Reevaluating the role of Pou3f1 in striatal development: Evidence from transgenic mouse models
The striatum, a critical component of the basal ganglia, is essential for motor control, cognitive processing, and emotional regulation. Medium spiny neurons (MSNs) are the primary neuronal population in the striatum, classified into D1 and D2 subtypes. The transcription factor Pou3f1 has been hypot...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-05-01
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| Series: | Brain Research Bulletin |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0361923025001145 |
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| author | Xiaolei Song Fengzhu Zhang Danyu Han Jingzhe Yu Qian Ren Xiaoming Xin Rongliang Guo Weidong Le |
| author_facet | Xiaolei Song Fengzhu Zhang Danyu Han Jingzhe Yu Qian Ren Xiaoming Xin Rongliang Guo Weidong Le |
| author_sort | Xiaolei Song |
| collection | DOAJ |
| description | The striatum, a critical component of the basal ganglia, is essential for motor control, cognitive processing, and emotional regulation. Medium spiny neurons (MSNs) are the primary neuronal population in the striatum, classified into D1 and D2 subtypes. The transcription factor Pou3f1 has been hypothesized to play a crucial role in the development of pyramidal neurons. Recently, a comprehensive analysis of the human embryonic scRNA-seq dataset predicted and emphasized the bridging function of POU3F1 between striatal progenitor cells and immature neurons, though this finding lacked genetic validation. In this study, we found that Pou3f1 expression was significantly reduced after Six3 deletion. However, Pou3f1 deletion does not significantly affect the number or subtype composition of MSNs, nor the proliferation and differentiation of progenitor cells, in our Pou3f1 conditional knockout (cko) mice, challenging the in silico predictions based on human data. These results suggest that Pou3f1 is not required for the specification, generation, or differentiation of MSNs, though its potential involvement in other aspects of striatal development cannot be entirely ruled out. |
| format | Article |
| id | doaj-art-b72a84f7052b4c0f95e903dad31ea05f |
| institution | Kabale University |
| issn | 1873-2747 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brain Research Bulletin |
| spelling | doaj-art-b72a84f7052b4c0f95e903dad31ea05f2025-08-20T03:42:41ZengElsevierBrain Research Bulletin1873-27472025-05-0122411130210.1016/j.brainresbull.2025.111302Reevaluating the role of Pou3f1 in striatal development: Evidence from transgenic mouse modelsXiaolei Song0Fengzhu Zhang1Danyu Han2Jingzhe Yu3Qian Ren4Xiaoming Xin5Rongliang Guo6Weidong Le7Center for Clinical and Translational Medicine, Shanghai University of Medicine and Health Sciences, Shanghai, China; Shanghai University of Medicine & Health Sciences Affiliated Zhoupu Hospital, Shanghai, China; Corresponding authors at: Center for Clinical and Translational Medicine, Shanghai University of Medicine and Health Sciences, Shanghai, China.Shanghai Yangpu District Mental Health Center, Shanghai University of Medicine and Health Sciences, Shanghai, ChinaState Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Institutes of Brain Science, Fudan University, Shanghai, ChinaState Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Institutes of Brain Science, Fudan University, Shanghai, ChinaCenter for Clinical and Translational Medicine, Shanghai University of Medicine and Health Sciences, Shanghai, ChinaCenter for Clinical and Translational Medicine, Shanghai University of Medicine and Health Sciences, Shanghai, China; Shanghai University of Medicine and Health Sciences, Shanghai, ChinaDepartment of Central Laboratory, Affiliated Hospital of Hebei Engineering University, Handan, Hebei, ChinaCenter for Clinical and Translational Medicine, Shanghai University of Medicine and Health Sciences, Shanghai, China; Shanghai University of Medicine & Health Sciences Affiliated Zhoupu Hospital, Shanghai, China; Corresponding authors at: Center for Clinical and Translational Medicine, Shanghai University of Medicine and Health Sciences, Shanghai, China.The striatum, a critical component of the basal ganglia, is essential for motor control, cognitive processing, and emotional regulation. Medium spiny neurons (MSNs) are the primary neuronal population in the striatum, classified into D1 and D2 subtypes. The transcription factor Pou3f1 has been hypothesized to play a crucial role in the development of pyramidal neurons. Recently, a comprehensive analysis of the human embryonic scRNA-seq dataset predicted and emphasized the bridging function of POU3F1 between striatal progenitor cells and immature neurons, though this finding lacked genetic validation. In this study, we found that Pou3f1 expression was significantly reduced after Six3 deletion. However, Pou3f1 deletion does not significantly affect the number or subtype composition of MSNs, nor the proliferation and differentiation of progenitor cells, in our Pou3f1 conditional knockout (cko) mice, challenging the in silico predictions based on human data. These results suggest that Pou3f1 is not required for the specification, generation, or differentiation of MSNs, though its potential involvement in other aspects of striatal development cannot be entirely ruled out.http://www.sciencedirect.com/science/article/pii/S0361923025001145Striatal developmentMSNPou3f1LGE |
| spellingShingle | Xiaolei Song Fengzhu Zhang Danyu Han Jingzhe Yu Qian Ren Xiaoming Xin Rongliang Guo Weidong Le Reevaluating the role of Pou3f1 in striatal development: Evidence from transgenic mouse models Brain Research Bulletin Striatal development MSN Pou3f1 LGE |
| title | Reevaluating the role of Pou3f1 in striatal development: Evidence from transgenic mouse models |
| title_full | Reevaluating the role of Pou3f1 in striatal development: Evidence from transgenic mouse models |
| title_fullStr | Reevaluating the role of Pou3f1 in striatal development: Evidence from transgenic mouse models |
| title_full_unstemmed | Reevaluating the role of Pou3f1 in striatal development: Evidence from transgenic mouse models |
| title_short | Reevaluating the role of Pou3f1 in striatal development: Evidence from transgenic mouse models |
| title_sort | reevaluating the role of pou3f1 in striatal development evidence from transgenic mouse models |
| topic | Striatal development MSN Pou3f1 LGE |
| url | http://www.sciencedirect.com/science/article/pii/S0361923025001145 |
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