91 The Effect of Pesticide Exposure on Immunological Responses in Children Against SARS-CoV-2
Objectives/Goals: To assess the effect on the immune response to COVID-19 in children exposed to pesticides. The hypothesis is that increased pesticide exposure results in different immunological response to COVID-19. The goal of the proposal is to improve scientific knowledge on factors affecting C...
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| Language: | English |
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Cambridge University Press
2025-04-01
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| Series: | Journal of Clinical and Translational Science |
| Online Access: | https://www.cambridge.org/core/product/identifier/S2059866124007659/type/journal_article |
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| author | Derek Werthmann Elizabeth Norton Felicia Rabito |
| author_facet | Derek Werthmann Elizabeth Norton Felicia Rabito |
| author_sort | Derek Werthmann |
| collection | DOAJ |
| description | Objectives/Goals: To assess the effect on the immune response to COVID-19 in children exposed to pesticides. The hypothesis is that increased pesticide exposure results in different immunological response to COVID-19. The goal of the proposal is to improve scientific knowledge on factors affecting COVID-19 and identify a modifiable factor to reduce these disparities. Methods/Study Population: A cross-sectional analysis of children (aged 5–17 years) with asthma to assess pesticide exposure and immune markers of SARS-CoV-2. SARS-CoV-2 infection or vaccination was determined with blood exposome RNA analyses assessed from blood samples taken at baseline. Immunological response was measured using neutralizing, phagocytizing, and NK-activating antibody responses biomarkers using plasma antibody isotyping, effector functions, T-cell activation-induced marker (AIM), and recall cytokine secretion assays on lysed, whole blood. Pesticide exposure was assessed as concentration of four urinary metabolites in a spot urine sample adjusted for creatinine. Unadjusted regression models were created to assess the effect of 3-phenoxy benzoic acid, a common pyrethroid pesticide, on immune markers. Results/Anticipated Results: Children’s (N = 30) average age was 10 years (interquartile range: 8–11) A majority of children were male (63%) and Non-Hispanic Black (73%). The majority of children had markers of SARS-CoV-2 infection (77%). Of the 4 pesticide metabolites assessed, only 3-PBA was commonly found (77% of samples > LOQ). Higher urinary concentrations of 3-PBA are associated with a significant (p Discussion/Significance of Impact: Significant associations in cytokine and inflammatory marker may indicate a Th2-skewed response, and dysregulated cytokine responses can lead to severe disease. A suggested increase in T-cell activation markers (e.g., CD4, CD8) may indicate potential exhaustion if excessively activated. |
| format | Article |
| id | doaj-art-b72c5ba9fb7945aaad8553a96201de7b |
| institution | Kabale University |
| issn | 2059-8661 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | Journal of Clinical and Translational Science |
| spelling | doaj-art-b72c5ba9fb7945aaad8553a96201de7b2025-08-20T03:40:18ZengCambridge University PressJournal of Clinical and Translational Science2059-86612025-04-019282810.1017/cts.2024.76591 The Effect of Pesticide Exposure on Immunological Responses in Children Against SARS-CoV-2Derek Werthmann0Elizabeth Norton1Felicia Rabito2Tulane University School of Public Health and Tropical MedicineTulane University School of MedicineTulane University School of Public Health and Tropical MedicineObjectives/Goals: To assess the effect on the immune response to COVID-19 in children exposed to pesticides. The hypothesis is that increased pesticide exposure results in different immunological response to COVID-19. The goal of the proposal is to improve scientific knowledge on factors affecting COVID-19 and identify a modifiable factor to reduce these disparities. Methods/Study Population: A cross-sectional analysis of children (aged 5–17 years) with asthma to assess pesticide exposure and immune markers of SARS-CoV-2. SARS-CoV-2 infection or vaccination was determined with blood exposome RNA analyses assessed from blood samples taken at baseline. Immunological response was measured using neutralizing, phagocytizing, and NK-activating antibody responses biomarkers using plasma antibody isotyping, effector functions, T-cell activation-induced marker (AIM), and recall cytokine secretion assays on lysed, whole blood. Pesticide exposure was assessed as concentration of four urinary metabolites in a spot urine sample adjusted for creatinine. Unadjusted regression models were created to assess the effect of 3-phenoxy benzoic acid, a common pyrethroid pesticide, on immune markers. Results/Anticipated Results: Children’s (N = 30) average age was 10 years (interquartile range: 8–11) A majority of children were male (63%) and Non-Hispanic Black (73%). The majority of children had markers of SARS-CoV-2 infection (77%). Of the 4 pesticide metabolites assessed, only 3-PBA was commonly found (77% of samples > LOQ). Higher urinary concentrations of 3-PBA are associated with a significant (p Discussion/Significance of Impact: Significant associations in cytokine and inflammatory marker may indicate a Th2-skewed response, and dysregulated cytokine responses can lead to severe disease. A suggested increase in T-cell activation markers (e.g., CD4, CD8) may indicate potential exhaustion if excessively activated.https://www.cambridge.org/core/product/identifier/S2059866124007659/type/journal_article |
| spellingShingle | Derek Werthmann Elizabeth Norton Felicia Rabito 91 The Effect of Pesticide Exposure on Immunological Responses in Children Against SARS-CoV-2 Journal of Clinical and Translational Science |
| title | 91 The Effect of Pesticide Exposure on Immunological Responses in Children Against SARS-CoV-2 |
| title_full | 91 The Effect of Pesticide Exposure on Immunological Responses in Children Against SARS-CoV-2 |
| title_fullStr | 91 The Effect of Pesticide Exposure on Immunological Responses in Children Against SARS-CoV-2 |
| title_full_unstemmed | 91 The Effect of Pesticide Exposure on Immunological Responses in Children Against SARS-CoV-2 |
| title_short | 91 The Effect of Pesticide Exposure on Immunological Responses in Children Against SARS-CoV-2 |
| title_sort | 91 the effect of pesticide exposure on immunological responses in children against sars cov 2 |
| url | https://www.cambridge.org/core/product/identifier/S2059866124007659/type/journal_article |
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