Diazotrophic Bacterial Community of Degraded Pastures
Pasture degradation can cause changes in diazotrophic bacterial communities. Thus, this study aimed to evaluate the culturable and total diazotrophic bacterial community, associated with regions of the rhizosphere and roots of Brachiaria decumbens Stapf. pastures in different stages of degradation....
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Applied and Environmental Soil Science |
| Online Access: | http://dx.doi.org/10.1155/2017/2561428 |
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| author | João Tiago Correia Oliveira Everthon Fernandes Figueredo Williane Patrícia da Silva Diniz Lucianne Ferreira Paes de Oliveira Pedro Avelino Maia de Andrade Fernando Dini Andreote Júlia Kuklinsky-Sobral Danúbia Ramos de Lima Fernando José Freire |
| author_facet | João Tiago Correia Oliveira Everthon Fernandes Figueredo Williane Patrícia da Silva Diniz Lucianne Ferreira Paes de Oliveira Pedro Avelino Maia de Andrade Fernando Dini Andreote Júlia Kuklinsky-Sobral Danúbia Ramos de Lima Fernando José Freire |
| author_sort | João Tiago Correia Oliveira |
| collection | DOAJ |
| description | Pasture degradation can cause changes in diazotrophic bacterial communities. Thus, this study aimed to evaluate the culturable and total diazotrophic bacterial community, associated with regions of the rhizosphere and roots of Brachiaria decumbens Stapf. pastures in different stages of degradation. Samples of roots and rhizospheric soil were collected from slightly, partially, and highly degraded pastures. McCrady’s table was used to obtain the Most Probable Number (MPN) of bacteria per gram of sample, in order to determine population density and calculate the Shannon-Weaver diversity index. The diversity of total diazotrophic bacterial community was determined by the technique of Denaturing Gradient Gel Electrophoresis (DGGE) of the nifH gene, while the diversity of the culturable diazotrophic bacteria was determined by the Polymerase Chain Reaction (BOX-PCR) technique. The increase in the degradation stage of the B. decumbens Stapf. pasture did not reduce the population density of the cultivated diazotrophic bacterial community, suggesting that the degradation at any degree of severity was highly harmful to the bacteria. The structure of the total diazotrophic bacterial community associated with B. decumbens Stapf. was altered by the pasture degradation stage, suggesting a high adaptive capacity of the bacteria to altered environments. |
| format | Article |
| id | doaj-art-b7254db605754324b2b050b52986a8ca |
| institution | OA Journals |
| issn | 1687-7667 1687-7675 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applied and Environmental Soil Science |
| spelling | doaj-art-b7254db605754324b2b050b52986a8ca2025-08-20T02:19:19ZengWileyApplied and Environmental Soil Science1687-76671687-76752017-01-01201710.1155/2017/25614282561428Diazotrophic Bacterial Community of Degraded PasturesJoão Tiago Correia Oliveira0Everthon Fernandes Figueredo1Williane Patrícia da Silva Diniz2Lucianne Ferreira Paes de Oliveira3Pedro Avelino Maia de Andrade4Fernando Dini Andreote5Júlia Kuklinsky-Sobral6Danúbia Ramos de Lima7Fernando José Freire8Microbial Genetics and Biotechnology Laboratory, Academic Unit of Garanhuns, Rural Federal University of Pernambuco, 55290-000 Garanhuns, PE, BrazilMicrobial Genetics and Biotechnology Laboratory, Academic Unit of Garanhuns, Rural Federal University of Pernambuco, 55290-000 Garanhuns, PE, BrazilMicrobial Genetics and Biotechnology Laboratory, Academic Unit of Garanhuns, Rural Federal University of Pernambuco, 55290-000 Garanhuns, PE, BrazilMicrobial Genetics and Biotechnology Laboratory, Academic Unit of Garanhuns, Rural Federal University of Pernambuco, 55290-000 Garanhuns, PE, BrazilDepartment of Agricultural Microbiology, Superior School of Agriculture “Luiz de Queiroz”, University of São Paulo, 13418-900 Piracicaba, SP, BrazilDepartment of Agricultural Microbiology, Superior School of Agriculture “Luiz de Queiroz”, University of São Paulo, 13418-900 Piracicaba, SP, BrazilMicrobial Genetics and Biotechnology Laboratory, Academic Unit of Garanhuns, Rural Federal University of Pernambuco, 55290-000 Garanhuns, PE, BrazilDepartment of Agronomy, Rural Federal University of Pernambuco, 52171-900 Recife, PE, BrazilDepartment of Agronomy, Rural Federal University of Pernambuco, 52171-900 Recife, PE, BrazilPasture degradation can cause changes in diazotrophic bacterial communities. Thus, this study aimed to evaluate the culturable and total diazotrophic bacterial community, associated with regions of the rhizosphere and roots of Brachiaria decumbens Stapf. pastures in different stages of degradation. Samples of roots and rhizospheric soil were collected from slightly, partially, and highly degraded pastures. McCrady’s table was used to obtain the Most Probable Number (MPN) of bacteria per gram of sample, in order to determine population density and calculate the Shannon-Weaver diversity index. The diversity of total diazotrophic bacterial community was determined by the technique of Denaturing Gradient Gel Electrophoresis (DGGE) of the nifH gene, while the diversity of the culturable diazotrophic bacteria was determined by the Polymerase Chain Reaction (BOX-PCR) technique. The increase in the degradation stage of the B. decumbens Stapf. pasture did not reduce the population density of the cultivated diazotrophic bacterial community, suggesting that the degradation at any degree of severity was highly harmful to the bacteria. The structure of the total diazotrophic bacterial community associated with B. decumbens Stapf. was altered by the pasture degradation stage, suggesting a high adaptive capacity of the bacteria to altered environments.http://dx.doi.org/10.1155/2017/2561428 |
| spellingShingle | João Tiago Correia Oliveira Everthon Fernandes Figueredo Williane Patrícia da Silva Diniz Lucianne Ferreira Paes de Oliveira Pedro Avelino Maia de Andrade Fernando Dini Andreote Júlia Kuklinsky-Sobral Danúbia Ramos de Lima Fernando José Freire Diazotrophic Bacterial Community of Degraded Pastures Applied and Environmental Soil Science |
| title | Diazotrophic Bacterial Community of Degraded Pastures |
| title_full | Diazotrophic Bacterial Community of Degraded Pastures |
| title_fullStr | Diazotrophic Bacterial Community of Degraded Pastures |
| title_full_unstemmed | Diazotrophic Bacterial Community of Degraded Pastures |
| title_short | Diazotrophic Bacterial Community of Degraded Pastures |
| title_sort | diazotrophic bacterial community of degraded pastures |
| url | http://dx.doi.org/10.1155/2017/2561428 |
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