A label-free fluorescence sensor for bleomycin detection based on a cavity hairpin thioflavin T-light nucleic acid switch
Accurate monitoring of bleomycin (BLM) levels in clinical samples is essential for enhancing therapeutic outcomes while minimizing potential toxicity. In this study, we present a label-free fluorescent sensor for the sensitive detection of BLM, based on a cavity hairpin nucleic acid switch that acti...
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KeAi Communications Co., Ltd.
2025-06-01
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| Series: | Biomedical Analysis |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2950435X25000332 |
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| author | Nian Luo Xueying Li Li Zou |
| author_facet | Nian Luo Xueying Li Li Zou |
| author_sort | Nian Luo |
| collection | DOAJ |
| description | Accurate monitoring of bleomycin (BLM) levels in clinical samples is essential for enhancing therapeutic outcomes while minimizing potential toxicity. In this study, we present a label-free fluorescent sensor for the sensitive detection of BLM, based on a cavity hairpin nucleic acid switch that activates thioflavin T (ThT) fluorescence. The engineered switch features two CGG-AAA cavities that facilitate ThT binding and fluorescence enhancement, along with a BLM-specific cleavage site (5’-GT-3’). Upon exposure to BLM, the BLM·Fe(Ⅱ)-induced DNA cleavage disrupts the hairpin structure, resulting in ThT release and a marked reduction in fluorescence intensity. Under optimized conditions, the sensor exhibits a strong linear correlation between fluorescence intensity and the logarithmic BLM concentration within the range of 10–1000 nM, achieving a detection limit of 1.26 nM. Furthermore, successful application of the sensor in human serum samples demonstrates its potential for clinical diagnostics and biomedical research. |
| format | Article |
| id | doaj-art-b720e137aac243ab814ce63e9f38c7ea |
| institution | DOAJ |
| issn | 2950-435X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Biomedical Analysis |
| spelling | doaj-art-b720e137aac243ab814ce63e9f38c7ea2025-08-20T03:15:22ZengKeAi Communications Co., Ltd.Biomedical Analysis2950-435X2025-06-0122626610.1016/j.bioana.2025.06.003A label-free fluorescence sensor for bleomycin detection based on a cavity hairpin thioflavin T-light nucleic acid switchNian Luo0Xueying Li1Li Zou2School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaSchool of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaSchool of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China; The Second Clinical Medical School of Guangdong Pharmaceutical University (Guangdong Second Provincial General Hospital), Guangzhou 510317, China; Corresponding author at: School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.Accurate monitoring of bleomycin (BLM) levels in clinical samples is essential for enhancing therapeutic outcomes while minimizing potential toxicity. In this study, we present a label-free fluorescent sensor for the sensitive detection of BLM, based on a cavity hairpin nucleic acid switch that activates thioflavin T (ThT) fluorescence. The engineered switch features two CGG-AAA cavities that facilitate ThT binding and fluorescence enhancement, along with a BLM-specific cleavage site (5’-GT-3’). Upon exposure to BLM, the BLM·Fe(Ⅱ)-induced DNA cleavage disrupts the hairpin structure, resulting in ThT release and a marked reduction in fluorescence intensity. Under optimized conditions, the sensor exhibits a strong linear correlation between fluorescence intensity and the logarithmic BLM concentration within the range of 10–1000 nM, achieving a detection limit of 1.26 nM. Furthermore, successful application of the sensor in human serum samples demonstrates its potential for clinical diagnostics and biomedical research.http://www.sciencedirect.com/science/article/pii/S2950435X25000332Label-freeFluorescence sensorBleomycinThioflavin TNucleic acid switch |
| spellingShingle | Nian Luo Xueying Li Li Zou A label-free fluorescence sensor for bleomycin detection based on a cavity hairpin thioflavin T-light nucleic acid switch Biomedical Analysis Label-free Fluorescence sensor Bleomycin Thioflavin T Nucleic acid switch |
| title | A label-free fluorescence sensor for bleomycin detection based on a cavity hairpin thioflavin T-light nucleic acid switch |
| title_full | A label-free fluorescence sensor for bleomycin detection based on a cavity hairpin thioflavin T-light nucleic acid switch |
| title_fullStr | A label-free fluorescence sensor for bleomycin detection based on a cavity hairpin thioflavin T-light nucleic acid switch |
| title_full_unstemmed | A label-free fluorescence sensor for bleomycin detection based on a cavity hairpin thioflavin T-light nucleic acid switch |
| title_short | A label-free fluorescence sensor for bleomycin detection based on a cavity hairpin thioflavin T-light nucleic acid switch |
| title_sort | label free fluorescence sensor for bleomycin detection based on a cavity hairpin thioflavin t light nucleic acid switch |
| topic | Label-free Fluorescence sensor Bleomycin Thioflavin T Nucleic acid switch |
| url | http://www.sciencedirect.com/science/article/pii/S2950435X25000332 |
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