriosclerosis obliterans from the perspective of cholesterol efflux |
| topic | arteriosclerosis obliterans Ruanjian Qingmai granules lipid reprogramming CYP7A1 potential bioactive components |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2025.1510927/full |
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